Efficacy of imidacloprid/permethrin and fipronil/(S)–methoprene combinations against Haemaphysalis longicornis ticks evaluated under in vitro and in vivo conditions

Abstract

Haemaphysalis longicornis is one of the most important ticks infesting a wide range of mammals including dogs in Japan. H. longicornis is recorded to be a vector of, for example, Babesia gibsoni. It was the aim of the study presented here to evaluate the efficacy of imidacloprid/permethrin and fipronil/(S)-methoprene against larval, nymphal and adult stages of H. longicornis under in vitro as well as in vivo conditions. In the in vitro part of the study, ticks showed avoidance behaviour to imidacloprid/permethrin-treated filter papers. The onset of acaricidal efficacy in the imidacloprid/permethrin group was recorded earlier than in the fipronil/(S)-methoprene group. In the in vivo experiment three beagles per group were treated with either imidacloprid/permethrin, fipronil/(S)-methoprene or left untreated. Each dog was infested with 30 adult female H. longicornis. Ticks were place on a shaved area of skin of the treated dogs and behaviour of the ticks was recorded as before. After 3 h all ticks were removed and placed in Petri dishes. Ticks were further examined until day 4 post-treatment (p.t.). All ticks recovered from the untreated dogs survived. At 4 h p.t. (1 h post-removal) 40 of the 90 ticks exposed to the imidacloprid/permethrin treatment and 25 of the 90 ticks in the fipronil/(S)-methoprene-treated group were found dead. At day 1 p.t., 61 ticks in the imidacloprid/permethrin- and 81 ticks in the fipronil/(S)-methoprene-treated group were recorded dead. At the final examination day 4 p.t., all 90 ticks were found dead in the imidacloprid/permethrin group, while five ticks remained alive in the fipronil/(S)-methoprene group.

Seroprevalence of Tick-Borne Infections in Military Working Dogs in the Republic of Korea

“…36 Synthetic pyrethroids not only quickly kill ticks but also have tick repellant activity as well. 36,[96][97][98][99] In experimental studies, deltamethrin collars were proven to be effective for greater than five months at controlling infestations with Ixodes ricinus and Rhipicephalus sanguineus ticks. 100 There have been numerous studies looking at the efficacy of a spot-on preparation of imidacloprid plus permethrin with regards to control of ticks.…”

“…96,97,99,[101][102][103][104][105] However, unlike the imidacloprid plus permethrin combination, fipronil did not demonstrate any tick-repellant activity. [96][97][98][99]…”

Efeitos neurocomportamentais da exposição prolongada de ratos ao fibronil

“…Esse praguicida possui amplo espectro de ação e foi desenvolvido para inibir seletivamente receptores do ácido gama amino butírico (GABA) associado a canais de cloreto de insetos (GARSSADI et al, 1994;ZHAO et al, 2004;DRYDEN et al,2005;HAGIMORI et al, 2005;KOCKI et al,2005;RAYMOND-DELPECH et al, 2005;GROSMAN et al, 2006). O GABA é um neurotransmissor inibitório que regula a excitabilidade de todos os neurônios do sistema nervoso central e tem sido associado a eventos fisiológicos e fisiopatológicos que envolvem a função e/ou a disfunção cerebral.…”

Data collection for risk assessments on animal health (Acronym: DACRAH) : Final Report

“…Fipronil EC 424-610-5 (Hagimori et al, 2005) Substance is fatal if inhaled, is toxic if swallowed, is toxic in contact with skin, causes damage to organs through prolonged or repeated exposure, is very toxic to External usetopic/spray A combination of fipronil/(S)-methoprene had an efficacy against H. longicornis of 0.92 4 days post-treatment…”