Efficacy of hot water drenches of <i>Anthurium andraeanum</i> plants against the burrowing nematode <i>Radopholus similis</i> and plant thermotolerance

Tsang, M. M. C.; Kara, Arnold H.; Sipes, B. S.

doi:10.1111/j.1744-7348.2004.tb00388.x

Cited by 14 publications

(8 citation statements)

References 12 publications

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“…Baermann funnels (Viglierchio and Schmitt 1983) were used to separate nematodes from the precipitation and microscope inspection was employed. If there were nematodes in roots, the roots were treated to remove nematodes (Tsang et al 2004). All noncontaminated seedlings were grown in sterilized soil medium for 15 days for later use.…”

Section: Plant Materialsmentioning

confidence: 99%

Differential expression of Rs-eng-1b in two populations of Radopholus similis (Tylenchida: Pratylecnchidae) and its relationship to pathogenicity

Zhang

Xie

et al. 2012

Eur J Plant Pathol

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We constructed a SSH (suppression subtractive hybridization) library based on two populations (Rs-C and Rs-P) of Radopholus similis from different host plants and exhibiting differences in pathogenicity on Musa paradisiaca and Anthurium andraeanum plants. In order to screen the clones with significant expression differences from the SSH library, a total of 2,400 clones was randomly selected and reverse northern blotting was performed on them. Out of the 2,400 clones, 89 clones showed significant expression differences. Out of sequencing these 89 clones, distinct sequences from 87 clones were obtained. Aligning the 87 distinct sequences against the non-redundant nucleotide database (nr) in NCBI, we found that five sequences were highly conserved with Rs-eng-1b. Two of five sequences with lengths of 467 base pairs (bp) (GW395922) and 742 bp (GW395923) were further employed to perform 5′ RACE-PCR and 3′ RACE-PCR, respectively. Subsequently, the complete length of Rs-eng-1b (EU414839) was obtained (1,427 bp). Our qPCR result showed that expression of Rs-eng-1b in the population Rs-C with high pathogenicity on host plants was approximately 2.7 times as much as the expression of Rs-eng-1b in the population Rs-P with low pathogenicity on host plants. Furthermore, the gene Rs-eng-1b from the Rs-C population also showed expression differences amongst four different development stages. The order of Rs-eng-1b relative expression abundance from high to low was females, juveniles, males, and eggs. We further used RNAi to test whether Rs-eng-1b of Rs-C population was responsible for pathogenicity which was the first RNAi work about Rs-eng-1b. The RNAi results showed that Rs-eng-1b expression had a positive correlation to pathogenicity of the population. The longer the RNAi treatment, the less pathogenic the nematode population was. Non-endogenous gfp dsRNA had no significant influence on the expression of Rs-eng-1b and pathogenicity of R. similis Rs-C population. In conclusion, all our evidence indicated Rs-eng-1b might be a crucial pathogenicity-related gene in R. similis.

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Section: Plant Materialsmentioning

confidence: 99%

Differential expression of Rs-eng-1b in two populations of Radopholus similis (Tylenchida: Pratylecnchidae) and its relationship to pathogenicity

Zhang

Xie

et al. 2012

Eur J Plant Pathol

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“…Complete mortality of J2 of M. incognita was achieved at 44 and 45 o C when treated for one hour. Tsang et al (2003;2004) showed that hot water drenches disinfect roots and media of potted bamboo or palms by eliminating all the burrowing nematode, Radopholus similis. They found that a continuous hot water (50°C) drenching of either bare roots or media in pots for 15 min (bamboo palm) or 13 min (fishtail palms) successfully eliminated all burrowing nematodes.…”

Section: Discussionmentioning

confidence: 99%

Development of a hot water disinfestation treatment for taro exported from the Pacific Islands

Jamieson¹,

Chhagan²,

Redpath³

et al. 2016

NZPP

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Taro imported to New Zealand from the Pacific Islands are currently fumigated with methyl bromide (MB) for control of mites nematodes and other quarantine pests There are growing restrictions on the use of MB and alternatives to this fumigant are being sought such as hot water treatments Mites (Rhizoglyphus minutus) and nematodes (Meloidogyne sp or Helicotylenchus sp) were exposed to hot water both on and off taro Hot water exposures ranged from 475C to 525C for 15 s to 5 min the duration depending on the temperature Preliminary off taro experiments indicated that 100 mortality of R minutus mite adults was achieved in 34 min at 475C and between 30 s and 1 min at 50C All adult mites were dead at the shortest treatment duration (15 s) at 525C The results indicated that 100 mortality of juvenile Meloidogyne sp was achieved in 12 min at 475C and between 30 s and 1 min at 50C All juvenile nematodes were dead at 45 s at 525C Preliminary on taro experiments at 525C for durations of 35 min and 5 min indicated that a longer duration would be required to achieve 100 mortality of Rhizoglyphus mites No live Meloidogyne or Helicotylenchus nematodes were present in either the untreated or hot watertreated taro

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“…Baermann funnels were used to separate the nematodes from the precipitate, and a microscopic inspection was performed [ 28 ]. If nematodes were present in the roots, the roots were soaked in hot water at 62°C to remove the nematodes [ 15 ]. All of the uncontaminated seedlings were grown in sterilized soil medium for 15 days for later use.…”

Section: Methodsmentioning

confidence: 99%

Molecular Identification and Functional Characterization of the Fatty Acid- and Retinoid-Binding Protein Gene Rs-far-1 in the Burrowing Nematode Radopholus similis (Tylenchida: Pratylenchidae)

et al. 2015

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Fatty acid- and retinoid-binding protein (FAR) is a nematode-specific protein expressed in the nematode hypodermis. It is involved in nematode development, reproduction, and infection and can disrupt the plant defense reaction. In this study, we obtained the full-length sequence of the far gene from Radopholus similis (Rs-far-1), which is 828 bp long and includes a 558 bp ORF encoding 186 amino acids. A protein homology analysis revealed that Rs-FAR-1 is 75% similar to Mj-FAR-1 from Meloidogyne javanica. A neighbor-joining phylogenetic tree was inferred and showed that Rs-FAR-1 is most similar to Pv-FAR-1 from Pratylenchus vulnus. A fluorescence-based ligand-binding analysis confirmed that Rs-FAR-1 can combine with fatty acids and retinol. qPCR was used to assess Rs-far-1 expression levels at different developmental stages in different R. similis populations, and its expression was 2.5 times greater in the highly pathogenic Rs-C population than in the less pathogenic Rs-P population. The highest expression was found in females, followed by eggs, juveniles and males. When R. similis was treated with Rs-far-1 dsRNA for 36 h, the reproduction and pathogenicity decreased significantly. In situ hybridization revealed Rs-far-1 transcripts in the R. similis hypodermis. Additionally, R. similis treated with Rs-far-1 dsRNA or water were inoculated into Arabidopsis thaliana. Allene oxide synthase (AOS) expression in A. thaliana was upregulated during early infection in both treatments and then returned to the expression levels of the control plant. Compared with the control plant, AOS expression significantly decreased in A. thaliana inoculated with water-treated R. similis but significantly increased in A. thaliana inoculated with Rs-far-1 dsRNA-treated R. similis. This finding indicates that Rs-far-1 regulates AOS expression in A. thaliana. Rs-FAR-1 plays a critical role in R. similis development, reproduction, and infection and can disturb the plant defense reaction. Therefore, Rs-far-1 is an important target gene to control R. similis.

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Efficacy of hot water drenches of Anthurium andraeanum plants against the burrowing nematode Radopholus similis and plant thermotolerance

Cited by 14 publications

References 12 publications

Differential expression of Rs-eng-1b in two populations of Radopholus similis (Tylenchida: Pratylecnchidae) and its relationship to pathogenicity

Differential expression of Rs-eng-1b in two populations of Radopholus similis (Tylenchida: Pratylecnchidae) and its relationship to pathogenicity

Development of a hot water disinfestation treatment for taro exported from the Pacific Islands

Molecular Identification and Functional Characterization of the Fatty Acid- and Retinoid-Binding Protein Gene Rs-far-1 in the Burrowing Nematode Radopholus similis (Tylenchida: Pratylenchidae)

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