Efficacy of hESC-MSCs in knitted silk-collagen scaffold for tendon tissue engineering and their roles

Chen, Jia Lin; Yin, Zi; Shen, Wei; Chen, Xiao; Heng, Boon Chin; Zou, Xiao Hui

doi:10.1016/j.biomaterials.2010.08.011

Cited by 207 publications

(184 citation statements)

References 52 publications

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“…The latest studies are searching for other factors that could be of use and one of these is the successfully tested chondroitin sulfate C (ref. 30 ). The differentiation procedure we followed in our study 3 proved useful in our previous experiments and it was further tested in other studies 4 .…”

Section: Discussionmentioning

confidence: 99%

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

Plánka¹,

Srnec²,

Raušer³

et al. 2012

BIOMED PAP

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Introduction. This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. Material and methods. The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. Results. Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases. Conclusions. The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.

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Section: Discussionmentioning

confidence: 99%

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

Plánka¹,

Srnec²,

Raušer³

et al. 2012

BIOMED PAP

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“…Because of the limited availability of SCs, there are also some alternative cell types, including BMSCs, hair follicle stem cells, olfactory ensheathing cells, adipose-and skin-derived stem cells [100]. In biofabrication of neural tissues, the optimal cell types involved are neural stem cells, mesenchymal stem cells (MSCs) and induced pluripotent stem cells [104], due to their capacity to differentiate into any neural cell type [105]. As demonstrated in Table 8, over the last years, several materials have been developed for bioprinting of neural constructs.…”

Section: Bioprinting Of Neural Tissuesmentioning

confidence: 99%

3D bioprinting for cell culture and tissue fabrication

Jian

Wang

et al. 2018

Bio-des. Manuf.

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Three-dimensional (3D) bioprinting is a computer-assisted technology which precisely controls spatial position of biomaterials, growth factors and living cells, offering unprecedented possibility to bridge the gap between structurally mimic tissue constructs and functional tissues or organoids. We briefly focus on diverse bioinks used in the recent progresses of biofabrication and 3D bioprinting of various tissue architectures including blood vessel, bone, cartilage, skin, heart, liver and nerve systems. This paper provides readers a guideline with the conjunction between bioinks and the targeted tissue or organ types in structuration and final functionalization of these tissue analogues. The challenges and perspectives in 3D bioprinting field are also illustrated.

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“…Pneumatic actuators, [19][20][21] linear motors, 22,27 and step motorball screws (SMBSs) 2,42,76 are the most common actuators used in tendon/ligament bioreactors. Pneumatic actuators have several advantages, including the ease of maintenance, cleanliness, low cost, and high power-to-weight ratio.…”

Section: Actuator and Culture Chamber Designmentioning

confidence: 99%

“…In the United States, injuries to tendons and ligaments represent about half of the 33 million musculoskeletal injuries. 1 Each year more than 33,000 tendon reconstructions occur in the United States, costing $30 billion USD 2,3 and in Australia, $250 million is spent annually just on rotator cuff repair. 4 However, despite the high prevalence of tendon injury and associated tendinopathy worldwide, treatment options remain poorly defined.…”

Section: Introductionmentioning

confidence: 99%

“…29 A recent study demonstrated that embryonic stem cells (ESCs) can be induced to exhibit a tenocyte-like morphology and express tendon-related gene markers if exposed to an appropriate biomechanical environment. 2 Unfortunately, the clinical application of ESCs is restricted due to their limited availability (depending on the policy of different countries) and the complexity of cell manipulation required. Dermal fibroblasts have also been shown to form tendon tissue, [30][31][32] although further research has suggested that the healing process using skin-derived fibroblasts is suppressed with a lack of tenocyte markers and histopathologic correlations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioreactor Design for Tendon/Ligament Engineering

Wang

Gardiner

Lin

et al. 2013

Tissue Engineering Part B: Reviews

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Tendon and ligament injury is a worldwide health problem, but the treatment options remain limited. Tendon and ligament engineering might provide an alternative tissue source for the surgical replacement of injured tendon. A bioreactor provides a controllable environment enabling the systematic study of specific biological, biochemical, and biomechanical requirements to design and manufacture engineered tendon/ligament tissue. Furthermore, the tendon/ligament bioreactor system can provide a suitable culture environment, which mimics the dynamics of the in vivo environment for tendon/ligament maturation. For clinical settings, bioreactors also have the advantages of less-contamination risk, high reproducibility of cell propagation by minimizing manual operation, and a consistent end product. In this review, we identify the key components, design preferences, and criteria that are required for the development of an ideal bioreactor for engineering tendons and ligaments.

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Efficacy of hESC-MSCs in knitted silk-collagen scaffold for tendon tissue engineering and their roles

Cited by 207 publications

References 52 publications

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

3D bioprinting for cell culture and tissue fabrication

Bioreactor Design for Tendon/Ligament Engineering

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