Efficacy of forming biofilms by Pseudomonas migulae AN-1 toward in situ bioremediation of aniline-contaminated aquifer by groundwater circulation wells

Zhao, Yongsheng; Qu, Dan; Zhou, Rui; Yang, Shuai; Ren, Hejun

doi:10.1007/s11356-016-6737-7

Cited by 24 publications

(6 citation statements)

References 31 publications

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“…Additionally, P. stutzeri has been identified in and used in a number of consortia-based applications, such as bioremediation ( Nguyen et al, 2008 ; Brodie et al, 2011 ; Gałazka et al, 2012 ), wastewater denitrification ( Clarens et al, 1998 ; Liu et al, 2006 ), aquaculture water quality management ( Erna et al, 2013 ; Deng et al, 2014 ), as a plant growth promoter for the biocontrol of phytopathogens ( Cottyn et al, 2009 ; Shen et al, 2013 ), and manufacturing/municipal waste management ( Miyahara et al, 2012 ; Moscoso et al, 2012b ). The use of P. stutzeri biofilms for various bioremediation efforts has potential benefits in terms of activity and yields for copper removal ( Badar et al, 2013 ), drinking water denitrification ( Lazarova et al, 1992 ) and naphthalene ( Zhao et al, 2016 ), and phenol ( Viggiani et al, 2006 ) degradation. Given this widespread utilization in both microbial consortia systems and biofilms, P. stutzeri was selected to demonstrate the deconstruction of its interactions with other pseudomonas in biofilm-based co-cultures into the genetic and chemical aspects influencing the co-colony fitness.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

et al. 2021

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Pseudomonas species are ubiquitous in nature and include numerous medically, agriculturally and technologically beneficial strains of which the interspecific interactions are of great interest for biotechnologies. Specifically, co-cultures containing Pseudomonas stutzeri have been used for bioremediation, biocontrol, aquaculture management and wastewater denitrification. Furthermore, the use of P. stutzeri biofilms, in combination with consortia-based approaches, may offer advantages for these processes. Understanding the interspecific interaction within biofilm co-cultures or consortia provides a means for improvement of current technologies. However, the investigation of biofilm-based consortia has been limited. We present an adaptable and scalable method for the analysis of macroscopic interactions (colony morphology, inhibition, and invasion) between colony-forming bacterial strains using an automated printing method followed by analysis of the genes and metabolites involved in the interactions. Using Biofilm Interaction Mapping and Analysis (BIMA), these interactions were investigated between P. stutzeri strain RCH2, a denitrifier isolated from chromium (VI) contaminated soil, and 13 other species of pseudomonas isolated from non-contaminated soil. One interaction partner, Pseudomonas fluorescens N1B4 was selected for mutant fitness profiling of a DNA-barcoded mutant library; with this approach four genes of importance were identified and the effects on interactions were evaluated with deletion mutants and mass spectrometry based metabolomics.

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Section: Introductionmentioning

confidence: 99%

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

et al. 2021

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“…Additionally, P. stutzeri has been discovered and used in a number of consortia based applications, such as bioremediation (19)(20)(21), wastewater denitrification (22,23), aquaculture water quality management (24,25), as a plant growth promoter for the biocontrol of phytopathogens (26,27) and manufacturing/municipal waste management (28,29). The use of P. stutzeri biofilms for various bioremediation efforts has potential benefits in terms of activity and yields for copper removal (30), drinking water denitrification (31) and naphthalene (32) and phenol (33) degradation. Given this widespread utilization in both microbial consortia systems and biofilms, P. stutzeri was selected to demonstrate the deconstruction of its interactions with other pseudomonas in biofilm-based co-cultures into the genetic and chemical aspects influencing the co-colony fitness.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Interaction Mapping and Analysis (BIMA): A tool for deconstructing interspecific interactions in co-culture biofilms

Kosina

Rademacher

Wetmore

et al. 2021

Preprint

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Pseudomonas species are ubiquitous in nature and include numerous medically, agriculturally and technologically beneficial strains of which the interspecific interactions are of great interest for biotechnologies. Specifically, co-cultures containing Pseudomonas stutzeri have been used for bioremediation, biocontrol, aquaculture management and wastewater denitrification. Furthermore, the use of P. stutzeri biofilms, in combination with consortia based approaches, may offer advantages for these processes. Understanding the interspecific interaction within biofilm co-cultures or consortia provides a means for improvement of current technologies. However, the investigation of biofilm based consortia has been limited. We present an adaptable and scalable method for the analysis of macroscopic interactions (colony morphology, inhibition and invasion) between colony forming bacterial strains using an automated printing method followed by analysis of the genes and metabolites involved in the interactions. Using Biofilm Interaction Mapping and Analysis (BIMA), these interactions were investigated between P. stutzeri strain RCH2, a denitrifier isolated from chromium (VI) contaminated soil, and thirteen other species of pseudomonas isolated from non-contaminated soil. The metabolites and genes associated with both active co-culture growth and inhibitory growth were investigated using mass spectrometry based metabolomics and mutant fitness profiling of a DNA-barcoded mutant library. One interaction partner, Pseudomonas fluorescens N1B4 was selected for mutant fitness profiling; with this approach four genes of importance were identified and the effects on interactions were evaluated with deletion mutants and metabolomics.

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“…Note that in recent years, new technologies taking VCW as in‐well bioreactors for aquifer remediation have been actively explored. For example, Yuan et al (2020) and Zhao et al (2016) employed VCW to experimentally conduct microbially enhanced degradation and groundwater electrolysis investigations, respectively.…”

Section: Introductionmentioning

confidence: 99%

Optimization Strategies for in Situ Groundwater Remediation by a Vertical Circulation Well Based on Particle‐Tracking and Node‐Dependent Finite Difference Methods

Wen

et al. 2020

Water Resources Research

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Both theory and application of multispecies reactive transport for in situ groundwater bioremediation involving a vertical circulation well (VCW) are not fully understood despite its importance and common usage in aquifer remediation practices. This study proposes novel approaches including two methods for design and remediation prediction of a VCW system, which involves multispecies, multiphase, and microbially enhanced reactive transport process. One is particle-tracking method, which depicts the trajectories of particles released from an injection chamber; the other is node-dependent finite difference (NDFD) method, which describes the advection-dispersion process based on the inflow and outflow directions at each node. The numerical results demonstrate that the particle-tracking method works well by yielding a useful index, that is, the recovery ratio, which helps optimize the in situ preliminary remediation screening. When biochemical parameters, dispersivities, and in situ contaminated conditions are known after the preliminary screening, the NDFD method performs better than the conventional Laplace transform finite difference method in terms of describing multispecies reactive transport with multiple phases in a VCW system. The proposed particle-tracking method and NDFD methods are employed to elucidate different effects of factors such as injection mode, hydraulic conductivity anisotropy ratio, distance between injection and extraction screened intervals, and injection/extraction rate on recovery and removal ratios. Our findings suggest that both methods are effective tools for optimization and prediction of VCW remediation in an anisotropic aquifer.

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Efficacy of forming biofilms by Pseudomonas migulae AN-1 toward in situ bioremediation of aniline-contaminated aquifer by groundwater circulation wells

Cited by 24 publications

References 31 publications

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

Biofilm Interaction Mapping and Analysis (BIMA): A tool for deconstructing interspecific interactions in co-culture biofilms

Optimization Strategies for in Situ Groundwater Remediation by a Vertical Circulation Well Based on Particle‐Tracking and Node‐Dependent Finite Difference Methods

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