Efficacy of Fine-Needle Aspiration Cytology in the Diagnosis of Tuberculous Cervical Lymphadenitis

Rammeh, Soumaya; Toumi, Amira Arfaoui; Houcine, Yoldez; Lahiani, Rym; Sassi, Asma; Mardassi, Helmi; Salah, Mamia Ben; Ferjaoui, M.

doi:10.1159/000487503

Cited by 10 publications

(3 citation statements)

References 16 publications

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“…Tuberculosis is diagnosed based on the presence or absence of necrotic granulomas and epithelioid cell granulomas, and dark specks and darkened elliptical objects are sometimes seen. 11,18 Moreover, a definite diagnosis of TB is made based on the finding of MTB by an examination of ZN staining or culture. However, in patients with negative AFB, diagnosis is difficult.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical Study of Rabbit-Polyclonal to Mycobacterium Tuberculosis, AB905: Improving Diagnostic of Tuberculous Lymphadenitis

Lubis¹,

Purwoningsih²,

Roslina³

et al. 2021

MMJKK

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Tuberculous lymphadenitis (TBLN) is the most common form of extrapulmonary tuberculosis. However, the optimal diagnosis using Fine-Needle Aspiration Cytology (FNAC) or excisional biopsy is uncertain. This research aims to improve the diagnostic of TBLN with FNAC and immunocytochemistry (ICC) compared to the response to antituberculosis therapy. The cross-sectional study involved 43 patients with the criteria for TBLN diagnosis based on the appropriate clinical history of tuberculosis and indicative cytological results. Immunocytochemical examination employed rabbit-polyclonal to Mycobacterium tuberculosis (MTB) antibody (AB905). The MTB expression was found in 35 out of 43 cases (81%) that appropriate cytological features of the tuberculosis process. Meanwhile, eight out of 43 cases (19%) did not express MTB. Diagnostic tests for lesions with a positive cytologic appearance of TBLN and ICC were compared to the response to anti-tuberculosis therapy, revealing the sensitivity, specificity, positive predictive value, and negative predictive value of 95.2%, 75%, 95.2%, and 17%, respectively. Besides, Fisher's exact tests utilized to identify the relationship between two variables; p 0.05 was considered significant. This research found immunocytochemical study was a sensitive and specific tool for improving the diagnostic of TBLN.

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Section: Discussionmentioning

confidence: 99%

Immunocytochemical Study of Rabbit-Polyclonal to Mycobacterium Tuberculosis, AB905: Improving Diagnostic of Tuberculous Lymphadenitis

Lubis¹,

Purwoningsih²,

Roslina³

et al. 2021

MMJKK

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“…In our patient, diagnosis of TL was based either on the identification of Mycobacterium tuberculosis in the pus of the draining sinuses of the lymph node or on the histopathologic examination of the lymph node showing a typical pathology of tuberculosis. For the accurate diagnosis of cervical TL, the contribution of fine-needle aspiration cytology (FNAC), while avoiding any complication associated with the open biopsy, is reported in the relevant literature [15, 16]. The use of fine-needle aspiration cytology in diagnosis of extrapulmonary tuberculous lymphadenitis was also reported by previous studies [12, 17–19], with granulomatous inflammation on fine-needle aspiration cytology to be strongly indicative for tuberculosis infection [18].…”

Section: Discussionmentioning

confidence: 99%

Left Lateral Cervical Mass with Draining Sinuses

Michaelides

Bablekos

Michailidis

et al. 2019

Case Reports in Medicine

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The aim of the present study is to describe an uncommon case of tuberculous lymphadenitis (TL) in a symptomless 89-year-old male smoker patient, who presented at the emergency department of our hospital with left lateral cervical swelling with draining sinuses. No other clinical symptoms or physical findings were observed at admission. An elevated erythrocyte sedimentation rate (ESR) and a small calcified nodule in chest CT were the only abnormal findings. Pus samples from sinuses were examined and confirmed tuberculosis which was in agreement with surgical pathology of lymph nodes. A four- (4-) drug antituberculous regimen was administered. After an initial remission of his symptoms, the patient presented an exacerbation of the cervical swelling with draining sinuses necessitating addition of oral steroids. TL can be symptomless presenting a paradoxical reaction during treatment. The uniqueness of our case lies in the patient’s advanced age, which is uncommon with cervical lymphadenopathy as a form of extrapulmonary tuberculosis, as well as in the administration of oral steroids to resolve the neck’s clinical deterioration. The patient had a complete recovery and was free of disease after completion of his six-month antituberculous chemotherapy.

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“…Tuberculous lymphadenitis accounts for nearly 35% of extra-pulmonary TB. The diagnosis of tuberculous lymphadenitis can be morphologically by fine needle aspiration cytology (FNAC) of the lymph node [2].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Microwave-Heated Ziehl-Neelsen Stain and Conventional Ziehl-Neelsen Method in the Detection of Acid-Fast Bacilli in Lymph Node Biopsies

Ahmed¹,

Mohammed²,

Taha³

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND: Tuberculosis is a chronic inflammatory disease with lymphadenopathy being the most common extra-pulmonary manifestation. The conventional Ziehl–Neelsen method plays an essential role in the diagnosis of tuberculosis; however, it has a low sensitivity in detecting acid-fast bacilli. AIM: The present study emphasises the role of the microwave-heated method (modified Ziehl–Neelsen) over conventional Ziehl-Neelsen stain and to set at the best condition for irradiation. MATERIAL AND METHODS: The study included 90 patients with clinically suspected tuberculous lymphadenopathy who were referred to the Department of Pathology at Omdurman Military Hospital, Sudan. Demographic data such as age, sex, and site of swelling were documented for each patient. Specimens were stained with conventional Ziehl-Neelsen, fluoresce and the modified methods. RESULTS: Patient’s age ranged from 20 to 70 year. Of the total 90 cases with clinically suspected tuberculous lymphadenopathy, 18 cases were positive for AFB in conventional Ziehl-Neelsen method giving a sensitivity of 13.3%, while in microwave-heated method 82 cases of TB were detected positive for AFB yielded sensitivity and specificity of 97.6% and 85.7%, respectively, and positive and negative predictive values of 98.8% and 75.0% respectively compared to fluorescence methods. CONCLUSION: In the present study, the microwave-heated Ziehl-Neelsen method, was found to have sensitivity and specificity of 97.6% and 85.7%, respectively which matches the fluorescence technique. It has specificity in detecting lymph node tuberculosis that makes it superior over all other modified methods. However, the availability and cost-effectiveness might limit the use of fluorescence in routine practice. Furthermore, the study set the best staining temperature is provided at power 1 level (60 w) for 1.5 minutes.

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Efficacy of Fine-Needle Aspiration Cytology in the Diagnosis of Tuberculous Cervical Lymphadenitis

Cited by 10 publications

References 16 publications

Immunocytochemical Study of Rabbit-Polyclonal to Mycobacterium Tuberculosis, AB905: Improving Diagnostic of Tuberculous Lymphadenitis

Immunocytochemical Study of Rabbit-Polyclonal to Mycobacterium Tuberculosis, AB905: Improving Diagnostic of Tuberculous Lymphadenitis

Left Lateral Cervical Mass with Draining Sinuses

Comparison of the Microwave-Heated Ziehl-Neelsen Stain and Conventional Ziehl-Neelsen Method in the Detection of Acid-Fast Bacilli in Lymph Node Biopsies

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