Efficacy of early treatment on 52 patients with preneoplastic hepatitis B virus-associated hepatocellular carcinoma by compound Phyllanthus Urinaria L.

Tong, Guangdong; Zhang, Xi; Zhou, Daoguo; Wei, Chunshan; He, Jinsong; Xiao, Chunling; Liu, Xin-liang; Zheng, Yingjun; Chen, Si-nuan; Tang, Haihong

doi:10.1007/s11655-013-1320-7

Cited by 15 publications

(16 citation statements)

References 33 publications

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“…In our previous bioinformatic analysis, we found that VWCE was low expressed in breast cancer based on online analysis tools. These results are not consistent with the previous studies in other tumors [13,14,32,33]. We speculate that it may be related to the role and mechanism of the same gene in different tissues and cells, that is, malignant tumors are highly heterogeneous.…”

Section: Discussioncontrasting

confidence: 98%

VWCE Serves as a Potential Prognostic Biomarker and is Associated With Immune Infiltrates in Breast Cancer

Huo

Xie

2020

Preprint

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Background: Von Willebrand Factor C and EGF Domains (VWCE) is an important gene that regulates cell adhesion, migration, and interaction. However, the correlation between VWCE expression and immune inﬁltrating in breast cancer remain unclear. In the study, we investigated the correlation between VWCE expression and immune infiltration levels in breast cancer.Methods: VWCE expression was analyzed by the tumor immune estimation resource (TIMER) and DriverDB database. Furthermore, genes co-expressed with VWCE and gene ontology (GO) enrichment analysis were investigated by the STRING and Enrichr web servers. Also, we performed the single nucleotide variation (SNV), copy number variation (CNV), and pathway activity analysis by GSCALite. Subsequently, the relationship between VWCE expression and tumor immunity was analyzed by TIMER and TISIDB database, and further verified the results using immunohistochemistry. Results: The results showed that the expression of VWCE mRNA in breast cancer tissue was significantly lower than that in normal tissue. We found that the expression level of VWCE was associated with subtypes, estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2) status of patients with breast cancer, while no significant expression difference of VWCE mRNA was found in age and nodal status. Further analyses indicated that VWCE was correlated with the activation or inhibition of multiple oncogenic pathways. Additionally, VWCE expression showed negatively correlated with the expression of STAT1 (Th1 marker, r = -0.12, p = 6e-05), but positively correlated with the expression of MS4A4A (r = 0.28, p = 0). These results suggested that the expression of VWCE was significantly correlates the immune infiltration levels of Th1 and M2 macrophage in breast cancer. Conclusions: The high expression of VWCE indicates a better prognosis and associated with immune infiltration in breast cancer. These findings demonstrate that VWCE is a potential prognostic biomarker correlated with tumor immune cell infiltration, and maybe a promising therapeutic target in breast cancer.

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Section: Discussioncontrasting

confidence: 98%

VWCE Serves as a Potential Prognostic Biomarker and is Associated With Immune Infiltrates in Breast Cancer

Huo

Xie

2020

Preprint

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“…Once the treatment is stopped, the recurrence rate is 10.4–13.4% respectively (Cheng et al, 2009 ). Tong et al ( 2014 ) reports that compound in capsule of P. urinaria L. suppresses development of hepatocellular carcinoma (HCC) through an improved immune system, reversion of liver fibrosis, blockage of the induced hepatocarcinoma cell cycle and inhibition of angiogenesis. The HBV-DNA levels decrease ≥2 log in 22.2% (10/45) of patients in the treatment group compared with the control group at 5.0% (2/40).…”

Section: Clinical Trials Of P Urinaria Preparatiomentioning

confidence: 99%

“…The HBV-DNA levels decrease ≥2 log in 22.2% (10/45) of patients in the treatment group compared with the control group at 5.0% (2/40). The number of antibodies that test positive in the treated group is lower (1.08 ± 1.01) after the treatment period of 24 months compared with the control group (2.11 ± 1.12) (Tong et al, 2014 ). The anti-URG11 (33/52) and anti-URG19 (31/52) in both treated and control groups are over 60% at base line.…”

Section: Clinical Trials Of P Urinaria Preparatiomentioning

confidence: 99%

A Review of the Phytochemistry and Pharmacology of Phyllanthus urinaria L.

Geethangili

Ding

2018

Front. Pharmacol.

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The genus Phyllanthus (L.) is one of the most important groups of plants belonging to the Phyllantaceae family. Phyllanthus urinaria (L.) is an annual perennial herbal species found in tropical Asia, America, China, and the Indian Ocean islands. P. urinaria is used in folk medicine as a cure to treat jaundice, diabetes, malaria, and liver diseases. This review provides traditional knowledge, phytochemistry, and biological activities of P. urinaria. The literature reviewed for this article was obtained from the Web of Science, SciFinder, PubMed, ScienceDirect, and Google Scholar journal papers published prior to December 2017. Phytochemical investigations reveal that the plant is a rich source of lignans, tannins, flavonoids, phenolics, terpenoids, and other secondary metabolites. Pharmacological activities include anticancer, hepatoprotective, antidiabetic, antimicrobial, and cardioprotective effects. Thus, this present review summarizes the phytochemical constituents and their biological activities including biological studies on various crude extracts and fractions both in vitro and in vivo, and on clinical trial information about P. urinaria. This review compiles 93 naturally occurring compounds from P. urinaria along with their structures and pharmacological activities. The review is expected to stimulate further research on P. urinaria, and its pharmacological potential to yield novel therapeutic agents.

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“…It is a formula used for HCC, especially HBV-related HCC in China. In our previous studies, we have elucidated that CP could reduce the expression of HBxAg in transplant tumors, and we also verified that CP could inhibit HBV-DNA replication and reduce the expression of URG4, URG7, URG11, and URG9, preventing and delaying the development of HBV-related HCC in patients treated with CP for two years [16–18]. Other scholars proved that CP could inhibit expressions of HBsAg and HBeAg, and it also could enhance the immune response in vivo and in vitro [19].…”

Section: Discussionmentioning

confidence: 80%

Compound Phyllanthus urinaria L Inhibits HBV-Related HCC through HBx-SHH Pathway Axis Inactivation

Jiang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Compound Phyllanthus urinaria L (CP) is a traditional formula widely used in clinical practice for hepatocellular carcinoma (HCC), especially HBV-related HCC. HBx, HBV X gene encoded X protein, has positive correlation with the abnormal SHH pathway in HBV-related HCC. So, we predicted that CP has the capability of anti-HBV-related HCC maybe via inactivating the HBx-Hedgehog pathway axis. HepG2-HBx cells, HBx overexpression, were treated with CP (70μg/ml and 35 μg/ml, respectively) for 48 hours and the mice which received the HepG2-HBx cells were treated with CP (625mg/kg and 300 mg/kg, respectively) for 17 days to evaluate the effect of CP on HBV-related HCC. HBx could accelerate HepG2 cells proliferation, clone formation, and migration in vitro and also could strengthen tumor growth in mice. However, CP could significantly decrease HepG2-HBx cells proliferation, clone formation, and migration in vitro and also could inhibit tumors growth in mice in a dose-dependent manner. Mechanism studies suggested that HBx upregulated the mRNA and proteins expression of Sonic hedgehog (SHH), transmembrane receptor patched (PTCH-1), smoothened (SMO), oncogene homolog transcription factors-1 (GLI-1), and oncogene homolog transcription factors-2 (GLI-2), which are compositions of the SHH pathway. CP could inhibit the mRNA and proteins expression of SHH, PTCH-1, GLI-1, and HBx. It may be one of the underlying mechanisms of CP to delay the HBV-related HCC development through the HBx-SHH pathway axis inactivation.

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Efficacy of early treatment on 52 patients with preneoplastic hepatitis B virus-associated hepatocellular carcinoma by compound Phyllanthus Urinaria L.

Abstract: Anti-URG11, anti-URG19 and anti-DRG2 could be used as early markers in the prediction of the therapeutic efficacy of CPUL in treating preneoplastic HCC. CPUL is useful in preventing or delaying the development of HBV-associated cirrhosis to HCC.

Cited by 15 publications

References 33 publications

VWCE Serves as a Potential Prognostic Biomarker and is Associated With Immune Infiltrates in Breast Cancer

VWCE Serves as a Potential Prognostic Biomarker and is Associated With Immune Infiltrates in Breast Cancer

A Review of the Phytochemistry and Pharmacology of Phyllanthus urinaria L.

Compound Phyllanthus urinaria L Inhibits HBV-Related HCC through HBx-SHH Pathway Axis Inactivation

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Efficacy of early treatment on 52 patients with preneoplastic hepatitis B virus-associated hepatocellular carcinoma by compound Phyllanthus Urinaria L.

Abstract: Anti-URG11, anti-URG19 and anti-DRG2 could be used as early markers in the prediction of the therapeutic efficacy of CPUL in treating preneoplastic HCC. CPUL is useful in preventing or delaying the development of HBV-associated cirrhosis to HCC.

Cited by 15 publications

References 33 publications

VWCE&nbsp;Serves as a Potential Prognostic Biomarker and is Associated With Immune Infiltrates in Breast Cancer

VWCE&nbsp;Serves as a Potential Prognostic Biomarker and is Associated With Immune Infiltrates in Breast Cancer

A Review of the Phytochemistry and Pharmacology of Phyllanthus urinaria L.

Compound Phyllanthus urinaria L Inhibits HBV-Related HCC through HBx-SHH Pathway Axis Inactivation

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VWCE Serves as a Potential Prognostic Biomarker and is Associated With Immune Infiltrates in Breast Cancer

VWCE Serves as a Potential Prognostic Biomarker and is Associated With Immune Infiltrates in Breast Cancer