Efficacy of Double Filtration Plasmapheresis in Removing Xenoantibodies and Prolonging Xenograft Survival in an Ex Vivo Swine Perfusion Model

Lee, Y.-T.; C, Chu; Sue, Sung-How; Chen, I.-C.; Wei, Jih Hua

doi:10.1016/j.transproceed.2012.02.012

Cited by 2 publications

(3 citation statements)

References 10 publications

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“…59 In addition, an increase in WBC counts was also observed during perfusions of pig hearts, although the nature of this rise was not further characterized. 60 At last, perfusion of pig lungs with different perfusion solutions showed the release of pig T-and B lymphocytes as well as monocytes, macrophages, and dendritic cells. 61 In summary, the nature of porcine cells released during xenoperfusion of different organs remains poorly characterized.…”

Section: Discussionmentioning

confidence: 99%

“…An early publication indicated that porcine leukocytes, mainly lymphocytes, were released from pig kidneys during buffered‐saline perfusion . In addition, an increase in WBC counts was also observed during perfusions of pig hearts, although the nature of this rise was not further characterized . At last, perfusion of pig lungs with different perfusion solutions showed the release of pig T‐ and B lymphocytes as well as monocytes, macrophages, and dendritic cells .…”

Section: Discussionmentioning

confidence: 99%

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Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA‐E/human CD46 double‐transgenic pig limbs with human blood

Yung

Bongoni

Pradier

et al. 2017

Xenotransplantation

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Ex vivo perfusion of pig forelimbs using whole human blood represents a powerful tool to study humoral and early cell-mediated rejection mechanisms of vascularized pig-to-human xenotransplantation, although there are several limitations of the model. Here, we show that (i) transgenic expression of HLA-E/hCD46 in pig limbs provides partial protection from human NK cell-mediated xeno responses and (ii) the emergence of a pig cell population during xenoperfusions with implications for the immunogenicity of xenografts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA‐E/human CD46 double‐transgenic pig limbs with human blood

Yung

Bongoni

Pradier

et al. 2017

Xenotransplantation

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“…Two non-Gal antigens, Neu5Gc (N-glycolylneuraminic acid) and SDa blood group, are known to be responsible for AHXR ( 19 – 21 ). In the present immunotherapy protocols, HAR and AHXR can be avoided using plasmapheresis and use of pigs genetically modified for the deletion of α-Gal and the two non-Gal antigens (triple knockout or TKO) ( 5 , 22 , 23 ). Acute cellular xenograft rejection (ACXR), which involves NK cells, macrophages, neutrophils, T-cells and B-Cells, also remains major hurdle in long-term xenograft survival ( 5 ).…”

Section: Advanced Immunosuppression Protocols For Xenotransplant Trialsmentioning

confidence: 99%

Xenotransplantation: A New Era

et al. 2022

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Organ allotransplantation has now reached an impassable ceiling inherent to the limited supply of human donor organs. In the United States, there are currently over 100,000 individuals on the national transplant waiting list awaiting a kidney, heart, and/or liver transplant. This is in contrast with only a fraction of them receiving a living or deceased donor allograft. Given the morbidity, mortality, costs, or absence of supportive treatments, xenotransplant has the potential to address the critical shortage in organ grafts. Last decade research efforts focused on creation of donor organs from pigs with various genes edited out using CRISPR technologies and utilizing non-human primates for trial. Three groups in the United States have recently moved forward with trials in human subjects and obtained initial successful results with pig-to-human heart and kidney xenotransplantation. This review serves as a brief discussion of the recent progress in xenotransplantation research, particularly as it concerns utilization of porcine heart, renal, and liver xenografts in clinical practice.

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Efficacy of Double Filtration Plasmapheresis in Removing Xenoantibodies and Prolonging Xenograft Survival in an Ex Vivo Swine Perfusion Model

Cited by 2 publications

References 10 publications

Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA‐E/human CD46 double‐transgenic pig limbs with human blood

Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA‐E/human CD46 double‐transgenic pig limbs with human blood

Xenotransplantation: A New Era

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