Efficacy of DMARDs and methylprednisolone treatment on the gene expression levels of HSPA5, MMD, and non-coding RNAs MALAT1, H19, miR-199a-5p, and miR-1-3p, in patients with rheumatoid arthritis

Mahmoudi, Zohreh; Karamali, Negin; Roghani, Seyed Askar; Assar, Shirin; Pournazari, Mehran; Soufivand, Parviz; Salari, Farhad; Rezaiemanesh, Alireza

doi:10.1016/j.intimp.2022.108878

Cited by 12 publications

(10 citation statements)

References 45 publications

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“…Previous studies have shown that miR-1-3p is closely related to the pathogenesis of autoimmune diseases as well as several other diseases. As an example, the expression levels of MALAT1 and miR-1-3p were significantly higher in newly diagnosed patients with rheumatoid arthritis than NC [ 33 ]. Some studies have also shown that overexpression of miR-1-3p might facilitate Th17 differentiation by inhibiting the expression of ETS1 in naïve CD4 + T cells [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and bioinformatics analysis of lncRNAs in serum of patients with ankylosing spondylitis

Kou,

Bie,

Liu

et al. 2024

BMC Musculoskelet Disord

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Objectives The aim of this study was to explore the long non-coding RNA (lncRNA) expression profiles in serum of patients with ankylosing spondylitis (AS). The role of these lncRNAs in this complex autoimmune situation needs to be evaluated. Methods We used high-throughput whole-transcriptome sequencing to generate sequencing data from three patients with AS and three normal controls (NC). Then, we performed bioinformatics analyses to identify the functional and biological processes associated with differentially expressed lncRNAs (DElncRNAs). We confirmed the validity of our RNA-seq data by assessing the expression of eight lncRNAs via quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 20 AS and 20 NC samples. We measured the correlation between the expression levels of lncRNAs and patient clinical index values using the Spearman correlation test. Results We identified 72 significantly upregulated and 73 significantly downregulated lncRNAs in AS patients compared to NC. qRT-PCR was performed to validate the expression of selected DElncRNAs; the results demonstrated that the expression levels of MALAT1:24, NBR2:9, lnc-DLK1-35:13, lnc-LARP1-1:1, lnc-AIPL1-1:7, and lnc-SLC12A7-1:16 were consistent with the sequencing analysis results. Enrichment analysis showed that DElncRNAs mainly participated in the immune and inflammatory responses pathways, such as regulation of protein ubiquitination, major histocompatibility complex class I-mediated antigen processing and presentation, MAPkinase activation, and interleukin-17 signaling pathways. In addition, a competing endogenous RNA network was constructed to determine the interaction among the lncRNAs, microRNAs, and mRNAs based on the confirmed lncRNAs (MALAT1:24 and NBR2:9). We further found the expression of MALAT1:24 and NBR2:9 to be positively correlated with disease severity. Conclusion Taken together, our study presents a comprehensive overview of lncRNAs in the serum of AS patients, thereby contributing novel perspectives on the underlying pathogenic mechanisms of this condition. In addition, our study predicted MALAT1 has the potential to be deeply involved in the pathogenesis of AS.

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Section: Discussionmentioning

confidence: 99%

Identification and bioinformatics analysis of lncRNAs in serum of patients with ankylosing spondylitis

Kou,

Bie,

Liu

et al. 2024

BMC Musculoskelet Disord

View full text Add to dashboard Cite

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“…Recently, the emerging role of long non‐coding RNAs (lncRNAs) in the initiation and progression of chronic inflammatory states such as RA has been reported 5,6 . Multiple studies have shown dysregulation of lncRNAs such as H19 , Neat1 and identified them as potential bio‐markers in collagen induced arthritis (CIA)/OA/RA patients or in animal models 7–10 . H19 is known to be upregulated [~2.75‐fold] in joint tissue and plasma samples of RA patients and is a key player in the activation of inflammatory pathways 11 .…”

Section: Figurementioning

confidence: 99%

“…5,6 Multiple studies have shown dysregulation of lncRNAs such as H19, Neat1 and identified them as potential bio-markers in collagen induced arthritis (CIA)/OA/RA patients or in animal models. [7][8][9][10] H19 is known to be upregulated [~2.75-fold] in joint tissue and plasma samples of RA patients and is a key player in the activation of inflammatory pathways. 11 Similarly, Neat1 is upregulated [~1.…”

mentioning

confidence: 99%

Potential role of long non‐coding RNA H19 and Neat1 in haemophilic arthropathy

Sarangi

Senthilkumar

Kumar

et al. 2023

J Cellular Molecular Medi

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“…Quercetin may exhibit the protective activity against MALAT1-induced pathological changes in RA FLS ( 92 ). It has been reported that MALAT1 can be considered as a potential biomarker for RA diagnosis, due to being statistically correlating with the expression of HSPA5 (GRP78) and MMD (PAQR1) in patients with RA ( 93 ). However, the single nucleotide polymorphism of MALAT1 gene is reported to be not correlated with the susceptibility of RA ( 94 ).…”

Section: The Roles Of Malat1 In Rheumatoid Arthritismentioning

confidence: 99%

The regulatory activities of MALAT1 in the development of bone and cartilage diseases

2022

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Long non-coding RNAs (lncRNAs) have been comprehensively implicated in various cellular functions by mediating transcriptional or post-transcriptional activities. MALAT1 is involved in the differentiation, proliferation, and apoptosis of multiple cell lines, including BMSCs, osteoblasts, osteoclasts, and chondrocytes. Interestingly, MALAT1 may interact with RNAs or proteins, regulating cellular processes. Recently, MALAT1 has been reported to be associated with the development of bone and cartilage diseases by orchestrating the signaling network. The involvement of MALAT1 in the pathological development of bone and cartilage diseases makes it available to be a potential biomarker for clinical diagnosis or prognosis. Although the potential mechanisms of MALAT1 in mediating the cellular processes of bone and cartilage diseases are still needed for further elucidation, MALAT1 shows great promise for drug development.

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Efficacy of DMARDs and methylprednisolone treatment on the gene expression levels of HSPA5, MMD, and non-coding RNAs MALAT1, H19, miR-199a-5p, and miR-1-3p, in patients with rheumatoid arthritis

Cited by 12 publications

References 45 publications

Identification and bioinformatics analysis of lncRNAs in serum of patients with ankylosing spondylitis

Identification and bioinformatics analysis of lncRNAs in serum of patients with ankylosing spondylitis

Potential role of long non‐coding RNA H19 and Neat1 in haemophilic arthropathy

The regulatory activities of MALAT1 in the development of bone and cartilage diseases

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