Efficacy of Directigen® RSV testing in patient management following admission from a paediatric emergency department

Lipson, Steven M.; Popiolek, Dorota; Hu, Q.Z; Falk, Leon; Bornfreund, Mark; Krilov, Leonard R.

doi:10.1053/jhin.1998.0532

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…Overall, the percentages observed were higher than those obtained (sensitivity 62.5% and specificity 91.8%) in a previous study, carried out with a lower number of samples in the period 1995-1996 [14]. They were also higher than those reported by some other research groups, ranging from 57% [15] to 76% [16], although some authors have reported sensitivity percentages close to 98% [17][18][19][20].…”

Section: Discussioncontrasting

confidence: 73%

Prospective evaluation of a dot-blot enzyme immunoassay (Directigen RSV) for the antigenic detection of respiratory syncytial virus from nasopharyngeal aspirates of paediatric patients

Reina

Gárdenas

Gopegui

et al. 2004

Clinical Microbiology and Infection

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This study investigated the efficacy of a commercial enzyme immunoassay (Directigen RSV, ColorPAC) in comparison with the shell vial culture method (using Hep-2 cells) for the detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirates from children with bronchiolitis. During the period 1995-2002, 4950 samples were examined. RSV was detected in 1660 (33.5%) samples, with a sensitivity of 80.9%, a specificity of 97.5%, a positive predictive value of 93.8%, a negative predictive value of 91.6%, and a testing efficiency value of 92.2% compared with shell vial culture. In 83 (5%) samples, the ColorPAC was positive and the shell vial assay was negative. Of these, 71 (85.6%) were false-negative by cell culture. The true false-positive results obtained by ColorPAC represented only 0.7% of all RSV-positive samples. In general, no statistically significant differences were detected between the different months and epidemic periods studied. Compared with ColorPAC, the shell vial culture method displayed a sensitivity of 95.8% and a specificity of 100%. Overall, the ColorPAC assay was an acceptable, simple and rapid method for the antigenic detection of RSV in paediatric respiratory samples.

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Section: Discussioncontrasting

confidence: 73%

Prospective evaluation of a dot-blot enzyme immunoassay (Directigen RSV) for the antigenic detection of respiratory syncytial virus from nasopharyngeal aspirates of paediatric patients

Reina

Gárdenas

Gopegui

et al. 2004

Clinical Microbiology and Infection

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“…There is a continued effort, accordingly, to reduce specimen turnaround times in order to retain a competitive edge. To achieve this goal, many laboratory administrators direct their facilities to utilize rapid and simple-toperform assays, rather than more-tedious but more-sensitive assays (2,8,9). The impetus to rapid respiratory viral diagnostics is reflected by the widespread use of the numerous enzyme immunoassay kits with detection times shorter than 15 min currently flooding the marketplace (1, 9).…”

Section: Rapid Laboratory Diagnostics During the Winter Respiratory Vmentioning

confidence: 99%

“…Respiratory virus testing utilizing real-time nucleic acid amplification technologies is slowly being introduced to the clinical virology community (12). The technical study by Liolios et al, however, although capable of detecting seven respiratory virus types, fails to address the overwhelming practical issue of real-time (and simple-to-perform) laboratory viral diagnostics (8,9). The described multiplex RT-PCR indeed serves as a valid epidemiological tool, but the assay's 24-to 36-h turnaround time to the detection of respiratory viruses severely limits its routine application in the clinical setting.…”

Section: Rapid Laboratory Diagnostics During the Winter Respiratory Vmentioning

confidence: 99%

Rapid Laboratory Diagnostics during the Winter Respiratory Virus Season

Lipson

2002

J Clin Microbiol

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“…The antigen detection methods offer the potential for rapid diagnosis (within hours) and may be performed reliably in the absence of a sophisticated diagnostic virology laboratory [11]. Monitoring of test performance, however, is critical for maintaining appropriate sensitivity and specificity [12]. Specific tests for RSV may be indicated in a particular patient for making decisions about therapy, cohorting of patients, and educating parents or physicians about the nature of RSV disease.…”

Section: Laboratory Diagnosismentioning

confidence: 99%

Respiratory syncytial virus: Update on infection, treatment, and prevention

Krilov

2001

Curr Infect Dis Rep

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Respiratory syncytial virus (RSV) infection, which primarily manifests as bronchiolitis or pneumonia, is the leading cause of lower respiratory tract infection in infants and young children. It is associated with more than 100,000 pediatric hospitalizations each year in the United States. Infants who were premature; have chronic lung disease, congenital heart disease, or immunodeficiency disorders; or have underlying metabolic or neuromuscular disorders are at increased risk for especially severe RSV disease. Treatment of children hospitalized with RSV disease is primarily supportive, with administration of supplemental oxygen and fluid replacement therapy. Bronchodilators may benefit at least a subset of such patients. Antiviral therapy with aerosolized ribavirin is available for high-risk, severely ill patients. Handwashing, cleaning of environmental surfaces, and cohorting in hospital settings may decrease RSV transmission. In children born premature and younger than 1 year of age, and in patients with bronchopulmonary dysplasia younger than 2 years of age, passive protection against severe RSV disease may be achieved through monthly injections of anti-RSV antibody (palivizumab) during winter months. No vaccine is available to provide active immunity against RSV, but live attenuated and subunit cloned surface protein vaccines are in development.

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Efficacy of Directigen® RSV testing in patient management following admission from a paediatric emergency department

Cited by 8 publications

References 11 publications

Prospective evaluation of a dot-blot enzyme immunoassay (Directigen RSV) for the antigenic detection of respiratory syncytial virus from nasopharyngeal aspirates of paediatric patients

Prospective evaluation of a dot-blot enzyme immunoassay (Directigen RSV) for the antigenic detection of respiratory syncytial virus from nasopharyngeal aspirates of paediatric patients

Rapid Laboratory Diagnostics during the Winter Respiratory Virus Season

Respiratory syncytial virus: Update on infection, treatment, and prevention

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