Efficacy of different entomopathogenic fungal isolates against four key stored-grain beetle species

Wakil, Waqas; Kavallieratos, Nickolas G.; Ghazanfar, Muhammad Usman; Usman, Muhammad; Habib, Amer; El-Shafie, Hamadttu A. F.

doi:10.1016/j.jspr.2021.101845

Cited by 25 publications

(30 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason might be attributed to the fact that strains were isolated from their original host pest and showed a stronger ability to infect the original host compared with other strains isolated from different insects [76]. This proves that isolation of EPF from the target host might have an advantage of discovering highly virulent strains which can only germinate and expand when it infests its specific host, called host specificity [76][77][78]. The reason for host specificity is biological long-term coevolution and mutual adaptation, which may have limited the choices of parasites for the host [77].…”

Section: Discussionmentioning

confidence: 99%

Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata

Zemek

Konopická

Jozová

et al. 2021

Insects

View full text Add to dashboard Cite

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a serious, widely distributed pest of potato and other crops. This pest is able to defoliate the host plant and cause severe yield loss. Moreover, the pest quickly becomes resistant to many chemical pesticides. Therefore, the development of novel biopesticides targeting this pest is urgently needed. The purpose of this study was to obtain new strains of the entomopathogenic fungus Beauveria bassiana and assess their efficacy against L. decemlineata adults under laboratory conditions. Twelve strains were isolated from cadavers of Colorado potato beetles collected in potato fields in the Czech Republic. Test beetles were treated by suspensions of conidia at the concentration of 1 × 107 spores per milliliter and their survival was recorded daily for three weeks. The results of the bioassays revealed that all new native strains were pathogenic to L. decemlineata adults and caused mortality up to 100% at the end of the trial period with an LT50 of about 7 days. These strains were more virulent than a reference strain GHA and some of them can be recommended for the development of a new mycoinsecticide against L. decemlineata. Our findings also highlight the importance of searching for perspective strains of entomopathogenic fungi among naturally infected hosts.

show abstract

Section: Discussionmentioning

confidence: 99%

Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata

Zemek

Konopická

Jozová

et al. 2021

Insects

View full text Add to dashboard Cite

show abstract

“…Management of the aforementioned stored-product insect pests is feasible through the application of insecticides as grain protectants (Kavallieratos et al 2020a(Kavallieratos et al , b, 2021aWakil et al 2021aWakil et al , 2022. Among different types of insecticides used as grain protectants, pyrethroids, organophosphates, and carbamates have been extensively used in storedproduct protection (Attia et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Their mode of action against insects is based on six steps beginning with the percutaneous attachment of the spore, the germination on the host cuticle, the deep penetration inside the host, the weakening of the host immune defense system, the proliferation of the fungi inside the host, and lastly the new conidia emerge from the dead insect (Zimmermann 2007). Previous studies have documented the elevated activity of entomopathogenic fungi against numerous stored-product insect pests (Athanassiou et al 2008a;Shafighi et al 2014;Batta and Kavallieratos 2018;Mohammed et al 2019;Wakil et al 2021aWakil et al , b, c, d, 2022. More specifically, different entomopathogenic fungi isolates of Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae), Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavipitaceae), Lecanicillium attenuatum (Petch) Zare and Gams (Hypocreales: Cordycipitaceae), Isaria fumosorosea (Wize) (Hypocreales: Clavicipitaceae), and Chaetomium globosum (Kunze) (Sordariales: Chaetomiaceae) have been tested as grain protectants against adults of R. dominica, S. granarius, T. granarium, and T. castaneum, the saw-toothed grain beetle, Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae), the rusty grain beetle, Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae), the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae), and the psocid Liposcelis paeta (Pearman) (Psocoptera: Liposcelididae) (Athanassiou et al 2007(Athanassiou et al , 2008aShafighi et al 2014;Saeed et al 2020;Wakil et al 2021a, b, c, d).…”

Section: Introductionmentioning

confidence: 99%

Combinations of Beauveria bassiana and spinetoram for the management of four important stored-product pests: laboratory and field trials

Wakil

Kavallieratos

Nika

et al. 2022

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

The current study examines the efficacy of the semi-synthetic insecticide spinetoram and entomopathogenic fungi Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae) as wheat protectants against the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae), the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), the granary weevil, Sitophilus granarius (L.) (Coleoptera: Curculionidae), and the khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae), under laboratory and field trials. One dose of B. bassiana, i.e., 1 × 107 conidia/kg wheat, two doses of spinetoram, i.e., spine1: 0.05 ppm (mg/kg wheat), spine2: 0.1 ppm, and their combinations (Bb + spine1, Bb + spine2) were evaluated at 20, 25, and 30 °C. All treatments provided significantly higher mortality at 30 °C compared with the other two temperatures. Maximum mortality levels were observed in the treatments where B. bassiana was combined with the higher dose of spinetoram (0.1 ppm). All treatments reduced progeny production in comparison with the control groups. Maximum progeny reduction was observed at 30 °C, on wheat treated with the Bb + spine2 combination. The combination Bb + spine2 also provided elevated mortality rates in both laboratory and field persistence trials, but at 180 days caused moderate mortality to all tested insect species. Concerning progeny, at laboratory persistence trials, the combination Bb + spine2 exhibited the lowest offspring emergence to all tested species compared to the other treatments and control. Overall, our study showed that R. dominica was the most susceptible species followed by S. granarius, T. castaneum, and T. granarium. Our findings revealed that the combination of B. bassiana and spinetoram may be a useful tool for efficient and advanced integrated pest management strategies for long storage periods under multiple temperatures.

show abstract

“…In the natural habitat, EMPF are a diverse group of insect-invading fungal pathogens that can effectively infect and regulate numerous insect pest populations [9][10][11]. In this regard, and to benefit from the evolutionary fine-tuned relationship between EMPF and their host insects, it is necessary to obtain and screen potential EMPF isolates and characterize their important properties such as insect host range, environmental persistence, plant growth impact and other important traits.…”

Section: Introductionmentioning

confidence: 99%

Screening and Identification of Indigenous Entomopathogenic Fungal Isolates from Agricultural Farmland Soils in Nile Delta, Egypt

Alfiky

2022

JoF

View full text Add to dashboard Cite

The compound negative impact of insect pests attacking agricultural ecosystems includes (i) direct yield losses from damaged crops, (ii) the economic cost of the attempt to prevent these losses and (iii) the negative short- and long-term hazard effects of chemical pesticides on human and environmental health. Entomopathogenic fungi (EMPF) are a group of microorganisms that represent the natural enemies of a number of crop pests, presenting an opportunity to harness their evolutionary fine-tuned relationship with their insect hosts as biocontrol agents in integrated pest management programs. The aim of this study was to establish an indigenous EMPF collection via the Galleria mellonella (greater wax moth) entrapment method from the soils of Nile Delta, Egypt. Obtained insect associated fungal isolates were bio-assayed for pathogenicity against the serious pest Spodoptera litura and Tenebrio molitor, and the seven outperforming isolates were selected for molecular identification and thermotolerance assay. Based on ITS sequence analysis and phylogeny, selected isolates were identified as Beauveria bassiana (four isolates), Metarhizium anisopliae (two isolates) and one isolate of Cordyceps javanica. The obtained results demonstrated (i) the efficacy of using insect baiting coupled with molecular identification and pathogenicity screening to isolate EMPF to control insect pests, and (ii) the availability of indigenous virulent EMPF in Nile Delta’s soil, which can be exploited for the development of sustainable crop protection strategies.

show abstract

Efficacy of different entomopathogenic fungal isolates against four key stored-grain beetle species

Cited by 25 publications

References 48 publications

Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata

Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata

Combinations of Beauveria bassiana and spinetoram for the management of four important stored-product pests: laboratory and field trials

Screening and Identification of Indigenous Entomopathogenic Fungal Isolates from Agricultural Farmland Soils in Nile Delta, Egypt

Contact Info

Product

Resources

About