Efficacy of Different Encapsulation Techniques on the Viability and Stability of Diverse Phage under Simulated Gastric Conditions

Dlamini, Sicelo B.; Gigante, Adriano M.; Hooton, Steven P. T.; Atterbury, Robert J.

doi:10.3390/microorganisms11102389

Cited by 2 publications

(1 citation statement)

References 72 publications

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“…Their study demonstrated effective encapsulation (>95%) and maintenance of viability (>87%) of five genetically diverse bacteriophages representing three genera after exposure to simulated gastric conditions (pH 2, 3.2 mg/ml pepsin, 37°C, for 1 hour). All bacteriophages were easily released from microcapsules at pH 7.5 and exposure to simulated duodenal conditions (pH 7, 10 mg/ml pancreatin, 37°C) ( 119 ). Eudragit S100 has been widely used as an encapsulation material for targeted bacteriophage release in the colon ( 5 ).…”

Section: Optimizing Formulation and Encapsulation Strategies For Phag...mentioning

confidence: 99%

Advances and optimization strategies in bacteriophage therapy for treating inflammatory bowel disease

Li,

Duan

et al. 2024

Front. Immunol.

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In the advancement of Inflammatory Bowel Disease (IBD) treatment, existing therapeutic methods exhibit limitations; they do not offer a complete cure for IBD and can trigger adverse side effects. Consequently, the exploration of novel therapies and multifaceted treatment strategies provides patients with a broader range of options. Within the framework of IBD, gut microbiota plays a pivotal role in disease onset through diverse mechanisms. Bacteriophages, as natural microbial regulators, demonstrate remarkable specificity by accurately identifying and eliminating specific pathogens, thus holding therapeutic promise. Although clinical trials have affirmed the safety of phage therapy, its efficacy is prone to external influences during storage and transport, which may affect its infectivity and regulatory roles within the microbiota. Improving the stability and precise dosage control of bacteriophages—ensuring robustness in storage and transport, consistent dosing, and targeted delivery to infection sites—is crucial. This review thoroughly explores the latest developments in IBD treatment and its inherent challenges, focusing on the interaction between the microbiota and bacteriophages. It highlights bacteriophages’ potential as microbiome modulators in IBD treatment, offering detailed insights into research on bacteriophage encapsulation and targeted delivery mechanisms. Particular attention is paid to the functionality of various carrier systems, especially regarding their protective properties and ability for colon-specific delivery. This review aims to provide a theoretical foundation for using bacteriophages as microbiome modulators in IBD treatment, paving the way for enhanced regulation of the intestinal microbiota.

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Section: Optimizing Formulation and Encapsulation Strategies For Phag...mentioning

confidence: 99%

Advances and optimization strategies in bacteriophage therapy for treating inflammatory bowel disease

Li,

Duan

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

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Bacterial defenses and their trade-off with growth are not ubiquitous but depend on ecological contexts

Liu,

Niu

2024

Preprint

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Bacteriophages, also known as bacterial viruses, exert significant influence on microbial ecosystems due to their widespread presence. In response to the threat of phage predation, bacteria have developed an extensive array of antiviral defense mechanisms. This study investigates the intricate relationship between bacterial defense mechanisms and growth rates across various ecological contexts. First, we found that bacteria lacking defense systems show prolonged doubling times. By integrating phylogenetic eigenvectors into the ecological feature matrix, we employed a phylogenetic random forest model to determine the most influential ecological features on the presence and abundance of bacterial defenses. Further phylogenetic regressions unveiled nuanced dependencies of bacterial defenses on specific environmental factors. Contrary to conventional assumptions, our findings challenge the idea of a universal distribution of bacterial defense systems, instead highlighting their reliance on subtle ecological characteristics. Particularly noteworthy are observations that symbiotic and endosymbiotic bacteria often exhibit reduced defense systems and negative correlations between minimal doubling time and defense system abundance. Conversely, as per conventional assumptions, free-living and motile bacteria displayed significant positive correlations between minimal doubling time and defense system abundance. Overall, our results suggest that the trade-off between growth and defense strategies is not universally applicable but rather contingent on context. Moreover, our study illuminates the critical role of ecological variables such as habitat specificity, motility, and nutrient availability in shaping bacterial growth rates and defense mechanisms. These findings underscore the complexity of microbial interactions and stress the importance of considering the ecological context in understanding bacterial defense strategies.

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Efficacy of Different Encapsulation Techniques on the Viability and Stability of Diverse Phage under Simulated Gastric Conditions

Cited by 2 publications

References 72 publications

Advances and optimization strategies in bacteriophage therapy for treating inflammatory bowel disease

Advances and optimization strategies in bacteriophage therapy for treating inflammatory bowel disease

Bacterial defenses and their trade-off with growth are not ubiquitous but depend on ecological contexts

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