Efficacy of desulfated heparin mitigating inflammation in rat burn wound model

Abstract

During skin repair, leukocyte infiltration is the principal inflammatory response which is instrumental in triggering growth factor and cytokine signals that orchestrate together to recruit cells necessary for healing. In severe wounds like burn, when acute inflammation becomes chronic, intervention is required to control inflammation so as to hasten the process of healing. Heparin, a known anticoagulant also possesses anti-inflammatory activity by its ability to interfere with the adhesion of leukocytes to the endothelium. Desulfated heparins (DSH) have subdued anticoagulant activity while possessing increased anti-inflammatory activity. Among which 2,3 DSH is found to have marked potency as an anti-inflammatory agent and has been utilized for this study. In this investigation, a controlled delivery system was designed by incorporating 2,3 DSH in microspheres and embedding in collagen matrix which could serve as a wound dressing in burns. In vivo evaluation of healing process was ascertained in rat burn wound model by qualitative and quantitative estimation of proinflammatory cytokines in serum and granulation tissue and collagen turnover was also assessed as healing progressed. The results of this study suggests that 2,3 DSH could be delivered in a controlled manner to regulate inflammatory events to hasten healing of burn wounds.

Acceleration of skin regeneration in full-thickness burns by incorporation of bFGF-loaded alginate microspheres into a CMCS-PVA hydrogel

“…After being anaesthetized with pentobarbital (30 mg/kg), the dorsal area of the rats was completely denuded with 8% w/v Na 2 S aqueous solution, and four full‐thickness circular wounds were burned with a hot circular copper billet (ф = 15 mm; 90°C, 20 s). Severe burn injury promotes a significant inflammatory response and increases capillary permeability, with intravascular volume deficits especially during the first 24 h. To mimic the clinical treatment of burns, the burns were excised to the level of the panniculus carnosus 24 h later (Kita et al ., ; Lakshmi et al ., ; Lan et al ., ). A total of four full‐thickness burns (diameter 20 mm, 300 mm apart) were induced on the dorsum of each rat.…”

Extracellular Histones Play an Inflammatory Role in Acid Aspiration-induced Acute Respiratory Distress Syndrome

“…Heparin is a glycosaminoglycan well known for its anticoagulant properties. 41 ARDS remains a serious clinical concern around the world. 18,39,40 However, the mechanisms responsible for the antiinflammatory effects of heparin are not well understood.…”

Partially-desulfated heparin improves survival inPseudomonaspneumonia by enhancing bacterial clearance and ameliorating lung injury

“…ODSH has been shown to inhibit the secretion of pro-inflammatory cytokines including tumor necrosis factor (TNF)-a, interleukin (IL)-6, and IL-1b upon stimulation or injury, but has minimal effects on coagulation (Rao et al, 2010;Lakshmi et al, 2011). In addition, inflammatory lung injury mediated by either human leukocyte elastase or HMGB1 can be inhibited by treatment with ODSH (Fryer et al, 1997;Rao et al, 2010).…”