Efficacy of Cosupplementation Therapy With Vitamins B9, B12, and D on Endothelial Dysfunction in Streptozotocin-Induced Diabetic Rats

Abstract

Objective: This study evaluated the effects of Vitamins D, B9, and B12 given individually or combined in ameliorating some biochemical parameters related to endothelial dysfunction in diabetic rats.Methods: A total of 50 Sprague-Dawley male rats were divided into five groups: Control, diabetic, diabetic received Vitamin D, diabetic received Vitamins B9 and B12, and diabetic received Vitamins B9, B12, and D. At the end of 6 weeks, the rats were sacrificed and a set of assays was carried out to determine: Fasting blood sugar (FBS), lipid profile, nitric oxide (NO), homocysteine (Hcy), malondialdehyde (MDA), and serum levels of Vitamins B9, B12, and D.Results: Diabetic rat received Vitamin D and diabetic rat received Vitamins B9 and B12 had a significant decline in the levels of FBS, lipid profile, and Hcy with reduced MDA (p<0.05) release but significant increase in NO level. On the same hand, diabetic rat received combined supplementation of Vitamins B9, B12, and D had more pronounced effect (p<0.00).Conclusion: Given these findings, the combined vitamins therapy had antiatherosclerotic effects by inhibiting lipid peroxidation and stimulating NO production, resulting in amelioration the endothelial dysfunction in diabetic rat.

Vitamin D Impact on Human Health and Its Relation With Several Diseases

“…In a recent study of 2018 it was concluded that co-administration of Vitamins D, B9, and B12 exerted some antiatherosclerotic effects through inhibiting lipid peroxidation coupled with increasing aortic tissue nitric oxide (NO). These findings may have important uses and implications in the modulation and protection against diabetic endothelial dysfunction [42].…”