Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration

Abstract

Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.

Influence of platelet storage time on human platelet lysates and platelet lysate-expanded mesenchymal stromal cells for bone tissue engineering

“…Interactions between cytokines were analyzed using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database and online software [ 19 ]. Cytokines were clustered according to the Markov Cluster algorithm and the STRING global score as previously reported [ 20 ].…”

Magnesium hydroxide-incorporated PLGA composite attenuates inflammation and promotes BMP2-induced bone formation in spinal fusion

“…Control PLGA and PLGA/MH composites were placed in vials containing 1 ml of PBS solution in a shaking water bath under physiological conditions (pH 7.4, 100 rpm and 37°C). At predetermined time points (1,3,5,7,14,21,28,35,42,49, and 56 days), pH level was determined via pH-meter (Mettler Toledo, Ohio, USA). For the residual mass percentage determination, the film was cut (3 × 7 mm), initially weighted (W 0 ), immersed in PBS solution for a predetermined time, washed by deionized water, dried under vacuum for 2 days, and finally weighed (W t ).…”

“…The BMP2 loaded onto control PLGA and PLGA/MH composites was immersed in 4 M Guanidine-HCI and protease inhibitors. It followed the assay procedure and at predetermined time (1,3,5,7,14,21,28,35,42,49, and 56 days), the amount of released BMP2 was determined.…”

“…The carriers used for delivery are made of metals, ceramics, polymers, and composites. 5,[10][11][12][13][14][15][16][17][18][19] Poly(lactic-co-glycolic acid) (PLGA) has been the most successful polymeric biomaterial for use in controlled drug delivery systems. 16,18,20 Many PLGA delivery systems for BMP2 have shown promise for bone repair.…”

“…Many carriers and delivery systems comprised of different materials have been investigated to maintain the controlled release and improve the safety and therapeutic efficacy of BMP2. 5 , 10 – 16 The delivery systems come in the form of hydrogels, microspheres, nanoparticles, and fibers. The carriers used for delivery are made of metals, ceramics, polymers, and composites.…”