Efficacy of cocktail phage therapy in treating Vibrio cholerae infection in rabbit model

Jaiswal, Abhishek; Koley, Hemanta; Ghosh, Amit; Palit, A.; Sarkar, B. L.

doi:10.1016/j.micinf.2012.11.002

Cited by 58 publications

(39 citation statements)

References 16 publications

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“…In general, phage propagation on infecting bacteria was observed in cases where infections were successfully controlled by phages for example with E. coli infections of pigs and calves . Poor phage propagation correlated with ineffective control of infection in animals and in humans …”

Section: Entering the Systemmentioning

confidence: 99%

Phage therapy: What factors shape phage pharmacokinetics and bioavailability? Systematic and critical review

Dąbrowska

2019

Medicinal Research Reviews

199

212

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Bacteriophages are not forgotten viruses anymore: scientists and practitioners seek to understand phage pharmacokinetics in animals and humans, investigating bacteriophages as therapeutics, nanocarriers or microbiome components. This review provides a comprehensive overview of factors that determine phage circulation, penetration, and clearance, and that in consequence determine phage applicability for medicine. It makes use of experimental data collected by the phage community so far (PubMed 1924‐2016, including non‐English reports), combining elements of critical and systematic review. This study covers phage ability to enter a system by various routes of administration, how (and if) the phage may access various tissues and organs, and finally what mechanisms determine the courses of phage clearance. The systematic review method was applied to analyze (i) phage survival in the gut (gut transit) and (ii) phage ability to enter the mammalian system by many administration routes. Aspects that have not yet been covered by a sufficient number of reports for mathematical analysis, as well as mechanisms underlying trends, are discussed in the form of a critical review. In spite of the extraordinary diversity of bacteriophages and possible phage applications, the analysis revealed that phage morphology, phage specificity, phage dose, presence of sensitive bacteria or the characteristics of treated individuals (age, taxonomy) may affect phage bioavailability in animals and humans. However, once phages successfully enter the body, they reach most organs, including the central nervous system. Bacteriophages are cleared mainly by the immune system: innate immunity removes phages even when no specific response to bacteriophages has yet developed.

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Section: Entering the Systemmentioning

confidence: 99%

Phage therapy: What factors shape phage pharmacokinetics and bioavailability? Systematic and critical review

Dąbrowska

2019

Medicinal Research Reviews

199

212

View full text Add to dashboard Cite

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“…The combination of two or more phages with different host ranges in a single suspension-a phage cocktail-has been reported to be more effective than the use of a single phage alone (63)(64)(65). Accordingly, Hall et al studied the effect of using one, two, or four phages, either sequentially or simultaneously, against P. aeruginosa PAO1 planktonic cultures (66).…”

Section: In Vitro Phage Trialsmentioning

confidence: 99%

Phage Therapy: a Step Forward in the Treatment of Pseudomonas aeruginosa Infections

Pires

Boas

Sillankorva

et al. 2015

J Virol

137

103

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Antimicrobial resistance constitutes one of the major worldwide public health concerns. Bacteria are becoming resistant to the vast majority of antibiotics, and nowadays, a common infection can be fatal. To address this situation, the use of phages for the treatment of bacterial infections has been extensively studied as an alternative therapeutic strategy. Since Pseudomonas aeruginosa is one of the most common causes of health care-associated infections, many studies have reported the in vitro and in vivo antibacterial efficacy of phage therapy against this bacterium. This review collects data of all the P. aeruginosa phages sequenced to date, providing a better understanding about their biodiversity. This review further addresses the in vitro and in vivo results obtained by using phages to treat or prevent P. aeruginosa infections as well as the major hurdles associated with this therapy.

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“…A combination of phages with different host ranges in a single cocktail is currently the most common approach taken to achieve a wider target spectrum, because a single therapeutic mixture can be applied to a broader range of bacterial infections than a single phage alone. Although this strategy is promising (94,(126)(127)(128), it remains difficult to target all bacterial strains in a given species, and the diverse assortment of phages per cocktail may unintentionally target bacteria outside the desired range. This is a challenge with both natural and current-generation engineered phages.…”

Section: Engineered Phages With Shifted or Broadened Host Rangesmentioning

confidence: 99%

Genetically Engineered Phages: a Review of Advances over the Last Decade

Pires

Cleto

Sillankorva

et al. 2016

Microbiol Mol Biol Rev

315

275

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SUMMARYSoon after their discovery in the early 20th century, bacteriophages were recognized to have great potential as antimicrobial agents, a potential that has yet to be fully realized. The nascent field of phage therapy was adversely affected by inadequately controlled trials and the discovery of antibiotics. Although the study of phages as anti-infective agents slowed, phages played an important role in the development of molecular biology. In recent years, the increase in multidrug-resistant bacteria has renewed interest in the use of phages as antimicrobial agents. With the wide array of possibilities offered by genetic engineering, these bacterial viruses are being modified to precisely control and detect bacteria and to serve as new sources of antibacterials. In applications that go beyond their antimicrobial activity, phages are also being developed as vehicles for drug delivery and vaccines, as well as for the assembly of new materials. This review highlights advances in techniques used to engineer phages for all of these purposes and discusses existing challenges and opportunities for future work.

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Efficacy of cocktail phage therapy in treating Vibrio cholerae infection in rabbit model

Cited by 58 publications

References 16 publications

Phage therapy: What factors shape phage pharmacokinetics and bioavailability? Systematic and critical review

Phage therapy: What factors shape phage pharmacokinetics and bioavailability? Systematic and critical review

Phage Therapy: a Step Forward in the Treatment of Pseudomonas aeruginosa Infections

Genetically Engineered Phages: a Review of Advances over the Last Decade

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