Efficacy of cavity liners with/without atmospheric cold helium plasma jet for dentin remineralization

Abstract

Aim: This study aimed to assess the efficacy of cavity liners with/without the atmospheric cold plasma jet (ACPJ) for dentin remineralization. Material and methods: The occlusal third of 24 extracted third molars was cut. An occlusal cavity was prepared in the dentin of each tooth with 1 mm depth and 2 mm diameter and demineralized with 37% phosphoric acid for 1 min. The teeth were randomly divided into 8 groups (n ¼ 3). The first control group only underwent demineralization with phosphoric acid. The second control group underwent demineralization and helium plasma jet. Groups 3 to 5 were filled with calcium hydroxide (CH), RetroMTA (MTA) and Biodentine. Groups 6 to 8 were subjected to ACPJ, and all the groups were sealed with polycarboxylate. After 2 weeks of immersion in water, the teeth were longitudinally sectioned and their mineral content was analyzed using energy-dispersive X-ray spectroscopy (EDX). Results: The interaction effect of ACPJ and type of liner was not significant (p > 0.05). Application of ACPJ in combination with liner had a significant effect on calcium and phosphorous contents (p < .05). The calcium and phosphorous contents in the control groups were significantly lower than other groups (p < .05). The calcium and phosphorous contents in the CH group were higher than the control but significantly lower than the MTA and Biodentine groups. The values in the MTA and Biodentine groups were the same and higher than other groups. Conlusion: All three cavity liners significantly increased the calcium and phosphorous contents of dentin. This increase was significantly greater by the MTA and Biodentine and enhanced by the ACPJ.

Effect of Cold Atmospheric Pressure Plasma Coupled with Resin-Containing and Xylitol-Containing Fluoride Varnishes on Enamel Erosion

“…Helium has a lower ionization rate than argon and better thermal conductivity, preventing thermal instability [ 37 ]. Furthermore, surface treatment with plasma jet has been suggested to enhance recrystallization of minerals [ 26 ]. It can be used to produce nano-scale crystals with high quality and improve the dissolution speed [ 38 , 39 ].…”

“…The soft tissue residues were removed using a gauze, and calculus was removed using a hand scaler. The teeth were then disinfected by immersion in 0.5% chloramine T solution for 1 week [ 26 ]. Then, the teeth were decoronated at 3 mm below the cementoenamel junction by a diamond metal disc (North Bel Co., Paderno Dugnano, Italy).…”

The effect of argon cold atmospheric plasma on the metabolism and demineralization of oral plaque biofilms

“…In this part of the experiment plaque, biofilms were precultured on the enamel surface and the above biofilm culture process has been treated with this plasma to simulate the caries demineralization process in vitro . The CAP treatment leads to changes in the surface roughness and morphology of the hard tissues of the teeth and results in a reduction in the contact angle between water and glycol, as well as a significant reduction in carbon on the enamel surface, all of which have an effect on the mineralization and remineralization of the hard tissues of the teeth ( Chen et al., 2013 ; Kermanshah et al., 2020 ).…”

Applications of atmospheric cold plasma in agricultural, medical, and bioprocessing industries

“…In dentistry, ACP has currently been applied in several processes including instrument sterilization (Sung et al 2013 ), dental caries (Kermanshah et al 2020 ), root canal infection (Armand et al 2019 ; Wen et al 2022 ), and tooth bleaching (Pavelić et al 2020 ).…”