Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of<i> Aedes aegypti</i> (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka

Gunathilaka, Nayana; Ranathunge, Tharaka; Udayanga, N.W.B.A.L.; Abeyewickreme, W.

doi:10.1155/2017/3196924

Cited by 62 publications

(76 citation statements)

References 18 publications

(18 reference statements)

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“…3 Sin embargo, de acuerdo con la literatura disponible, las hembras Aedes aegypti optan por sangre de mamíferos huéspedes con preferencia por la sangre humana en varias ocasiones durante su ciclo gonotrófico, inclusive en presencia de hospederos alternativos. 14,15 por lo que en este estudio la sangre humana se empleó como un control.…”

Section: Discussionunclassified

Eficacia de diferentes fuentes de alimentación sanguínea sobre Aedes aegypti (Diptera: Culicidae) en condiciones de insectario

Fernández¹,

Anzúrez²,

Medrano³

et al. 2019

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Objetivo. Evaluar el efecto de tres fuentes de alimentación sanguínea: conejo Nueva Zelanda, rata egipcia y humana, sobre el número de huevos colocados por ovipuesta y porcentaje de eclosión de las larvas Aedes aegypti en condiciones de insectario. Material y métodos: Grupo de mosquitos hembra Aedes aegypti con 1 día post-emergencia fueron alimentadas en condiciones de insectario con tres diferentes fuentes sanguíneas. Los grupos alimentados con conejo Nueva Zelanda y rata egipcia se expusieron de forma directa dentro de las jaulas de emergencia con los mosquitos; y en los grupos de mosquitos alimentados con la fuente sanguínea humana se utilizó una membrana artificial de algodón. Los datos fueron analizados con una prueba no paramétrica de Kruskal-wallis (p ≤ 0.05). Resultados: Se observó una diferencia estadísticamente significativa en el promedio de huevos colocados entre los tres grupos de hembras Aedes aegypti alimentados con diferente fuentes sanguíneas: sangre de rata, 671.25 huevos; sangre humana, 268.14 huevos y sangre de conejo, 209.08 huevos (X2= 10.666, P < 0.0048); el porcentaje de larvas eclosionadas no presentó diferencias estadísticamente significativas entre las tres fuentes sanguíneas (X2= 0.192, p < 0.9083). Conclusiones: En este estudió se observó que el uso de sangre de rata egipcia como fuente de alimentación sanguínea para la producción de mosquitos Aedes aegypti en condiciones de insectario produce un mayor número de huevos en la ovipostura comparado con la sangre de conejo y de humano, sin embargo, es necesario realizar más evaluaciones para identificar si ésta fuente de sangre garantiza un alto porcentaje de eclosión con respecto a la sangre humana y de conejo, así como evaluar las diferentes técnicas de alimentación artificial con sangre humana.

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Section: Discussionunclassified

Eficacia de diferentes fuentes de alimentación sanguínea sobre Aedes aegypti (Diptera: Culicidae) en condiciones de insectario

Fernández¹,

Anzúrez²,

Medrano³

et al. 2019

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“…Adult mosquitoes were collected periodically with a mouth aspirator and transferred to the rearing cage. Females were fed with fresh chicken blood using a Hemotek © blood feeding system (54).…”

Section: Methodsmentioning

confidence: 99%

Volatile allosteric antagonists of mosquito odorant receptors inhibit normal odor-dependent behaviors

Kythreoti

Sdralia

Tsitoura

et al. 2020

Preprint

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Odorant-dependent behaviors in insects are triggered by the binding of odorant ligands to the variable subunits of heteromeric olfactory receptors. Previous studies have shown, however, that specific odor binding to ORco, the common subunit of odorant receptor heteromers, may alter allosterically olfactory receptor function and affect profoundly subsequent behavioral responses. Here we report on the identification of several antagonists of the odorant receptor co-receptor of the African malaria vector Anopheles gambiae, AgamORco, in a small collection of natural volatile organic compounds (VOCs) using a relevant insect cell-based screening platform. Because some of the identified antagonists were previously shown to strongly repel Anopheles and Culex mosquitoes, here we examined the bioactivities of the identified antagonists against Aedes, the third major genus of the Culicidae family. The tested antagonists were found to inhibit the function of Ae. aegypti ORco ex vivo and repel Asian tiger, Ae. albopictus, adult mosquitoes. Specific antagonist binary mixtures elicited higher repellency than single antagonists. Binding competition assays suggested antagonist binding to distinct ORco sites as a likely cause for the enhanced repellence of the blends. These findings demonstrate that a simple screening assay may be used for the identification of allosteric modifiers of olfactory-driven 2 behaviors capable of providing enhanced indoor and outdoor protection against multiple mosquito borne infectious diseases.

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“…Females of the mosquito colonies emerged from different larval diet concentrations were fed with cattle blood meal artificially by metal plate method as described by Gunathilaka et al (2017).…”

Section: Methodsmentioning

confidence: 99%

Determination of the efficiency of diets for larval development in mass rearing Aedes aegypti (Diptera: Culicidae)

Gunathilaka

Uduwawala

Udayanga

et al. 2017

Bull. Entomol. Res.

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Larval diet quality and rearing conditions have a direct and irreversible effect on adult traits. Therefore, the current study was carried out to optimize the larval diet for mass rearing of Aedes aegypti, for Sterile Insect Technique (SIT)-based applications in Sri Lanka. Five batches of 750 first instar larvae (L1) of Ae. aegypti were exposed to five different concentrations (2-10%) of International Atomic Energy Agency (IAEA) recommended the larval diet. Morphological development parameters of larva, pupa, and adult were detected at 24 h intervals along with selected growth parameters. Each experiment was replicated five times. General Linear Modeling along with Pearson's correlation analysis were used for statistical treatments. Significant differences (P < 0.05) among the larvae treated with different concentrations were found using General Linear Modeling in all the stages namely: total body length and the thoracic length of larvae; cephalothoracic length and width of pupae; thoracic length, thoracic width, abdominal length and the wing length of adults; along with pupation rate and success, sex ratio, adult success, fecundity and hatching rate of Ae. aegypti. The best quality adults can be produced at larval diet concentration of 10%. However, the 8% larval diet concentration was most suitable for adult male survival.

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Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka

Cited by 62 publications

References 18 publications

Eficacia de diferentes fuentes de alimentación sanguínea sobre Aedes aegypti (Diptera: Culicidae) en condiciones de insectario

Eficacia de diferentes fuentes de alimentación sanguínea sobre Aedes aegypti (Diptera: Culicidae) en condiciones de insectario

Volatile allosteric antagonists of mosquito odorant receptors inhibit normal odor-dependent behaviors

Determination of the efficiency of diets for larval development in mass rearing Aedes aegypti (Diptera: Culicidae)

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