Efficacy of biological preparations of soybean root nodule bacteria modified with a homologous lectin

Сытников, Д. М.; Kots, S.Yа.; Datsenko, V. K.

doi:10.1134/s0003683807030076

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…Our studies also show the effectiveness of biological products of nodule bacteria modified by homologous lectin, and the economic feasibility of their use [47,[49][50][51][77][78][79]. In accordance with the existing concepts about the mechanisms of interaction of plants with rhizobia, polysaccharides of the latter are a factor which provides a "recognition" by the bacteria of the corresponding host plant through complementary binding to the plant lectin.…”

Section: Ways To Improve the Productivity Of The Soybean-rhizobial Sysupporting

confidence: 63%

“…This is why in recent years work was done on the selection of the optimal concentration of the homologous lectin, and the length of time of its co-incubation with the culture of Bradyrhizobium japonicum in the production of bacterial fertilizers both in liquid and solid form. Lectin is used as a growth stimulating biologically active substance, which is introduced into the culture of bacteria under certain conditions [51].…”

Section: Legume Lectins As a Factor In An Effective Symbiosismentioning

confidence: 99%

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How to Increase the Productivity of the Soybean-Rhizobial Symbiosis

Сытников¹

2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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Section: Ways To Improve the Productivity Of The Soybean-rhizobial Sysupporting

confidence: 63%

Section: Legume Lectins As a Factor In An Effective Symbiosismentioning

confidence: 99%

How to Increase the Productivity of the Soybean-Rhizobial Symbiosis

Сытников¹

2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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“…Lectins can interact with bacterial polysaccharides, mainly lipopolysaccharides (LPS), to promote bacterial adhesion and improve N 2 fixation [7,8]. Lectins also stimulate cellular respiration, induce bacterial growth and modulate metabolism [9,10,11,12]. The lectins ConA and ConBr were isolated from the seeds of two species of the Diocleinae subtribe, Canavalia ensiformis and Canavalia brasiliensis , respectively.…”

Section: Introductionmentioning

confidence: 99%

Lectin from Canavalia brasiliensis Seeds (ConBr) Is a Valuable Biotechnological Tool to Stimulate the Growth of Rhizobium tropici in Vitro

et al. 2012

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Abstract:To study the interactions between a Rhizobium tropici strain and lectins isolated from the seeds of Canavalia ensiformis (ConA) and Canavalia brasiliensis (ConBr), a lectin fluorescence assay was performed. In addition, an experiment was designed to evaluate the effect of the two lectins on bacterial growth. Both lectins were found to bind to R. tropici cells, but the interactions were inhibited by D-mannose. Interestingly, only ConBr stimulated bacterial growth in proportion to the concentrations used (15.6-500 µg/mL), and the bacterial growth stimulation was inhibited by D-mannose as well. Structure/Function OPEN ACCESSMolecules 2012, 17 5245 analyses by bioinformatics were carried out to evaluate the volume and carbohydrate recognition domain (CRD) configuration of ConA and ConBr. The difference of spatial arrangement and volume of CRD may indicate the variation between biological activities of both lectins. The results suggest that ConBr could be a promising tool for studies focusing on the interactions between rhizobia and host plants.

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“…Some studies showed that lectins may interact with rhizobia and modulate its metabolism, inducing the protein synthesis [ 14 ], increasing cellular respiration [ 15 ], stimulating the efflux of H + [ 16 ] and promoting bacterial growth [ 17 , 18 ]. Moreover, the use of lectins can enhance the symbiotic efficiency between rhizobia and host plant, leading to an improvement in plant productivity [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899

et al. 2013

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Rhizobium tropici is a Gram-negative bacterium that induces nodules and fixed atmospheric nitrogen in symbiotic association with Phaseolus vulgaris (common bean) and some other leguminous species. Lectins are proteins that specifically bind to carbohydrates and, consequently, modulate different biological functions. In this study, the d-glucose/d-mannose-binding lectins (from seeds of Dioclea megacarpa, D. rostrata and D. violacea) and d-galactose-binding lectins (from seeds of Bauhinia variegata, Erythina velutina and Vatairea macrocarpa) were purified using chromatographic techniques and evaluated for their effect on the growth of R. tropici CIAT899. All lectins were assayed with a satisfactory degree of purity according to SDS-PAGE analysis, and stimulated bacterial growth; in particular, the Dioclea rostrata lectin was the most active among all tested proteins. As confirmed in the present study, both d-galactose- and d-glucose/d-mannose-binding lectins purified from the seeds of leguminous plants may be powerful biotechnological tools to stimulate the growth of R. tropici CIAT99, thus improving symbiotic interaction between rhizobia and common bean and, hence, the production of this field crop.

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Efficacy of biological preparations of soybean root nodule bacteria modified with a homologous lectin

Cited by 5 publications

References 6 publications

How to Increase the Productivity of the Soybean-Rhizobial Symbiosis

How to Increase the Productivity of the Soybean-Rhizobial Symbiosis

Lectin from Canavalia brasiliensis Seeds (ConBr) Is a Valuable Biotechnological Tool to Stimulate the Growth of Rhizobium tropici in Vitro

Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899

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