Efficacy of Baculoviruses and their Impact on Pest Management Programs

Funderburk, Joe; Maruniak, James E.; Boucias, Drion; Garcia-Canedo, Alejandra

doi:10.1007/978-94-011-2870-4_8

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Anticarsia gemmatalis Hübner é uma praga que causa sérios danos à cultura da soja na América Latina e sudeste dos Estados Unidos (Funderburk et al, 1992;Tanada & Kaya, 1993). A ocorrência de um NPV nesta espécie foi notificada pela primeira vez em insetos coletados em alfafa, no Peru (Steinhaus & Marsh, 1962).…”

Section: Baculovírus Usados Para O Controle Biológico De Insetos No Bunclassified

“…Devido a sua alta especificidade e ocorrência natural, os baculovírus são ótimos candidatos a serem usados em programas de manejo integrado de pragas (Moscardi, 1990;Funderburk et al, 1992;Tanada & Kaya, 1993), pois em diversos testes de segurança foi estabelecido que o vírus é inofensivo a microrganismos, outros invertebrados (exceto alguns insetos), vertebrados e plantas (Payne,1986;Gröner, 1989).…”

Section: Introductionunclassified

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Molecular biology of baculovirus and its use in biological control in Brazil

Castro

Souza

Sihler

et al. 1999

Pesq. agropec. bras.

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4RESUMO -Os baculovírus são vírus patogênicos a insetos, encontrados principalmente na ordem Lepidoptera. A família Baculoviridae é taxonomicamente dividida em dois gêneros: Nucleopolyhedrovirus e Granulovirus, que diferem pela morfologia do corpo de oclusão. Os nucleopoliedrovírus (NPV) possuem corpos de inclusão poliédrica (PIB), contendo múltiplas partículas virais, enquanto os granulovírus (GV) contêm, em geral, partículas únicas, ocluídas em corpos protéicos de forma ovóide. Durante o ciclo de vida são produzidos dois tipos de progênies virais: BV (Budded Virus) e PDV (Polyhedra Derived Virus), que são essenciais para o processo de infecção e propagação do vírus. Os baculovírus têm sido empregados no controle de pragas e, por serem específicos e restritos a invertebrados, são considerados agentes seguros de controle biológico. Recentemente têm sido amplamente utilizados como vetor de expressão de genes heterólogos por produzirem e processarem, em grande quantidade, proteínas de procariotos e eucariotos. Além disso, técnicas de DNA recombinante têm permitido a produção de inseticidas virais geneticamente modificados. Este trabalho constitui uma revisão sobre a taxonomia, estrutura, replicação e biologia molecular de baculovírus e sobre seu uso como bioinseticida no Brasil.

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Section: Baculovírus Usados Para O Controle Biológico De Insetos No Bunclassified

Section: Introductionunclassified

Molecular biology of baculovirus and its use in biological control in Brazil

Castro

Souza

Sihler

et al. 1999

Pesq. agropec. bras.

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Physical maps and virulence of Anticarsia gemmatalis nucleopolyhedrovirus genomic variants

et al. 1999

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Seventeen plaque purified isolates of two viral preparations of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), were analyzed in terms of the genomic changes after digestion of their DNAs with HindIII and PstI restriction enzymes. The 1979 AgMNPV wild type preparation (AgMNPV-'79) resulted in six different variants and the 1985 viral commercial preparation (AgMNPV-'85), in eleven. The genomic variation of all the isolates was mapped showing that those from 1985 presented more heterogeneity with changes mapped in additional sites in comparison to the AgMNPV-'79 variants. Their virulence was compared by infecting two Lepidopteran cell lines, Spodoptera frugiperda (IPLB-SF-21AE) and Anticarsia gemmatalis (UFL-AG-286). The results indicated that there was some difference in virulence within the AgMNPV-'85 variants. This commercial preparation had been applied in soybean fields in Brazil over several years to control the velvetbean caterpillar defoliation.

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Assessment of the Application of Baculoviruses for Control of Lepidoptera

Moscardi

1999

Annu. Rev. Entomol.

737

478

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Baculoviruses, among other insect viruses, are regarded as safe and selective bioinsecticides, restricted to invertebrates. They have been used worldwide against many insect pests, mainly Lepidoptera. Their application as microbial pesticides, however, has not met their potential to control pests in crops, forests, and pastures, with the exception of the nuclear polyhedrosis virus of the soybean caterpillar (Anticarsia gemmatalis), which is used on approximately 1 million ha annually in Brazil. Problems that have limited expansion of baculovirus use include narrow host range, slow killing speed, technical and economical difficulties for in vitro commercial production, timing of application based on frequent host population monitoring, variability of field efficacy due to climatic conditions, and farmers' attitudes toward pest control, which have been based on application of fast-killing chemical insecticides. Farmer education regarding use of biological insecticides and their characteristics is considered one of the major actions necessary for increased use of baculoviruses. Strategies to counteract some of the limitations of baculoviruses, especially their slow killing activity, have been investigated and are promising. These include the use of chemical or biological substances added to virus formulations and genetic engineering of the viruses themselves to express insect toxins or hormones. Such strategies can enhance viral activity and increase speed of kill as well as reduce larval feeding activity. The use of baculoviruses against Lepidoptera is reviewed, with the utilization of the nuclear polyhedrosis virus of A. gemmatalis in Brazil serving as a case-study.

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Efficacy of Baculoviruses and their Impact on Pest Management Programs

Cited by 3 publications

References 13 publications

Molecular biology of baculovirus and its use in biological control in Brazil

Molecular biology of baculovirus and its use in biological control in Brazil

Physical maps and virulence of Anticarsia gemmatalis nucleopolyhedrovirus genomic variants

Assessment of the Application of Baculoviruses for Control of Lepidoptera

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