Efficacy of Bacteriophage Therapy on Horizontal Transmission of Salmonella Gallinarum on Commercial Layer Chickens

Lim, Tae-Hyun; Lee, Dong Hun; Lee, Yuna; Park, Jaekeun; Youn, Ha-Na; Kim, Myung-Seob; Lee, Hyun-Jeong; Yang, Si-Yong; Cho, Young Wook; Lee, Joong‐Bok; Park, Seung‐Yong; Choi, In‐Soo; Song, Chang‐Seon

doi:10.1637/9599-111210-reg.1

Cited by 32 publications

(13 citation statements)

References 19 publications

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“…The observation that the Gallinarum and Pullorum genomes are relatively free of prophages suggests that the fowl-specific Salmonella may be sensitive to more phages compared to Enteritidis and Dublin. Such phages would be useful in cocktails for use in prophylactic phage therapy, a rekindled approach to control Salmonella infections in poultry houses [ 78 – 80 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum

et al. 2015

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The Salmonella enterica serovars Enteritidis, Dublin, and Gallinarum are closely related but differ in virulence and host range. To identify the genetic elements responsible for these differences and to better understand how these serovars are evolving, we sequenced the genomes of Enteritidis strain LK5 and Dublin strain SARB12 and compared these genomes to the publicly available Enteritidis P125109, Dublin CT 02021853 and Dublin SD3246 genome sequences. We also compared the publicly available Gallinarum genome sequences from biotype Gallinarum 287/91 and Pullorum RKS5078. Using bioinformatic approaches, we identified single nucleotide polymorphisms, insertions, deletions, and differences in prophage and pseudogene content between strains belonging to the same serovar. Through our analysis we also identified several prophage cargo genes and pseudogenes that affect virulence and may contribute to a host-specific, systemic lifestyle. These results strongly argue that the Enteritidis, Dublin and Gallinarum serovars of Salmonella enterica evolve by acquiring new genes through horizontal gene transfer, followed by the formation of pseudogenes. The loss of genes necessary for a gastrointestinal lifestyle ultimately leads to a systemic lifestyle and niche exclusion in the host-specific serovars.

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Section: Discussionmentioning

confidence: 99%

Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum

et al. 2015

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“…The antibacterial properties of phages have found application in experimental therapies in humans and animals and in the development of disinfectants eliminating bacteria from the surfaces of foods of plant and animal origin (Abuladze et al, 2008;Carlton, Noordman, Biswas, Meester, & Loessner, 2005;Lim et al, 2011;Weber-Dąbrowska, Mulczyk, & Górski, 2000).…”

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confidence: 99%

Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages

et al. 2019

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Bacteria of the genus Campylobacter are the most common pathogens causing zoonotic diseases in humans. Therefore, the aim of the study was to isolate Campylobacter bacteria from broiler chickens and evaluate their susceptibility to selected antibiotics by determining minimum inhibitory concentrations (MIC), followed by isolation and characterization of bacteriophages specific for Campylobacter spp. The material for the study consisted of field isolates of Campylobacter spp. obtained from the gut (cecum) of broiler chickens directly after slaughter in slaughterhouses, and bacteriophages specific for these strains. We isolated 48 strains from poultry (140 broiler chickens): 31 strains of Campylobacter jejuni and 17 of Campylobacter coli. Identification of the strains was confirmed by multiplex PCR and MALDI‐TOF mass spectrometry. Over 83% of Campylobacter strains were resistant to ciprofloxacin, and over half the isolates were resistant to erythromycin, gentamicin, and tetracycline. Resistance to three or more antibiotics was observed in 91.6% of all strains. Four bacteriophages were obtained, and on the basis of their morphological structure, they were assigned to two families of the order Caudovirales : Myoviridae and Siphoviridae . A high percentage of the Campylobacter strains were resistant to at least three of the antibiotic groups tested. All of the phages exhibited lytic activity against the Campylobacter spp. isolates, but the antibacterial effect of the phages was not observed for all strains.

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“…Bir baflka çal›flmada, tavuklarda Salmonella bakterisinin kümes içerisinde bulaflmas›n› önlemek amac›yla yemlerle birlikte faj uygulamas›n›n etkisi incelenmifltir. Çal›flmada faj uygulamas›n›n Salmonella'n›n kümes içerisinde yay›lmas›n› önledi¤i rapor edilmifltir (46). Bakteriyofajlar›n biyokontrol ajanlar› olarak kullan›m› ile ilgili olarak yap›lan çal›flmalarda, E.coli O157:H7 say›s›n› düflürücü etkisiyle fajlar oldukça etkili sonuçlar ortaya koymufltur (47,48,49).…”

Section: Bakteri̇yofaj Enkapsülasyonu Potansi̇yel Uygulamalariunclassified

Bakteri̇yofaj Enkapsülasyonu Ve Potansi̇yel Uygulamalari

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2017

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ÖzetEnkapsülasyon, aktif olan maddenin çevresinde uygun kaplama materyali ile koruyucu bir zar oluflturulmas› temeline dayanmaktad›r. G›da endüstrisinde mikroenkapsülasyon istenen bileflenin d›fl etkenlere karfl› korunmas›, baz› özelliklerinin sürdürülebilmesi, kolay tafl›nmas›, tat ve koku maskeleme, oluflabilecek reaksiyonlar›n önüne geçme gibi amaçlarla kullan›lmas›na ra¤men koruyucu ve tedavi amaçl› fajlar›n enkapsülasyonunun da oldukça etkili bir yöntem olarak uygulanabildi¤i bilinmektedir. Bakteriyofajlar 20. yüzy›l›n bafllar›ndan beri bakteriyel enfeksiyonlara karfl› koruma ve tedavi amac›yla kullan›lm›fl ancak artan antibiyotik tüketimi, fajlar›n tedavi amaçl› kullan›m›n›n önüne geçmifltir. En önemli özelli¤i yüksek spesifitesi olan bakteriyofajlar, çiftlik hayvanlar›nda patojen kolonizasyonunun azalt›lmas› (faj tedavisi), çi¤ süt, et ve taze g›dalarda dekontaminasyon (biyokontrol), ekipman yüzeylerinde sanitasyon (biyosanitasyon), haz›r g›dalarda raf ömrü uzatmak (biyoprezervasyon) amac›yla uygulanabilen etkin bir yöntem olarak karfl›m›za ç›kmaktad›r. Bakteriyofaj enkapsülasyon uygulamalar›nda, fonksiyonel kaplama uygulamalar›ndaki geliflmelere paralel olarak daha uygun maliyetli ve daha uzun süre muhafaza edilebilen mikrokapsül teknolojilerinin gelifltirilmesine ihtiyaç duyulmaktad›r.Anahtar kelimeler: Enkapsülasyon, bakteriyofaj, faj tedavi, biyokontrol, biyosanitasyon, biyoprezervasyon BACTERIOPHAGE ENCAPSULATION AND POTENTIAL APPLICATIONS AbstractEncapsulation is based on forming a protective membrane around the active substance with a suitable coating material. Although microencapsulation is applied for purposes including protection of some components against external factors, preservation of some features, easy handling, flavor and odor masking, prevention of some reactions, it is known that the phage encapsulation can be highly effective for protection and treatment purposes. Since the early 20th century, bacteriophages are used for the prevention and treatment of bacterial infections, however the increase in the consumption of antibiotics has prevented the use of phages for treatment. One of the most important properties of bacteriophages is the high specificity. They are effectively used for the reduction of pathogen colonization in farm animals (phage therapy), decontamination of raw milk, meat and fresh food (biocontrol), sanitation of equipment surfaces (biosanitation), extending the shelf life of prepared foods (biopreservation). There is a need for the development of microcapsule technology which is relatively cost effective and allows longer storage periods in parallel with the developments in functional coating applications.

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Efficacy of Bacteriophage Therapy on Horizontal Transmission of Salmonella Gallinarum on Commercial Layer Chickens

Cited by 32 publications

References 19 publications

Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum

Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum

Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages

Bakteri̇yofaj Enkapsülasyonu Ve Potansi̇yel Uygulamalari

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