Efficacy of Bacterial Isolates against Causal Agent of Late Blight of Potato, Phytophthora infestans

Abstract

Background: Potato (Solanum tuberosum) is main crop in subtropical and temperate regions as vegetable crop. It is affected by Phytophthora infestans causing a devasting disease late blight. The chemical pesticides used against this disease in this present scenario cause pollution (soil as well as water) and adversely affect beneficial soil micro-flora, grazing animals and human beings. Methods: An experiment was designed at the Department of Botany, K.V. Subharti College of Science, Swami Vivekanand Subharti University, Meerut (UP), India during 2017-2018. Three bacterial bioagents, Pseudomonas isolate 1 and 2 and Bacillus species was isolated from infected phylloplane and used against late blight pathogen, P. infestans. The efficacy of antagonistic bio-agents tested against mycelial growth, zoospore and zoosporangial germination of P. infestans by dual culture method and detached leaf method.Result: The present study focused on use of bio-agents or antagonistic bacteria to control late blight pathogen showed concentration biased potential inhibitory activity against mycelial growth, zoospore and zoosporangial germination. It means higher the concentration of bacterial isolate, greater the inhibitory effect on various tested parameters. Among the three tested antagonistic bioagents, Bacillus showed maximum zone of inhibition to late blight pathogen. The proved that the Pseudomaonas and Bacillus as potential antagonistic agent for ecofriendly sustainable management of pathogenic mycoflora, P. infestans.

Effect of Plant Growth Promoting Bacillus spp. on Germination and Seedling Growth of Soybean

“…cepae, F. proliferatum, F. verticillioides and F. acuminatum, isolated from infected garlic cloves (Bjelić et al, 2018). Similarly, Rani et al (2021) observed that Bacillus showed Effect of Plant Growth Promoting Bacillus spp. on Germination and Seedling Growth of Soybean maximum zone of inhibition to the tested pathogen among the three antagonistic biocontrol agents.…”

The potential of rhizobacteria to control soil-borne fungal pathogens in tomato

“…Dünya çapında tarımsal ürün yetiştiriciliğinde, toprak kaynaklı bitki patojenlerinin üretimde ve verimde ciddi kayıplara neden olması yüksek ürün maliyetlerine yol açmaktadır (Panth et al, 2020). Rizosferdeki mikrobiyal aktivite ise hem doğal hem de yönetilen tarımsal topraklarda patojenlerin girişine çok yatkın olan köklerde hastalığa neden olan toprak kaynaklı bitki patojenlerini baskılanmasında kilit rol oynamaktadır (Hariprasad & Umesha, 2007;Rani et al, 2007). Bu kapsamda domates bitkisinde hastalığa neden olan toprak kökenli funguslara karşı, domates bitkilerin rizosferinden izole edilen rizobakterilerin biyokontrol aktivitelerinin belirlenmesi bu çalışmada amaçlanmıştır.…”

Screening and efficacy evaluation of antagonistic fungi against Phytophthora infestans and combination with arbuscular mycorrhizal fungi for biocontrol of late blight in potato

“…Whereas literature has amply documented the biocontrol of late blight of potato using plant or bacterial extracts (Quintanilla et al, 2002;Stephan et al, 2005;Olanya and Larkin, 2006;Dorn et al, 2007;Wang et al, 2007;Hultberg et al, 2010;Moushib et al, 2013;Bengtsson et al, 2015;Hunziker et al, 2015;Rogozhin et al, 2020;Rani et al, 2021), fewer studies have reported biocontrol of potato late blight and control of the pathogen growth with metabolites extracted from fungi. For instance, the application of oosporein, an antifungal metabolite isolated from Verticillium psalliotae, fusaric acid from F. oxysporum, and culture extracts from Trichoderma and Chaetomium species (i.e., C. cochliodes and C. aureum) have been reported to affect the growth of P. infestans in vitro (Nagaoka et al, 2004;Son et al, 2008;Bae et al, 2016;Linkies et al, 2021).…”