Efficacy of Bacillus thuringiensis israelensis (Bti) and four plant extracts on the mortality and development of Culex quinquefasciatus Say (Diptera: Cullicidae)

Gad, Abir A.; Al-Dakhil, Abeer A.

doi:10.1186/s41938-018-0066-8

Cited by 4 publications

(3 citation statements)

References 9 publications

(9 reference statements)

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“…They tested a new isolate of Bti, called LFB-Fiocruz. The LC50 values of the tested formulation against Aedes aegypti larvae were 0.0417mg/L .Some biological properties such as pupation percent, malformation, and adult emergence of dipteran insects after being exposed to B.t., were studied by (Gad & Al-Dakhil, 2018& Zaki et al, 2020. Their results agreed with the results of the current research results.…”

Section: Discussionmentioning

confidence: 99%

Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

Abeer

2022

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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Flies and mosquitoes are public health threats by transmitting various human diseases. Culex and Aedes species are insect vectors of several diseases such as malaria and dengue fever (DF). DF is a human arbovirus disease transmitted by the female mosquito of the genus Aedes, mainly Aedes aegypti, which is the most serious mosquito-borne viral disease worldwide and endemic in certain cities of Saudi Arabia, such as Jazan area, (Alhaeli et al., 2016). And because of the climate of this area enhances the breeding of mosquitoes, especially during rainfall and high humidity. Another factor is the water storage in containers that served as natural habitats for mosquitoes, (Elisa et al., 2014). Chemical-insecticides used in pest control programs, are toxic for both humans and the environment, also, many insect species gained resistance to the insecticides. Therefore, there is a need to use effective and safe alternative methods to eliminate pests. According to (Boisvert, 2007), Bacillus thuringiensis subsp israelensis was isolated for the first time in 1976 and found to be toxic to mosquito larvae. B.t.t. has entomopathogenic effects because of the crystal proteins produced during the sporulation stage. The crystal proteins paralyze the microvilli of the insect's digestive tract which leads to death by septicemia (Angelo et al., 2010). The application of bio-control agents against insects that carry human pathogens is considered

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Section: Discussionmentioning

confidence: 99%

Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

Abeer

2022

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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“…Results were tabulated according to concentrations, considering the number of live and dead larvae with 4 replications per treatment, performed in triplicate at 24 and 48 h. Values of 50% lethal concentration (LC 50 ) as well as confidence limits were obtained by probit analysis (Finney 1971) Repeated measures ANOVA and Tukey-Kramer post hoc measurements were performed to compare the means among the different extract treatments under different conditions, using the NCSS statistical software (2019).…”

Section: Discussionmentioning

confidence: 99%

Viability and reconstitution of delta-endotoxins from Bacillus thuringiensis var. israelensis extracts after forty years of storage against Aedes aegypti (Diptera: Culicidae)

Fernández-Chapa

Luna-Olvera

Ramírez-Villalobos

et al. 2021

Egypt J Biol Pest Control

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Background Bacillus thuringiensis subsp. israelensis (Bti) produces insecticidal endotoxins known as Cry and Cyt. Its efficiency and specificity make it the most widely used substance as a biopesticide for controlling disease from vector insects, such as mosquitoes, responsible for important human diseases such as malaria, filariasis, dengue, and yellow fevers. To date, it is proven difficult to develop a commercial product that has more than 2 years of shelf life, and there is little information on the viability of these commercial proteins under prolonged storage conditions. Results This study aimed to evaluate biological activity of reconstituted Bti endotoxins after 40 years of storage against the mosquito Aedes aegypti larvae. Five concentrations of Bti extracts were used for bioassays against 3rd and 4th instars of A. aegypti larvae. All reconstituted endotoxins from stored extracts showed a potency increase. The strain HD-500 from extract 3260 was the most effective insecticide (LC50 = 0.0014 mg/l), followed by 3756 (LC50 = 0.0037 mg/l). These strains were particularly notable, increasing their larvicidal potency one hundredfold and one thousandfold, respectively. Protein profiles in polyacrylamide gels revealed a greater presence of Cyt toxins compared to the stored Bti extracts, which maintained their activity at high concentrations. Conclusion The reconstituted Bti strains presented a great biological activity against A. aegypti larvae, specially extract 3260 (median lethal concentration (LC50) value = 0.0014 mg/l). This considerable larvicidal activity after 40 years under storage was an encouraging signal for the development of future formulation strategies regarding their useful life. The stability of extracts of stored endotoxins produced by Bti decreased significantly, particularly Cyt1A protein, which is responsible for their synergistic activity.

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“…To complement the existing efforts toward malaria control and elimination, in the year 2017, Tanzania scaled up a nationwide biolarviciding program for mosquito control in both urban and rural areas [7,8]. The intervention has the following benefits; assist in fighting against mosquito resistance to insecticides reported in the country; reduce outdoor biting mosquitoes, thus preventing outdoor malaria transmission; and assist in control of other mosquito-borne diseases transmitted by mosquito such as Culex and Aedes species [9][10][11][12]. In Tanzania, the implementation of biolarviciding is done by councils through their health department and in collaboration with community members, and it involves breeding site identification and application of biolarvicide to identified waterbodies and surveillance.…”

Section: Introductionmentioning

confidence: 99%

The Role of Community Participation in Planning and Executing Malaria Interventions: Experience from Implementation of Biolarviciding for Malaria Vector Control in Southern Tanzania

Matindo

Kalolo²,

Kengia

et al. 2022

BioMed Research International

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Background. Malaria remains a disease of great public health importance in 85 countries globally. Developing countries face resource constraints in implementing public health interventions aiming at controlling malaria. Promoting community participation may contribute to rational and effective use of resources and therefore facilitating achievement of intervention goals in a cost-effective manner while fostering sustainability. However, this can be possible if the community is engaged at all stages of the intervention, from designing, implementation, monitoring, and evaluation of results. This study aimed at understanding community participation in the implementation of a biolarviciding intervention for malaria vectors control in Southern Tanzania. Methods. The current study adopted explanatory mixed method study design in collecting data. Quantitative data were collected from 400 community members and 12 vector control coordinators using structured questionnaire while qualitative data was collected through key informant interviews to 32 participants and in-depth interviews to 5 vector control coordinators who were purposively selected from the 12 councils. Quantitative data analysis involved descriptive and inferential statistics. Thematic analysis was used to analyse qualitative data. Results. Of 400 community members, only 90 (22.5%) participated in biolarviciding implementation. Predictors of community participation were willingness to participate ( AOR = 3.15 , 95 % CI = 1.14 − 8.71 , P value = 0.027) and community involvement ( AOR = 6.07 , 95 % CI = 2.69 − 13.71 , P value < 0.001). The study revealed that the main barriers to community participation were lack of effective involvement and lack of incentive to community volunteers while high willingness to participate was a facilitating factor for community participation. Conclusion. The study revealed low community participation in biolarviciding implementation in Southern Tanzania with willingness to participate and community involvement being the main predictors for community participation while lack of incentive to community volunteers was one major barrier to community participation. This explains the persistence of an unresolved challenge of community participation in malaria interventions. Therefore, more efforts are needed to improve the participation of community members in Malaria interventions through advocacy, awareness creation of respective roles, and responsibilities of the community members and fostering community ownership. Additionally, councils need to design customized motivation package for the community members.

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Efficacy of Bacillus thuringiensis israelensis (Bti) and four plant extracts on the mortality and development of Culex quinquefasciatus Say (Diptera: Cullicidae)

Cited by 4 publications

References 9 publications

Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

Biological Control of Aedes aegypti Mosquitoes Using Bacillus thuringiensis

Viability and reconstitution of delta-endotoxins from Bacillus thuringiensis var. israelensis extracts after forty years of storage against Aedes aegypti (Diptera: Culicidae)

The Role of Community Participation in Planning and Executing Malaria Interventions: Experience from Implementation of Biolarviciding for Malaria Vector Control in Southern Tanzania

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