Efficacy of Avena Sativa L. And Trifolium Alexandrinum L. Test System for Detecting the Genotoxic and Phytotoxic Potential of Water Pollution

Abstract

Abstract. The Reghaïa Lake (Algiers, Algeria) is contaminated by the untreated industrial effluents discharge of Reghaïa-Rouiba industrial park, via El Biar River. The present report is the first study which addresses the effects of industrial wastewaters rejected into the lake in light of genotoxic and phytotoxic effects, using Avena sativa L. and Trifolium alexandrinum L. test system. The wastewaters were collected seasonally at the mouth of El Biar River and analyzed using chemical analysis and biological assays, including the germination and root elongation inhibition test, mitotic index and micronucleus test. Results showed that samples collected during the summer season (SM) were high contaminated by heavy metals than that of the winter season (WN). Furthermore, the WN samples have relatively low phytotoxicity on A. sativa seed germination and T. alexandrinum root elongation (no EC50 was determined). Genotoxicity of the SM samples is evident in A. sativa by a significant depression of mitotic activity, induction of several chromosomal aberrations and occurrence of micronuclei in T. alexandrinum. All the results indicated that the samples from the El Biar discharging river in Reghaïa Lake exhibited genotoxicity and argue for the possible use of A. sativa and T. alexandrinum plant system for genotoxicity assays.

Evaluation of Heavy Metal Presence in Agricultural Samples of Lactuca sativa and Trifolium alexandrinum Using Picosecond Laser-Induced Breakdown Spectroscopy and Flame Atomic Absorption Spectroscopy in Banha and Giza Governorates, Egypt

“…Trifolium alexandrinum, commonly known as Egyptian clover, is a key forage crop, and Lactuca sativa, or lettuce, is widely consumed as a leafy vegetable. Both are integral to human and animal diets and often serve as bioindicators for environmental health [13]. Their widespread cultivation and consumption make them pivotal in studies focusing on trace metal accumulation and its subsequent impact on human and animal health [14].…”