Efficacy of Ascorbic Acid in Reducing Glyphosate-Induced Toxicity in Rats

Abstract

Aim: Humans and animals interact with their environments on a daily basis and, as a consequence, are exposed to a broad spectrum of synthesized chemicals present in the food they eat, the air they breathe and the water they drink including glyphosate. This study was aimed at investigating the effects of glyphosate on the sperm dynamics of male albino rats and the protective effects of ascorbic acid. Methods: Twenty five mature male albino rats were weighed and divided into five groups in a completely randomized design (CRD). Group 1 rats served as the control. Rats in groups 2 and 4 received 250ml/kg and 500ml/kg of glyphosate while groups 3 and 5 rats were administered with 250ml/kg and 500ml/kg of glyphosate and 200mg/kg of ascorbic acid, respectively, which were administered orally using oral gavages. The treatment regimen lasted for 65 days. Results: Our results showed that there were significant adverse effects (P<0.05) of glyphosate treatment on sperm parameters and the cyto-architecture of the gonad, which showed disruption in the seminiferous tubules, necrotic germinal epithelium and clumped Leydig cells. However, administering the rats with ascorbic acid caused significant ameliorating effects on the parameters investigated. Conclusion: Succinctly, glyphosate exposure to animals is detrimental to their reproductive physiology, including the cellular integrity of the gonads. This not

Attenuating Potential of Trévo Dietary Supplement on Caffeine Induced Sperm Toxicities in Albino Rats

“…Results obtained in the study revealed that caffeine caused a significant reduction in the weight of testes and epididymis, sperm count, viability and motility which agrees with the findings of Wilcox et al (1988), Ekaluo et al (2009) and Ikpeme et al (2012) and also significantly increased the percentage of sperm head abnormalities which is similar to the findings of Ekaluo et al (2009Ekaluo et al ( , 2013a and Ikpeme et al (2012).…”

Protective Role of Aqueous Guava Leaf Extract Against Caffeine Induced Spermatotoxicity in Albino Rats

“…The significant reduction in the sperm count and viability, in caffeine treated animals is similar to the findings of Wilcox et al (1988), Bassey et al (2011) and Ekaluo et al (2013c, d) and also significantly increased the percentage of sperm head abnormalities which is similar to the findings of Ikpeme et al (2012).…”

Reproductive toxicity of soybean (Glycine max L.) in rat

“…Soybean meal did not have any significant effect on weight of testes and epididymes which was not contrary to the earlier reports of Ikpeme et al [15];Ekaluo et al [18]. However, it significantly reduced sperm viability and sperm count, while increasing sperm head abnormality in a dose-dependent manner; and their relationships agreed with the reports of Ekaluo et al [10,12,16,18] on sperm parameters which show reproductive toxicity on the reproductive endpoints of sperm count, sperm viability and sperm head abnormality.…”

“…The testes were fixed in Bouin's fluid for 24 hours then processed and stained using the hematoxylin and eosin (H&E) differential staining technique [15].…”

“…The suspension was filtered using an 80μm stainless mesh. Caudal epididymal sperm count was obtained by cytometry using the improved Neubauer cytometer and was expressed as million/ml of the sperm suspension [15,16].…”