Efficacy of an inactivated, recombinant bovine herpesvirus type 5 (BoHV-5) vaccine

Campos, Fabrício Souza; Dezen, Diogenes; Antunes, Dinler Amaral; Santos, Helton Fernandes dos; Arantes, Thalita Souza; Cenci, Alexander; Gomes, Fabrício Maciel; Lima, Francisco Esmaile Sales; Brito, Wilia Marta Elsner Diederichsen de; Filho, Hiran Castagnino Kunert; Batista, Helena Beatriz de Carvalho Ruthner; Spilki, Fernando Rosado; Franco, Ana Cláudia; Rijsewijk, F.A.M.; Roehe, Paulo Michel

doi:10.1016/j.vetmic.2010.08.004

Cited by 10 publications

(8 citation statements)

References 41 publications

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“…Bovine herpesvirus-5 (BoHV-5) is an alphaherpesvirus associated with bovine meningoencephalitis that has been reported in South America, Australia and in other continents (Perez et al, 2003). Although outbreaks of meningoencephalitis can reach a mortality rate of 70-100% (Riet-Correa et al, 2006), causing important economic losses (Campos et al, 2009), BoHV-5 vaccines remain at an experimental stage of development (Campos et al, 2011).…”

Section: Introduction mentioning

confidence: 99%

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

Roos

Ávila

Sturbelle

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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There have been significant efforts towards the development of more efficient vaccines for animal health. A strategy that may be used to improve vaccine efficacy is the use of probiotics to enhance the immune response of the host, leading to increased immunogenicity of antigen preparations. Bovine herpesvirus 5 (BoHV-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. In this study, we examined the use of the probiotic Saccharomyces boulardii (Sb) as a potential adjuvant to improve vaccine efficiency. We found that the supplemented animals exhibited an enhanced systemic IgG antibody response toward a Th1 response in favor of IgG2a and increased mRNA expression levels of the cytokines IFN-y, IL-12, IL-17 and IL-10 in the spleen. These results suggest that Sb supplementation may provide a promising means for improving the efficiency of vaccines, particularly those that rely on a cell-mediated immune response.

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Section: Introduction mentioning

confidence: 99%

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

Roos

Ávila

Sturbelle

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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“…In Brazil, BoHV-1 and BoHV-5 gE-deleted marker vaccines have been developed and tested but are not yet commercially available [22,23,26e31]. Marker vaccines (live or inactivated) represent a good choice for disease control as they protect cattle clinically in case of infection and markedly reduce the subsequent shedding of wild-type virus [22,23,32]. A recombinant BoHV-5 lacking thymidine kinase and gE genes was evaluated as a live experimental vaccine and conferred protection upon homologous and heterologous (BoHV-1) challenge [22].…”

Section: Introductionmentioning

confidence: 99%

“…A recombinant BoHV-5 lacking thymidine kinase and gE genes was evaluated as a live experimental vaccine and conferred protection upon homologous and heterologous (BoHV-1) challenge [22]. Campos and co-workers showed that a BoHV-5 gI/gE/US9 e inactivated marker vaccine was capable of conferring protection against encephalitis when vaccinated cattle were challenged with a large infectious dose of the parental wildtype BoHV-5 [23]. Furthermore, Romera and colleagues developed and evaluated a gE-deleted BoHV-1 marker vaccine strain as an inactivated and a live attenuated vaccine preparation.…”

Section: Introductionmentioning

confidence: 99%

Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

Siedler

Roloff

Sá

et al. 2017

Protein Expression and Purification

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Bovine herpesvirus (BoHV) glycoprotein E (gE) is a non-essential envelope glycoprotein and the deletion of gE has been used to develop BoHV-1 and BoHV-5 differential vaccine strains. The DIVA (Differentiation of Infected from Vaccinated Animals) strategy, using marker vaccines based on gE-negative BoHV strains, allows the identification of vaccinated or infected animals in immunoassays designed to detect anti-gE antibodies. In this study a codon optimized synthetic sequence of gE containing highly conserved regions from BoHV-1 and BoHV-5 was expressed in Pichia pastoris. Following expression, the recombinant gE (rgE) was secreted and purified from the culture medium. The rgE was identified by Western blotting (WB) using sera from cattle naturally infected with BoHV-1 and/or BoHV-5, or sera from bovines experimentally infected with wild-type BoHV-5. Sera collected from cattle vaccinated with a BoHV-5 gI/gE/US9¯ marker vaccine failed to recognise rgE. Expression of rgE, based on a sequence containing highly conserved regions from BoHV-1 and BoHV-5, in P. pastoris enabled the production of large quantities of rgE suitable for use in immunoassays for the differentiation vaccinated or infected cattle.

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“…Thus, the use of BoHV-1 gE − live marker vaccine where BoHV-5 prevalence is higher may be unsuitable. In 2007, Franco et al [ 17 ] reported the construction of a BoHV-5 gI/gE/US9 − and the resulting virus has been tested as an inactivated vaccine in 2010 [ 18 ], conferring protection to encephalitis after challenge with a high dose of virulent BoHV-5 and reduction of nasal virus shedding in vaccinated animals.…”

Section: Introductionmentioning

confidence: 99%

Bovine herpesvirus glycoprotein D: a review of its structural characteristics and applications in vaccinology

Dummer

Hurk

2014

Vet Res

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The viral envelope glycoprotein D from bovine herpesviruses 1 and 5 (BoHV-1 and -5), two important pathogens of cattle, is a major component of the virion and plays a critical role in the pathogenesis of herpesviruses. Glycoprotein D is essential for virus penetration into permissive cells and thus is a major target for virus neutralizing antibodies during infection. In view of its role in the induction of protective immunity, gD has been tested in new vaccine development strategies against both viruses. Subunit, DNA and vectored vaccine candidates have been developed using this glycoprotein as the primary antigen, demonstrating that gD has the capacity to induce robust virus neutralizing antibodies and strong cell-mediated immune responses, as well as protection from clinical symptoms, in target species. This review highlights the structural and functional characteristics of BoHV-1, BoHV-5 and where appropriate, Human herpesvirus gD, as well as its role in viral entry and interactions with host cell receptors. Furthermore, the interactions of gD with the host immune system are discussed. Finally, the application of this glycoprotein in new vaccine design is reviewed, taking its structural and functional characteristics into consideration.

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Efficacy of an inactivated, recombinant bovine herpesvirus type 5 (BoHV-5) vaccine

Cited by 10 publications

References 41 publications

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

Bovine herpesvirus glycoprotein D: a review of its structural characteristics and applications in vaccinology

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