Efficacy of Acacia nilotica aqueous extract in treating biofilm-forming and multidrug resistant uropathogens isolated from patients with UTI syndrome

Abstract

Escherichia coli is the dominant bacterial cause of UTI among the uropathogens in both developed and developing countries. This study is to investigate the effect of Acacia nilotica aqueous extract on the survival and biofilm of isolated pathogens to reduce UTIs diseases. A total of 170 urine samples were collected from Luxor general hospital and private medical analysis laboratories in Luxor providence, Egypt. Samples were screened for the incidence of uropathogens by biochemical tests, antibiotics susceptibility, detection of virulence, and antibiotic-resistant genes by multiplex PCR, biofilm formation, and time-killing assay. Escherichia coli is by far the most prevalent causative agent with the percentage of 73.7% followed by Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeuroginosa, and Acinetobacter baumanii. Isolates were multidrug-resistant containing blaTEM, blaSHV, blaCTX, qnrs, and aac(3)-Ia resistant genes. All isolates were sensitive to 15–16.7 mg ml−1 of Acacia nilotica aqueous extract. Time killing assay confirmed the bactericidal effect of the extract over time (20–24 h). A high percentage of 3-Cyclohexane-1-Carboxaldehyde, 2,6,6-trimethyl (23.5%); á-Selinene (15.12%); Oleic Acid (14.52%); Globulol (11.35%) were detected among 19 bioactive phytochemical compounds in the aqueous extract of A. nilotica over the GC-mass spectra analysis. The plant extract reduced significantly the biofilm activity of E. coli, K. pneumoniae, P. mirabilis, and P. aeuroginosa by 62.6, 59. 03, 48.9 and 39.2%, respectively. The challenge to improve the production of A. nilotica phytochemicals is considered a very low price for the return.

Efficacy of Ethanolic Extract of Syzygium aromaticum in the Treatment of Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates Associated with Urinary Tract Infections

“…Each organism has its encoding virulence gene(s) that is responsible for the pathogenicity of an infective agent [5]. P. aeruginosa is well known as an opportunistic bacterial pathogen [6], essentially dangerous to patients suffering from cystic fibrosis and people with a weak immune system [7].…”

Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria

“…The principal method for preventing biofilm formation is applying chemicals or antimicrobials, such as chemical biocides, detergents, and surfactants. Biofilm destruction and prevention are effective methods, as are mechanical removal techniques such as shredding, sonication, freezing, and thawing ( De Carvalho, 2007 ; Kalpana, Aarthy & Pandian, 2012 ; Elamary, Albarakaty & Salem, 2020 ). However, because the exopolysaccharide biofilm cells are protected ( Kalpana, Aarthy & Pandian, 2012 ), it is difficult to completely remove biofilms using these methods.…”

Efficacy of Soil ParaBurkholderia fungorum and Bacillus subtilis on the Inhibition of Aspergillus niger Growth and its Ochratoxins Production

“…This analysis confirmed the presence of high contents of palmitic acid; 9,12-Octadecadienoic acid (Z,Z)-, trimethylsilyl ester, and Oleic Acid, (Z)-(Table 4). These compounds were confirmed previously to be excellent antifungal agents (McGaw et al, 2002;Seidel & Taylor, 2004;Agoramoorthy et al, 2007;Jung et al, 2013;Elamary et al, 2020). Recently, it was verified that volatile organic compounds (VOCs), such as hydrocarbons, alcohols, ketones, aldehydes, ethers, esters, terpenes, terpene derivatives, and several heteroaromatic compounds that were formed by certain bacteria exhibit antifungal activity (Alstrom, 2001;Wheatley, 2002;Schalchli et al, 2011).…”