Efficacy of a single intravenous dose of the neuraminidase inhibitor peramivir in the treatment of equine influenza

Yamanaka, Takashi; Bannai, Hiroshi; Nemoto, Manabu; Tsujimura, Koji; Kondo, Takashi; Muranaka, Masanori; Hobo, Seiji; Minamijima, Yohei; Yamada, Masayuki; Matsumura, Tomio

doi:10.1016/j.tvjl.2012.01.005

Cited by 18 publications

(18 citation statements)

References 30 publications

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“…NA has been the target of second-generation antiviral drugs to inhibit its function and prevent virus propagation. Although not routinely used in horses, NA inhibitors have been shown to have potential for the treatment of EIV infection [5,6]. …”

Section: Introductionmentioning

confidence: 99%

Evolution and Divergence of H3N8 Equine Influenza Viruses Circulating in the United Kingdom from 2013 to 2015

et al. 2017

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Equine influenza viruses (EIV) are a major cause of acute respiratory disease in horses worldwide and occasionally also affect vaccinated animals. Like other influenza A viruses, they undergo antigenic drift, highlighting the importance of both surveillance and virus characterisation in order for vaccine strains to be kept up to date. The aim of the work reported here was to monitor the genetic and antigenic changes occurring in EIV circulating in the UK from 2013 to 2015 and to identify any evidence of vaccine breakdown in the field. Virus isolation, reverse transcription polymerase chain reaction (RT-PCR) and sequencing were performed on EIV-positive nasopharyngeal swab samples submitted to the Diagnostic Laboratory Services at the Animal Health Trust (AHT). Phylogenetic analyses were completed for the haemagglutinin-1 (HA1) and neuraminidase (NA) genes using PhyML and amino acid sequences compared against the current World Organisation for Animal Health (OIE)-recommended Florida clade 2 vaccine strain. Substitutions between the new isolates and the vaccine strain were mapped onto the three-dimensional structure protein structures using PyMol. Antigenic analyses were carried out by haemagglutination inhibition assay using a panel of post-infection ferret antisera. Sixty-nine outbreaks of equine influenza in the UK were reported by the AHT between January 2013 and December 2015. Forty-seven viruses were successfully isolated in eggs from 41 of the outbreaks. Only three cases of vaccine breakdown were identified and in each case the vaccine used contained a virus antigen not currently recommended for equine influenza vaccines. Nucleotide sequencing of the HA and NA genes revealed that all of the viruses belonged to the Florida clade 2 sub-lineage of H3N8 EIV. Phylogenetic and sequence analyses showed that the two sub-populations, previously identified within clade 2, continued to circulate and had accrued further amino acid substitutions. Antigenic characterisation using post-infection ferret antisera in haemagglutination inhibition assays however, failed to detect any marked antigenic differences between the isolates. These findings show that Florida clade 2 EIV continue to circulate in the UK and support the current OIE recommendation to include an example of Florida clade 2 in vaccines.

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Section: Introductionmentioning

confidence: 99%

Evolution and Divergence of H3N8 Equine Influenza Viruses Circulating in the United Kingdom from 2013 to 2015

et al. 2017

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“…To the best of our knowledge, only three detailed analytical methods and one brief quantification description have been reported in English language journals, by Lindegardh et al [5], Li et al [9,10] and Yamanaka et al [11]. Some of these used a HILIC for the extraction and analytical procedure [5,9].…”

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confidence: 99%

“…Another method measuring horse plasma of peramivir by directed SPE combined with LC separation has been carried out by Yamanaka et al [11]. A relatively high concentration of acetonitrile was needed to promote interactions between the compounds and acquire enough recovery, and then trapped the lipophilic phospholipids in HILIC SPE columns by selecting elution solution to methanol-formic acid 0.1% (20:80, v/v) [5].…”

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confidence: 99%

“…These published methods for the analysis of peramivir are complicated by either phospholipid adhesion or a need for derivatization to achieve the sensitivity required by regulatory authorities. Moreover, most of the LC-MS/MS methods already described that use derivatization before analysis were developed using plasma from animals [10][11][12] and there is little information about the chromatographic behavior of peramivir in human plasma.…”

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A Rapid LC–MS/MS Quantification of Peramivir Using A Simple and Inexpensive Sample Precipitation: Application to Pk

et al. 2015

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“…The manuscript by Dr. Takahashi Yamanaka from the Equine Research Institute of the Japan Racing Association, and his colleagues, published in this issue of The Veterinary Journal describes a pilot study of the influenza antiviral agent peramivir in horses (Yamanaka et al, 2012). Intravenous administration of a single dose of peramivir as soon as pyrexia or shedding of virus was detected in experimentally-infected horses reduced the duration of virus shedding and the severity of clinical signs compared to placebo-treated controls.…”

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confidence: 99%

Neuraminidase inhibitors for treatment of equine influenza – when all else fails?

Daly

2012

The Veterinary Journal

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Efficacy of a single intravenous dose of the neuraminidase inhibitor peramivir in the treatment of equine influenza

Cited by 18 publications

References 30 publications

Evolution and Divergence of H3N8 Equine Influenza Viruses Circulating in the United Kingdom from 2013 to 2015

Evolution and Divergence of H3N8 Equine Influenza Viruses Circulating in the United Kingdom from 2013 to 2015

A Rapid LC–MS/MS Quantification of Peramivir Using A Simple and Inexpensive Sample Precipitation: Application to Pk

Neuraminidase inhibitors for treatment of equine influenza – when all else fails?

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