Efficacy of a selective isolation procedure for members of the Pseudallescheria boydii complex

Rainer, Johannes Michael; Kaltseis, Josef; Hoog, Sybren G. de; Summerbell, Richard C.

doi:10.1007/s10482-007-9206-y

Cited by 63 publications

(63 citation statements)

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“…on routine media (4). A medium (SceSelϩ) containing the selective antifungal agents dichloran and benomyl has been described for the recovery of Scedosporium from environmental samples (16,20), but its utility in detecting Scedosporium spp. in clinical specimens has not been determined.…”

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“…Sputa were inoculated onto (i) Sabouraud dextrose agar (SABD; Difco, Becton Dickinson, Franklin Lakes, NJ) containing chloramphenicol (250 mg/liter) and gentamicin (26 mg/ liter), (ii) Mycosel (BBL, Becton Dickinson), and (iii) SceSelϩ medium (20). Plates were incubated at 30°C in air and examined twice weekly for 28 days.…”

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confidence: 99%

“…In conclusion, we propose that a Scedosporium-selective medium such as SceSelϩ (20) be used in combination with routine media when examining patients with chronic lung diseases, including CF, for filamentous fungi. Sensitive, culture-based methods should be used before lung transplantation to identify all potential pathogens, and species identity should be confirmed by molecular methods.…”

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Detection of Occult Scedosporium Species in Respiratory Tract Specimens from Patients with Cystic Fibrosis by Use of Selective Media

et al. 2010

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Respiratory samples from cystic fibrosis outpatients were cultured on Sabouraud's dextrose agar (SABD) containing antibiotics, Mycosel, and Scedosporium-selective medium (SceSel؉). Thirty-two (14.7%) of 218 specimens from 11/69 (15.9%) patients yielded a Scedosporium sp., most frequently Scedosporium aurantiacum (17/218). Scedosporium was recovered on SceSel؉, Mycosel, and SABD from 90.6%, 50.0%, and 46.9% of the specimens tested, respectively.Opportunistic molds are increasingly isolated in respiratory specimens from children and adults with cystic fibrosis (CF) (17). Scedosporium species are the second most frequently recovered fungi, after Aspergillus fumigatus (4). Although invasive scedosporiosis is reportedly rare in CF prior to lung transplantation (11), colonization may be a risk factor for invasive disease posttransplantation, with associated high mortality (14,19,(21)(22)(23). The impact of airway colonization by Scedosporium spp. on respiratory function has not been studied; nevertheless, as Scedosporium spp. are resistant to many antifungal agents, colonization may be a relative contraindication to transplantation (18).Isolation of multiple fungi from respiratory specimens is frequent (19). The prevalence of non-Aspergillus molds may be underestimated due to overgrowth of Aspergillus spp. on routine media (4). A medium (SceSelϩ) containing the selective antifungal agents dichloran and benomyl has been described for the recovery of Scedosporium from environmental samples (16,20), but its utility in detecting Scedosporium spp. in clinical specimens has not been determined. No commercial preparation is available. Scedosporium spp. have been identified previously in respiratory specimens from 8.6 to 10% of CF patients (4, 24). These data were published prior to recent taxonomic reassignments of Scedosporium spp. (9, 10), and selective media were not used. We therefore compared the performance of two general media and one selective medium for the isolation of Scedosporium spp. from respiratory specimens and prospectively investigated the frequency and species distribution of Scedosporium in CF patients. Plates were incubated at 30°C in air and examined twice weekly for 28 days. Suspicious colonies were identified to the species level (Aspergillus and Scedosporium spp.) or the genus level (other molds) by standard morphological/ phenotypic methods (7, 9, 10). Scedosporium strains were identified as Scedosporium prolificans, S. aurantiacum, or S. apiospermum by restriction fragment length analysis of the internal transcribed spacer (ITS) region (8). ITS sequence analysis was performed on all S. apiospermum isolates and selected S. aurantiacum isolates (8).Samples (n ϭ 218) were received from 69 patients. Filamentous fungi were detected in 142 samples (65.1%) from 56 patients (81.2%; Table 1). The majority of the fungi (83.9%) were recovered following 6 days of incubation, and 95.1% were recovered following 10 days. Aspergillus spp. were the most frequently recovered, with A. fumigatus isolated in 45.4% of t...

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Detection of Occult Scedosporium Species in Respiratory Tract Specimens from Patients with Cystic Fibrosis by Use of Selective Media

et al. 2010

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“…kolonizasyona predispozisyon oluşturması ve etken virülansına özel hasta gruplarının tanımlanması (tatlı suda boğulma olaylarında beyin apsesi oranlarının artması vb.) ile hayatı tehdit eden, önem kazanan fungal patojenler arasında kabul edilmeye başlanmıştır 4,[7][8][9][10] . Risk grubu hastalarda çoğunlukla ön planda Aspergillus ve Candida izolasyonlarının olması, daha yavaş üreyen Scedosporium spp.…”

Section: Discussionunclassified

Isolation of Scedosporium apiospermum (Teleomorph: Pseudallescheria apiosperma) from an Acute Myeloid Leukemia Patient

Ergin

Kutlu²,

Akdağlı³

et al. 2013

Mikrobiyol Bul

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* Bu çalışma, I. Ulusal Klinik Mikrobiyoloji Kongresi (12-16 Kasım 2011, Antalya)'nde sunulmuştur. ÖZETScedosporium apiospermum özellikle immün sistemi baskılanmış kişilerde hayatı tehdit eden enfeksiyonlar oluşturabilen fırsatçı bir patojendir. Bu raporda, 62 yaşındaki erkek, akut miyeloid lösemi hastasın-da gelişen S.apiospermum enfeksiyonu sunulmaktadır. Remisyon-indüksiyon kemoterapisi sırasında nöt-ropenik ateş gelişen hastaya piperasilin-tazobaktam tedavisi başlanmıştır. Ateş yüksekliğinin devam etmesi nedeniyle imipenem tedavisine geçilmiş ve ampirik amfoterisin B deoksikolat tedaviye eklenmiştir. Amfoterisin B alerjisi nedeniyle, ateşin beşinci gününde kaspofungin tedavisi başlanmıştır. Yüksek çözünür-lüklü toraks tomografisi değerlendirmesi sonucunda, invazif aspergilloz düşüncesiyle antifungal tedavi vorikonazol olarak değiştirilmiştir. Vorikonazol tedavisinin başladığı gün galaktomannan testinin negatif bulunması üzerine, akciğer sol alt lob apikal segmentten fiberoptik bronkoskop ile alınan örnekler çeşitli Geliş Tarihi

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“…Swabs were plated out on Sabouraud agar and on Scel+ agar to select for Scedosporium sp (9). All plates were incubated at 27 o C for up to two weeks.…”

Section: Laboratory Investigationsmentioning

confidence: 99%

Fungal contamination of nebuliser devices used by people with cystic fibrosis

Peckham

Williams

Wynne

et al. 2016

Journal of Cystic Fibrosis

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Efficacy of a selective isolation procedure for members of the Pseudallescheria boydii complex

Cited by 63 publications

References 8 publications

Detection of Occult Scedosporium Species in Respiratory Tract Specimens from Patients with Cystic Fibrosis by Use of Selective Media

Detection of Occult Scedosporium Species in Respiratory Tract Specimens from Patients with Cystic Fibrosis by Use of Selective Media

Isolation of Scedosporium apiospermum (Teleomorph: Pseudallescheria apiosperma) from an Acute Myeloid Leukemia Patient

Fungal contamination of nebuliser devices used by people with cystic fibrosis

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