Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants

Aguirreburualde, María Sol Pérez; Gómez, María Cristina Maldonado; Ostachuk, Agustin; Wolman, Federico Javier; Albanesi, Guillermo; Pécora, Andrea; Odeón, Anselmo; Ardila, Fernando; Escribano, José M.; Santos, María José Dus; Wigdorovitz, Andrés

doi:10.1016/j.vetimm.2012.12.004

Cited by 53 publications

(32 citation statements)

References 49 publications

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“…However, the IgG1/IgG2 ratio showed a difference which suggests a special immune response pattern. The IgG1/IgG2 ratio obtained was similar to that of other reports using APCH fused to the E2t protein as a subunit vaccine for Bovine viral diarrhea virus [37]. These results could indicate that the APCH molecule slightly switches the isotype profile toward IgG1.…”

Section: Discussionsupporting

confidence: 88%

“…Specifically, similar titers were reached for treatments including BEI-inactivated vaccine, VP2-and the APCH-VP2-based vaccines, although a four-fold lower antigenic mass was used in the APCH-VP2 group. The APCH molecule has been described as an immune response enhancer when it was fused to rabbit hemorrhagic disease virus, Canine Parvovirus, and Bovine viral diarrhea subunit vaccines [20,37,38]. In those reports, different expression systems as baculoviruses (sf9 cells and Tricoplusia nii larvae) or molecular farming (Medicago sativa L.) were used to express the recombinant antigens fused to APCH.…”

Section: Discussionmentioning

confidence: 99%

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An experimental subunit vaccine based on Bluetongue virus 4 VP2 protein fused to an antigen-presenting cells single chain antibody elicits cellular and humoral immune responses in cattle, guinea pigs and IFNAR(−/−) mice

Legisa¹,

Aguirreburualde²,

Gonzalez³

et al. 2015

Vaccine

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

An experimental subunit vaccine based on Bluetongue virus 4 VP2 protein fused to an antigen-presenting cells single chain antibody elicits cellular and humoral immune responses in cattle, guinea pigs and IFNAR(−/−) mice

Legisa¹,

Aguirreburualde²,

Gonzalez³

et al. 2015

Vaccine

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“…6 Thus, the use of immunodominant BVDV protein (E2) as a subunit vaccine may be a useful tool for the development of efficient strategies to control the virus. 10,14 The baculovirus-insect cell expression system (Bevs, the abbreviature of Baculovirus expression vector system) is one of the most widely used tools for the high level expression of heterologous proteins in a eukaryotic host and was chosen to express tE2 and APCH-tE2 immunogens in this work. Advantages include the production of high level foreign proteins as well as the insect cells capable of posttranslational modifications similar to those of mammalian cells.…”

Section: Desarrollo De Una Vacuna De Subunidad Bvdv Mejorada Basada Ementioning

confidence: 99%

Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells

Pécora¹,

Malacari²,

Aguirreburualde³

et al. 2015

Revista Argentina de Microbiología

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Bovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines. To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed in the baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected for a cattle vaccination experiment. All the bovines immunized with 1.5 μg of APCH-tE2 developed high levels of neutralizing antibodies against BVDV up to a year post-immunization, demonstrating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-up and was transferred to the private sector. Nowadays, it is being evaluated for registration as the first Argentinean subunit vaccine for cattle.

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“…BVDV E2 protein has previously been expressed in mammalian expression system, [9,10] baculovirus expression system, [9,11] Drosophila A c c e p t e d M a n u s c r i p t 4 melanogaster system, [12] Saccharomyces cerevisiae yeast system [13] and alfalfa plants [14] . In this study, BVDV E2 protein was expressed in Pichia pastoris yeast for the first time and its potential use as a candidate antigen was evaluated in ELISA for detection of BVDV neutralizing antibodies in cattle.…”

Section: Expression Of Bovine Viral Diarrhoea Virus Envelope Glycopromentioning

confidence: 99%

Expression of Bovine Viral Diarrhea Virus Envelope Glycoprotein E2 in YeastPichia pastorisand its Application to an ELISA for Detection of BVDV Neutralizing Antibodies in Cattle

Behera

Mishra

Nema

et al. 2015

Journal of Immunoassay and Immunochemistry

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The aim of this article is to express envelope glycoprotein E2 of bovine viral diarrhea virus (BVDV) in yeast Pichia pastoris and its utility as a diagnostic antigen in ELISA. The BVDV E2 gene was cloned into the pPICZαA vector followed by integration into the Pichia pastoris strain X-33 genome for methanol-induced expression. SDS-PAGE and Western blot results showed that the recombinant BVDV E2 protein (72 kDa) was expressed and secreted into the medium at a concentration of 40 mg/L of culture under optimized conditions. An indirect ELISA was then developed by using the yeast-expressed E2 protein. Preliminary testing of 300 field cattle serum samples showed that the E2 ELISA showed a sensitivity of 91.07% and a specificity of 92.02% compared to the reference virus neutralization test. The concordance between the E2 ELISA and VNT was 91.67%. This study demonstrates feasibility of BVDV E2 protein expression in yeast Pichia pastoris for the first time and its efficacy as an antigen in ELISA for detecting BVDV neutralizing antibodies in cattle.

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Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants

Cited by 53 publications

References 49 publications

An experimental subunit vaccine based on Bluetongue virus 4 VP2 protein fused to an antigen-presenting cells single chain antibody elicits cellular and humoral immune responses in cattle, guinea pigs and IFNAR(−/−) mice

An experimental subunit vaccine based on Bluetongue virus 4 VP2 protein fused to an antigen-presenting cells single chain antibody elicits cellular and humoral immune responses in cattle, guinea pigs and IFNAR(−/−) mice

Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells

Expression of Bovine Viral Diarrhea Virus Envelope Glycoprotein E2 in YeastPichia pastorisand its Application to an ELISA for Detection of BVDV Neutralizing Antibodies in Cattle

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