Efficacy of a baculovirus pesticide expressing an eclosion hormone gene

Eldridge, Russ; O’Reilly, David R.; Miller, Lo is K.

doi:10.1016/1049-9644(92)90033-a

Cited by 29 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed no difference in infectivity between AcMNPV WT and AcMNPV Degt in 3rd instar larvae of S. exigua and T. ni. This is congruent with most other studies, where no effect of absence of the egt gene on viral infectivity was found, for AcMNPV in S. frugiperda (O'Reilly & Miller 1991;Eldridge et al 1992b), in T. ni (Eldridge et al 1992a) and in S. exigua (Bianchi et al 2000), for SfMNPV in S. frugiperda (Sim on et al 2012), for LdMNPV in L. dispar (Slavicek et al 1999) and for Hear-NPV in Helicoverpa armigera (Georgievska et al 2010). Only one study found that the infectivity of AcMNPV Δegt was higher than that of AcMNPV WT, in 5th instars of T. ni (Cory et al 2004).…”

Section: Discussionsupporting

confidence: 92%

Baculovirus‐induced tree‐top disease: how extended is the role ofegtas a gene for the extended phenotype?

et al. 2014

View full text Add to dashboard Cite

Many parasites alter host behaviour to enhance their chance of transmission. Recently, the ecdysteroid UDP-glucosyl transferase (egt) gene from the baculovirus Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) was identified to induce tree-top disease in L. dispar larvae. Infected gypsy moth larvae died at elevated positions (hence the term tree-top disease), which is thought to promote dissemination of the virus to lower foliage. It is, however, unknown whether egt has a conserved role among baculoviruses in inducing tree-top disease. Here, we studied tree-top disease induced by the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in two different host insects, Trichoplusia ni and Spodoptera exigua, and we investigated the role of the viral egt gene therein. AcMNPV induced tree-top disease in both T. ni and S. exigua larvae, although in S. exigua a moulting-dependent effect was seen. Those S. exigua larvae undergoing a larval moult during the infection process died at elevated positions, while larvae that did not moult after infection died at low positions. For both T. ni and S. exigua, infection with a mutant AcMNPV lacking egt did not change the position where the larvae died. We conclude that egt has no highly conserved role in inducing tree-top disease in lepidopteran larvae. The conclusion that egt is a 'gene for an extended phenotype' is therefore not generally applicable for all baculovirus-host interactions. We hypothesize that in some baculovirus-host systems (including LdMNPV in L. dispar), an effect of egt on tree-top disease can be observed through indirect effects of egt on moulting-related climbing behaviour.

show abstract

Section: Discussionsupporting

confidence: 92%

Baculovirus‐induced tree‐top disease: how extended is the role ofegtas a gene for the extended phenotype?

et al. 2014

View full text Add to dashboard Cite

show abstract

“…However, determination of identity with the natural hormone awaits further analysis by mass spectrometry and other physico-chemical methods. Eldridge et al (136) recently examined the efficacy of this EH-expressing baculovirus as a pesticide by infecting the fall armyworm, Spodoptera frugiperda, where Mas-EH was confirmed to be biologically active. Expression of Mas-EH in fifth or sixth (last) instars had no effect on the ability of AcMNPV to limit insect feeding or hasten death.…”

Section: Eclosion Hormonesmentioning

confidence: 97%

Insect Neuropeptides

Kelly

Masler

Menn

1993

Natural and Engineered Pest Management Agents

View full text Add to dashboard Cite

The physiological processes regulated by insect neuropeptides are extensive and include growth, development, molting, reproduction, diapause, behavior, color change, ion and water balance, and muscle contraction. Nearly 80 novel insect neuropeptides have been sequenced to date, 10 or so gene sequences have been determined, numerous analogs have been synthesized, and neuropeptide genes have been expressed in vector systems. Investigations into sites of synthesis and release, and tissue specificity and action, continue to reveal complexity in the (classically simple) insect neuroendocrine system. The rapidly increasing knowledge in this area suggests that useful prototypes for the design of selective pest control agents will emerge in the forseeable future.The field of insect neuroendocrinology has expanded rapidly, fostering numerous reviews within the past five years (1-21), including one book entirely devoted to insect neurohormones (22) and one to insect neuropeptides (23). These publications discuss the discovery, physiology, history and chemistry of insect neuropeptides and present potential applications to pest control. These applications may include: blocking neuropeptide synthesis through expression of antisense RNA or inhibition of specific processing enzymes; inactivation of circulating neuropeptides by binding with specific antibodies, antipeptides or by degradation with specific peptidases; disruption of target binding or signal transduction by peptidomimetics; and disruptive expression of natural or modified neuropeptides through baculoviruses or other expression vectors. As suspected in 1988 (4), the number of purified and sequenced insect neuropeptides has continued to increase, and many of the corresponding nucleotide sequences have followed in rapid succession. Nearly eighty novel amino acid sequences for insect neuropeptides have been identified, and novel nucleotide sequences approach ten. This review updates the recent progress in identification of insect neuropeptide structures and their genes and other areas relevant to the development of pest control agents, This chapter not subject to U.S.

show abstract

“…Samples were stored at -80°C until further use or used directly for RNA extraction. Sf9 cells were maintained at 27 o C in TC-100 medium (Gibco BRL, USA), supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco BRL, USA) as described by standard methods (O'Reilly et al, 1992).…”

Section: Methodsmentioning

confidence: 99%

Molecular Cloning, Gene Structure, Expression, and Enzyme Activity of a Serine Protease from Water Scorpion, Laccotrephes japonensis (Hemiptera: Nepidae)

Park¹,

Choi²,

Nam³

et al. 2012

International Journal of Industrial Entomology

View full text Add to dashboard Cite

Serine proteases are major insect enzymes involved in the digestion of dietary proteins and in the process of blood meal digestion. In this study, cDNA was constructed using the whole body of Laccotrephes japonensis. The flanking sequences of the 5-and 3-end of this gene were characterized by RACE-PCR. Sequence analysis showed that this gene contained a 963-bp ORF encoding 320 amino acids. The deduced amino acid sequence showed 62% identity with the Creontiades dilutus serine protease, 58% with the Lygus lineolaris trypsin precursor, and 54% with the Triatoma infestans salivary trypsin. To assess the expression of the L. japonensis serine protease (JGsp), the JGsp gene was cloned into a baculovirus transfer vector, pBac-1, and expressed in Sf9 cells (Spodoptera frugiperda). SDS-PAGE and western blot analysis have shown that the JGsp recombinant protein was a monomer with a molecular weight of about 32 kDa. Recombinant JGsp has shown activity in the protease enzyme assay using gelatin as a substrate.

show abstract

Efficacy of a baculovirus pesticide expressing an eclosion hormone gene

Cited by 29 publications

References 31 publications

Baculovirus‐induced tree‐top disease: how extended is the role ofegtas a gene for the extended phenotype?

Baculovirus‐induced tree‐top disease: how extended is the role ofegtas a gene for the extended phenotype?

Insect Neuropeptides

Molecular Cloning, Gene Structure, Expression, and Enzyme Activity of a Serine Protease from Water Scorpion, Laccotrephes japonensis (Hemiptera: Nepidae)

Contact Info

Product

Resources

About