Efficacy assessment of various natural and organic antimicrobials against Escherichia coli O157:H7, Salmonella enteritidis and Listeria monocytogenes

Amin, Dina H.; Abolmaaty, Assem

doi:10.1186/s42269-020-00423-8

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…The ability to utilize benzoate is shared among bacterial strains that are associated with E. huxleyi in the natural environment and in cultures ( Figure 5a ; Green et al, 2015 ; Orata et al, 2016 ; Rosana et al, 2016 ; Vincent et al, 2021 ). Since benzoate can act as an antibacterial compound ( Amin and Abolmaaty, 2020 ; Haque et al, 2005 ), we propose that secretion of benzoate by E. huxleyi can select for bacteria that specialize on this compound and is therefore important for the establishment of a coexistence phase. Similarly, the diatom Asterionellopsis glacialis produces two secondary metabolites that select for specific bacteria and affect their behavioral response ( Shibl et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…4d) (28,(63)(64)(65). Since benzoate can act as an antibacterial compound (70,71), we propose that secretion of benzoate by E. huxleyi can select for bacteria that specialize on this compound and is therefore important for the establishment of a coexistence phase. Similarly, the diatom Asterionellopsis glacialis produces two unique secondary metabolites, that selects for specific bacteria and also affect their behavioral response (72).…”

Section: D7mentioning

confidence: 98%

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Bacterial lifestyle switch in response to algal metabolites

Barak-Gavish

Dassa

Kuhlisch

et al. 2023

eLife

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Unicellular algae, termed phytoplankton, greatly impact the marine environment by serving as the basis of marine food webs and by playing central roles in the biogeochemical cycling of elements. The interactions between phytoplankton and heterotrophic bacteria affect the fitness of both partners. It is becoming increasingly recognized that metabolic exchange determines the nature of such interactions, but the underlying molecular mechanisms remain underexplored. Here, we investigated the molecular and metabolic basis for the bacterial lifestyle switch, from coexistence to pathogenicity, in Sulfitobacter D7 during its interaction with Emiliania huxleyi, a cosmopolitan bloom-forming phytoplankter. To unravel the bacterial lifestyle switch, we analyzed bacterial transcriptomes in response to exudates derived from algae in exponential growth and stationary phase, which supported the Sulfitobacter D7 coexistence and pathogenicity lifestyles, respectively. In pathogenic mode, Sulfitobacter D7 upregulated flagellar motility and diverse transport systems, presumably to maximize assimilation of E. huxleyi-derived metabolites released by algal cells upon cell death. Algal dimethylsulfoniopropionate (DMSP) was a pivotal signaling molecule that mediated the transition between the lifestyles, supporting our previous findings. However, the coexisting and pathogenic lifestyles were evident only in the presence of additional algal metabolites. Specifically, we discovered that algae-produced benzoate promoted the growth of Sulfitobacter D7 and hindered the DMSP-induced lifestyle switch to pathogenicity, demonstrating that benzoate is important for maintaining the coexistence of algae and bacteria. We propose that bacteria can sense the physiological state of the algal host through changes in the metabolic composition, which will determine the bacterial lifestyle during interaction.

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Section: Discussionmentioning

confidence: 99%

Section: D7mentioning

confidence: 98%

Bacterial lifestyle switch in response to algal metabolites

Barak-Gavish

Dassa

Kuhlisch

et al. 2023

eLife

View full text Add to dashboard Cite

show abstract

“…4d) (28, 63–65). Since benzoate can act as an antibacterial compound (70, 71), we propose that secretion of benzoate by E. huxleyi can select for bacteria that specialize on this compound and is therefore important for the establishment of a coexistence phase. Similarly, the diatom Asterionellopsis glacialis produces two unique secondary metabolites, that selects for specific bacteria and also affect their behavioral response (72).…”

Section: Discussionmentioning

confidence: 99%

Bacterial lifestyle switch in response to algal metabolites

Barak-Gavish

Dassa

Kuhlisch

et al. 2022

Preprint

View full text Add to dashboard Cite

Unicellular algae, termed phytoplankton, greatly impact the marine environment by serving as the basis of marine food webs and by playing central roles in biogeochemical cycling of elements. The interactions between phytoplankton and heterotrophic bacteria affect the fitness of both partners. It is becoming increasingly recognized that metabolic exchange determines the nature of such interactions, but the underlying molecular mechanisms remain underexplored. Here, we investigated the molecular and metabolic basis for the bacterial lifestyle switch, from coexistence to pathogenicity, in Sulfitobacter D7 during its interaction with Emiliania huxleyi, a cosmopolitan bloom-forming phytoplankter. To unravel the bacterial lifestyle switch, we profiled bacterial transcriptomes in response to infochemicals derived from algae in exponential and stationary growth, which induced the Sulfitobacter D7 coexistence and pathogenicity lifestyles, respectively. We found that algal dimethylsulfoniopropionate (DMSP) was a pivotal signaling molecule that mediated the transition between the lifestyles. However, the coexisting and pathogenic lifestyles were evident only in the presence of additional algal metabolites. In the pathogenic mode, Sulfitobacter D7 upregulated flagellar motility and many transport systems, presumably to maximize assimilation of E. huxleyi-derived metabolites released by algal cells upon cell death. Specifically, we discovered that algae-produced benzoate promoted the growth of Sulfitobacter D7, and negated the DMSP-inducing lifestyle switch to pathogenicity, demonstrating that benzoate is important for maintaining the coexistence of algae and bacteria. We propose that bacteria can sense the physiological status of the algal host through changes in the metabolic composition, which will determine the bacterial lifestyle during the interactions.

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