Efficacy and phosphorus equivalency values of two bacterial phytases (Escherichia coli and Citrobacter braakii) allow the partial reduction of dicalcium phosphate added to the diets of broiler chickens from 1 to 21 days of age

Ribeiro, Valdir; Salguero, S. C.; Gomes, Gilson Alexandre; Barros, V.R.S.M.; Silva, D.L.; Barreto, S.L.T.; Rostagno, Horácio Santiago; Hannas, Melissa Izabel; Albino, L. F. T.

doi:10.1016/j.anifeedsci.2016.09.008

Cited by 15 publications

(12 citation statements)

References 26 publications

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“…The interaction between the two lines over the 0–7-day period suggests that this unselected Fayoumi line may be less susceptible to the anti-nutritional effects of phytate or at least less responsive to the high dietary concentrations of phytase. The current data are in agreement with previous reports that high phytase feeding (1500–7500 FTU/kg) resulted in improved performance as either increased weight gain or improved FCR and phytate degradation (Persia 2010; dos Santos et al 2013; Ribeiro et al 2016; Truong et al 2017). Pirgozliev & Bedford (2013) showed that phytase supplementation increased net energy in a linear fashion with 2500 FTU/kg phytase resulting in a 153 kcal/kg increase in net energy over a control diet.…”

Section: Discussionsupporting

confidence: 93%

Differential growth performance and intestinal immune gene expression in diverse genetic lines of growing chickens fed a high concentration of supplemental phytase

2018

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The objective of the current experiment was to determine the effects of high-concentration phytase (5000 FTU/kg) feeding to diverse lines of chickens fed phosphorus (P) adequate maize–soybean meal diets (4.5 g/kg non-phytate P) on the performance and intestinal immune function. Performance was measured for outbred broiler (Ross 308) and inbred Fayoumi lines over 0–21 days, and duodenum and ileum were harvested for the determination of mucin-2, interleukin (IL)-1β and IgA mRNA by quantitative reverse transcription polymerase chain reaction. Over the 0–7-day period, there was a significant line × diet interaction, as high phytase supplementation increased broiler average daily gain (ADG), but had no effect on Fayoumi ADG. Treatment of diets with phytase increased expression of the mucin-2 gene in the duodenum mucosa. There were significant interactions between line and age, and line, diet and age on duodenal expression of the IL-1β gene as phytase supplementation of the broiler line reduced IL-1β in comparison to control fed broilers without change in the Fayoumi line. Overall, the addition of a high concentration of phytase to broilers fed adequate concentrations of non-phytate P resulted in improved growth performance early with a reduction in this effect over time. Mucosal mucin-2 expression was increased with high-concentration phytase feeding across both lines, but IL-1β mRNA expression was reduced in the duodenum of broilers fed high concentrations of phytase, suggesting that the increased performance noted might be related to decreased inflammation.

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Section: Discussionsupporting

confidence: 93%

Differential growth performance and intestinal immune gene expression in diverse genetic lines of growing chickens fed a high concentration of supplemental phytase

2018

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“…phytase or phytase + enzyme complex, their WG did not change relative to that of the birds in the control treatment, and they recovered a part of the lost performance. The results of this study are consistent with those of Dessimoni et al (2019) who also observed an improvement in performance after adding phytase to diets deficient in P. Likewise, Ribeiro et al (2016) showed a positive effect on FI and WG of phytase addition to P-deficient diets. Walk, Pirgozliev, Juntunen, Paloheimo and Ledoux (2018) found that the addition of phytase, phytase + xylanase, or the combination of phytase + xylanase + protease to diets deficient in P and Ca restored FI and WG and improved the feeding efficiency of birds.…”

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confidence: 92%

Broiler feed formulated with phosphorus, calcium, and energy deficiencies supplemented with exogenous enzymes

Jacob

Albino

Calderano

et al. 2021

Semina: Ciênc. Agrár.

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Three experiments were carried out to verify the effects of the enzyme phytase, alone or combined with an enzyme complex, in diets deficient in available phosphorus (AP), calcium (Ca), and metabolizable energy (ME) on broiler performance, ME, and dietary amino acid digestibility. A total of 1,538 male Cobb 500 broilers were allocated to the three experiments, each of which consisted of five treatments: positive control (PC; basal ration); negative control 1 (NC1; PC minus 0.15% of AP, 0.16% of Ca, and 68 kcal kg-1 ME); negative control 2 (NC2; PC minus 0.15% of AP, 0.16% of Ca, and 101 kcal kg-1 of ME); NC1 plus phytase; and NC2 plus phytase plus enzymatic complex. Body weight gain (WG) and feed intake were measured from 1-21 days and from 1-42 days, and the corrected feed conversion rate (FCR) for mortality was calculated. In the second and third experiments, the apparent ME corrected for nitrogen balance (AMEn) and standardized digestibility of amino acids, respectively, were determined, for the diets supplemented with phytase and the enzymatic complex. In the first experiment, enzyme supplementation increased (p < 0.05) WG at 21 days and 42 days relative to the negative controls. Phytase inclusion improved (p < 0.05) FCR at the initial phase compared to the NC1 diet. In the second experiment, enzyme supplementation did not affect (p > 0.05) AMEn. In the third experiment, both enzyme treatments improved (p < 0.05) the digestibility of amino acids in the supplemented diets compared to the deficient diets. Supplementation with phytase and carbohydrases preserves the performance of broilers fed diets deficient in AP, Ca, and ME and improves amino acid digestibility.

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“…Using eggshell thickness as the response parameter resulted in higher P equivalent values compared to other response criteria in methods CC and CC-BD but not in CNP. Estimated P equivalences got from three different methods is a little higher than the values of some other studies performed on laying hens (Simons and Versteegh, 1992; 1993; Waldroup, 1999), which could be attributed to the difference in the method of determination and experimental assays (digestibility trails vs performance trails) (Dersjant-Li et al, 2019) or different adopted response criteria (Adedokun et al, 2004), diet ingredients (Francesch et al, 2005), phytase type (Igbasan et al, 2000; Selle and Ravindran, 2007; Ribeiro et al, 2016), phosphorus source (Li et al, 2015), age of examination (Bedford and Cowieson, 2020) and protein and energy effect of phytase (Ravindran et al, 1999; 2000;Nahm, 2002; Liu et al, 2009). The later item needs more attention when interpreting the P equivalence of phytase.…”

Section: Resultsmentioning

confidence: 57%

Does estimation methods affect on phosphorus equivalence value of phytase for layers and broiler chickens?

Yousefi

Zaghari

2020

Preprint

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Two experiments were performed for evaluating calibration curve (CC) and comparing negative and positive controls (CNP) as a major method for estimating of phytase phosphorus equivalence for layer and broiler chickens. In the first and second experiments, 360 Hy-line W-36 layer hens and 525 day-old Ross-308 broiler chickens were used in a complete randomized design, respectively. Evaluated methods were setting the two regression equations for NPP-supplemented and phytase supplemented treatments with two sub-methods, include calibration curve (CC) or exclude the amount of phosphorus content of basal diet (CC-BD) in calculation, and exploring enzyme equivalency by comparing phosphorus deficient diet as an negative and supplemented diet by inorganic phosphorus sources as a positive control group (CNP). Experiment one included nine treatments (200, 300, 400 and 500 FTU/kg phytase was added to a phosphorus deficient basal diet contained 0.12% Av.P, the rest four treatments were included basal diet supplemented with 0.20, 0.27, 0.35 and 0.43% Av.P). Experiment two included seven treatments (a basal P deficient diet contained 0.27% Av.P, and two increasing levels of Av.P, 0.32 and 0.37%, and four doses of phytase 200, 300, 400 and 500 FTU/kg added to basal diet). Each treatment in the both experiments replicated five times. Results indicated that methods of estimation had a significant effect on phosphorus equivalence estimation (P<0.0001). Fitted regression equations considering P content of basal diet (CC-BD) estimated rational values than those ignore it (CC) (0.161% vs 0.424% and 0.432% vs 0.564% for 500 FTU/kg phytase for broiler chicken and layer hens, respectively) (P<0.0001). On average, among three methods used, CC method had the highest estimated values both in broiler chickens and layer hens (p<0.0001). Regardless of mathematical method, there were different significant values for different strains (layer, 0.381% and broiler, 0.212%) (P<0.0001), but not for different traits served as response criteria (P>0.05). In conclusion, the phosphorus equivalent value of enzyme varies according to the estimation methods and strain. Hence, using matrix values of enzyme for accurate feed formulation depend on a variety of circumstances and decision making requires comprehensive information.

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Efficacy and phosphorus equivalency values of two bacterial phytases (Escherichia coli and Citrobacter braakii) allow the partial reduction of dicalcium phosphate added to the diets of broiler chickens from 1 to 21 days of age

Cited by 15 publications

References 26 publications

Differential growth performance and intestinal immune gene expression in diverse genetic lines of growing chickens fed a high concentration of supplemental phytase

Differential growth performance and intestinal immune gene expression in diverse genetic lines of growing chickens fed a high concentration of supplemental phytase

Broiler feed formulated with phosphorus, calcium, and energy deficiencies supplemented with exogenous enzymes

Does estimation methods affect on phosphorus equivalence value of phytase for layers and broiler chickens?

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