Efficacy and biosafety assessment of neuropeptide CAPA analogues against the peach‐potato aphid (<i>Myzus persicae</i>)

Shi, Yan; Nachman, Ronald J.; Gui, Shun‐Hua; Piot, Niels; Kaczmarek, Krzysztof; Zabrocki, Janusz; Dow, Julian A. T.; Davies, Shireen-A.; Smagghe, Guy

doi:10.1111/1744-7917.12951

Cited by 8 publications

(25 citation statements)

References 36 publications

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“…Neuropeptides regulate a wide array of behavior and physiological processes in arthropods, including feeding, molting, courtship, social interaction, and development. They play their role by binding as ligands with cognate G protein-coupled receptors (GPCRs), which initiates the signaling process [ 1 , 2 ]. Feeding is a complex behavior present in all species, and it is modulated by environmental clues and internal processes.…”

Section: Introductionmentioning

confidence: 99%

Short Neuropeptide F and Its Receptor Regulate Feeding Behavior in Pea Aphid (Acyrthosiphon pisum)

Amir

Shi

Cao

et al. 2022

Insects

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Insect short neuropeptide F (sNPF), an ortholog of prolactin-releasing peptide of invertebrates, regulates diverse biological processes, including feeding, olfaction, locomotion, and sleep homeostasis in insects. However, its function is still unclear in an important model insect and agricultural pest, the pea aphid (Acyrthosiphon pisum). Here, we investigated short neuropeptide F (ApsNPF) and its receptor (ApsNPFR) in A. pisum. The sNPF gene contains three exons and two long introns. In addition, the genome contains a single sNPF receptor with seven transmembrane domains. Stage- and tissue-specific transcript profiling by qRT-PCR revealed that ApsNPF and ApsNPFR were mainly expressed in the central nervous system. The receptor was also detected in antennae, midgut, and integument. The highest expression levels were found in first instar nymphs compared to other developmental stages. Besides, the starvation-induced pattern indicated that the sNPF network depends on the nutritional state of the insect. An electrical penetration graph showed that probing time and phloem duration of A. pisum on broad bean plants decreased in response to dssNPF and dssNPFR in RNAi assays. sNPF silencing reduced the number of nymphs per female but not aphid survival. We believe that our results advance in-depth knowledge of the sNPF/sNPFR signaling cascade and its place in regulating feeding behavior in insects. In turn, it may contribute to the potential design of new strategies to control aphids, with a focus on the sNPF system.

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Section: Introductionmentioning

confidence: 99%

Short Neuropeptide F and Its Receptor Regulate Feeding Behavior in Pea Aphid (Acyrthosiphon pisum)

Amir

Shi

Cao

et al. 2022

Insects

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“…[3][4][5] The use of insect neuropeptides to disrupt physiological processes has been considered as a viable basis for the development of alternative strategies for pest control. 6,7 Due to their high specificity, neuropeptide analogs interacting with the insect neuropeptide signaling systems may represent promising strategies, minimizing detrimental environmental impact. Insecticidal effects of insect neuropeptide analogs, including tachykininrelated peptides (TRP), pyrokinins (PK), myosuppressins (MS) and Capability/CAP2b (CAPA) has been previously demonstrated.…”

Section: Introductionmentioning

confidence: 99%

“…Insecticidal effects of insect neuropeptide analogs, including tachykininrelated peptides (TRP), pyrokinins (PK), myosuppressins (MS) and Capability/CAP2b (CAPA) has been previously demonstrated. [6][7][8][9][10][11][12] Adipokinetic hormone (AKH) was one of the first insect peptides to be isolated, sequenced and synthesized. 13 In insects, AKH is primarily known for its role in the mobilization of lipids, carbohydrates and proline from storage during locomotion, reproduction, development and stress.…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies, the topical exposure or oral delivery of analogs of each of the neuropeptide classes represented in this study to aphids, caused a high aphicide effect. [7][8][9]12,32 However, the impact of combinations of different analogs representing different neuropeptide classes on fitness and mortality of aphids has not been fully established. Based on recent previous research, topical exposure of aphids with the CAPA analog 1895 causes higher mortality compared with the control within 5 days post-treatment.…”

Section: Introductionmentioning

confidence: 99%

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Insecticidal efficacy and risk assessment of different neuropeptide analog combinations against the peach‐potato aphid following topical exposure

Shi

Nachman

et al. 2022

Pest Management Science

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Background: Insect neuropeptides control essential physiological metabolic activities. In our previous studies, Capability/ CAP2b (PK/CAPA) analog 1895 applied alone or as a combination of CAPA analogs (1895 + 2315) was reported to decrease aphid fitness. While this was obtained with the combination of two peptide analogs of the same neuropeptide class, the effect of combining peptide analogs of different neuropeptide classes has not been explored so far.Results: In this study, we assessed the effect of combinations of the PK/CAPA analog 1895 with neuropeptide analogs of four different classes [adipokinetic hormone (AKH) analog: 2271; myosuppressin analog: 2434; kinin analog: 2460; tachykininrelated peptide analog: 2463] on the fitness of aphids. We found that the combination of 1895 and AKH analog 2271 was the most effective one to control Myzus persicae. The triple combination 1895 + 2271 + 2315 provided a synergistic effect by further increasing aphid mortality and reducing reproduction relative to 1895 + 2315. Additionally, a biosafety assessment of the combination 1895 + 2271 + 2315 showed no significant lethal nor sub-lethal effects on survival rates and food intake for the pollinator (Bombus terrestris) and the two representative natural enemies (Harmonia axyridis and Nasonia vitripennis).Conclusion: These results could facilitate establishment of the triple combination 1895 + 2271 + 2315, and/or inclusion of second generation analogs, as alternatives to broad spectrum and less friendly insecticides.

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“…Silencing PTTH receptor torso in the prothoracic gland prolongs the third-instar larval stage of D. melanogaster [16]. Topical application of 1895 and CAPA-PVK analogue 2315 (ASG-[β 3 L]-VAFPRVamide) cause 72% mortality in Myzus persicae, while having no influence on beneficial insects including Bombus terrestris, Chrysoperla carnea, Nasonia vitripennis and Adalia bipunctata [22]. Due to the critical role of neuropeptides and their receptors in insect survival, they have been considered as attractive targets for developing new environmentally friendly pesticides to control the economically important pests [5,[23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi

Xue

Gao

et al. 2021

Insects

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In insects, neuropeptides and their receptors not only play a critical role in insect physiology and behavior but also are the potential targets for novel pesticide discoveries. Aphidius gifuensis is one of the most important and widespread aphid parasitoids, and has been successfully used to control aphid. In the present work, we systematically identified neuropeptides and their receptors from the genome and head transcriptome of A. gifuensis. A total of 35 neuropeptide precursors and 49 corresponding receptors were identified. The phylogenetic analyses demonstrated that 35 of these receptors belong to family-A, four belong to family-B, two belong to leucine-rich repeat-containing GPCRs, four belong to receptor guanylyl cyclases, and four belong to receptor tyrosine kinases. Oral ingestion of imidacloprid significantly up-regulated five neuropeptide precursors and four receptors whereas three neuropeptide precursors and eight receptors were significantly down-regulated, which indicated that these neuropeptides and their receptors are potential targets of some commercial insecticides. The RT-qPCR results showed that dopamine receptor 1, dopamine receptor 2, octopamine receptor, allatostatin-A receptor, neuropeptides capa receptor, SIFamide receptor, FMRFamide receptor, tyramine receptor and short neuropeptide F predominantly were expressed in the head whilst the expression of ion transport peptide showed widespread distribution in various tissues. The high expression levels of these genes suggest their important roles in the central nervous system. Taken together, our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in the regulation of the physiology and behavior of solitary wasps. Furthermore, this information could also aid in the design and discovery of specific and environment-friendly insecticides.

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Efficacy and biosafety assessment of neuropeptide CAPA analogues against the peach‐potato aphid (Myzus persicae)

Cited by 8 publications

References 36 publications

Short Neuropeptide F and Its Receptor Regulate Feeding Behavior in Pea Aphid (Acyrthosiphon pisum)

Short Neuropeptide F and Its Receptor Regulate Feeding Behavior in Pea Aphid (Acyrthosiphon pisum)

Insecticidal efficacy and risk assessment of different neuropeptide analog combinations against the peach‐potato aphid following topical exposure

Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi

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