Efficacy and antiparasitic mechanism of curdione from Curcuma zedoaria against Gyrodactylus kobayashii in goldfish

Zhang, Yilin; Tan, Xiaoping; Tu, Xiao; Ling, Fei; Wang, Gao‐Xue

doi:10.1016/j.aquaculture.2020.735186

Cited by 11 publications

(7 citation statements)

References 53 publications

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“…The use of SEM to determine the normal appearance of D. cycloancistrium was important to identify the changes caused by in vitro exposure to the different products tested. Contrary to the normal D. cycloancistrium characteristics, healthy Gyrodactylus kobayashii , a monogenean of goldfish Carassius auratus , exhibits a smooth cuticle and numerous microvilli on the body surface (Zhang et al, 2020). When treated with Curcuma zedoaria , G. kobayashii demonstrated marked cuticle changes associated with the exposure time (Zhang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Contrary to the normal D. cycloancistrium characteristics, healthy Gyrodactylus kobayashii , a monogenean of goldfish Carassius auratus , exhibits a smooth cuticle and numerous microvilli on the body surface (Zhang et al, 2020). When treated with Curcuma zedoaria , G. kobayashii demonstrated marked cuticle changes associated with the exposure time (Zhang et al, 2020). The integument is the primary surface for the protection and maintenance of homeostasis by monogenean parasites and is essential for their survival (Bakke et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The integument is the primary surface for the protection and maintenance of homeostasis by monogenean parasites and is essential for their survival (Bakke et al, 2007). Thus, dysfunction induced by antiparasitic agents makes the parasite more easily accessible to the fish immune system (Zhang et al, 2020). Thus, according to our results, the products caused tissue damage in D .…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of chlorine, sodium chloride and trichlorfon baths against monogenean Dawestrema cycloancistrium parasite of pirarucu Arapaima gigas

Araújo¹,

Maciel

Costa-Fernandes

et al. 2022

Journal of Fish Diseases

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The pirarucu (Arapaima gigas) is an osteoglossiform fish of the Arapaimidae family and is found naturally in the Amazon Basin and Araguaia River. This species has attributes for aquaculture production, such as fast growth, hardiness, obligatory aerial respiration, good carcass yield, boneless fillets, and high market value (Pereira-Filho et al., 2020;Valladão et al., 2016). The breeding of this species is primarily developed in countries with tropical climates. However, the feasibility of its cultivation has also been studied in countries with temperate climates (Lawson et al., 2015;Lima et al., 2018).The arapaima aquaculture chain is based on three segments: the production of juveniles through natural reproduction, farmers who perform fattening for marketing purposes, and fish processing industries (Lima et al., 2015(Lima et al., , 2017. The larviculture and fry stages are associated with the emergence of diseases caused by parasitic agents as the larvae are collected in breeding ponds and become contaminated by horizontal transmission (Lima et al., 2015(Lima et al., , 2017. Furthermore, juveniles are subjected to confinement during feeding training, a condition that predisposes them to an imbalance in the parasite/host/environment relationship, increasing the prolif-

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Efficacy of chlorine, sodium chloride and trichlorfon baths against monogenean Dawestrema cycloancistrium parasite of pirarucu Arapaima gigas

Araújo¹,

Maciel

Costa-Fernandes

et al. 2022

Journal of Fish Diseases

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“…To determine whether dioscin caused morphological alterations to G. kobayashii tegument, SEM analysis of gyrodactylids samples was conducted following standard methods (30). In short, G. kobayashii-infected goldfish were exposed to 0.06% DMSO (control group) and 0.29 mg/L dioscin (24 h-EC 50 ) for 12 h. Caudal fins with gyrodactylids were clipped and fixed in 2.5% glutaraldehyde solution for 24 h at 4 • C, and then dehydrated by an alcohol gradient, dried with carbon dioxide and sputtercoated with gold particles.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Antiparasitic Efficacy of Herbal Extracts and Active Compound Against Gyrodactylus kobayashii in Carassius auratus

et al. 2021

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Gyrodactylus spp. Nordmann, 1832 (Monogenea: Gyrodactylidae) are common ectoparasites of teleost fishes. Infection with these parasites can increase the mortality of fish and cause considerable economic losses in intensive aquaculture. To find an effective antiparasitic agent for the control of gyrodactylosis, antiparasitic efficacy of crude extracts of 36 herbal medicines was evaluated using a Carassius auratus (Cypriniformes, Cyprinidae)—Gyrodactylus kobayashii model. Among all tested medicines, methanol extract of Dioscorea collettii var. hypoglauca (Dioscoreales, Dioscoreaceae) was the most efficient, with an EC50 value of 4.17 mg/L. This extract showed 100% antiparasitic efficacy against G. kobayashii at 10 mg/L and had a therapeutic index (TI, LC50/EC50) of 5.26, which is higher than that of formaldehyde (TI = 4.58), a widely used parasiticide in aquaculture. Subsequently, the potential mechanism of antiparasitic activity of dioscin, an active compound isolated from D. collettii var. hypoglauca was investigated and the histopathological alterations in goldfish after exposure to dioscin were also studied. The in vivo trial indicated dioscin showed significant antiparasitic activity with a 24 h-EC50 value of 1.58 mg/L and it exhibited 100% antiparasitic efficacy at 0.6 mg/L. Also, G. kobayashii could be completely removed in vivo within 2 h at 0.6 mg/L dioscin. Whereas, mean survival time of this worm in vitro was 4.99 h, and some individuals even reached 12 h at the same concentration of dioscin. These results indicated that 0.6 mg/L of dioscin did not completely kill all worms within 2 h, but just temporarily remove the worms from goldfish. Scanning electron microscopy (SEM) analysis showed that most of the microvilli on the tegument surface of G. kobayashii dropped after exposure to dioscin. This might be one of the potential mechanisms of antiparasitic activity of dioscin against G. kobayashii. Furthermore, no severe histopathological alteration was observed after exposure to a high concentration of dioscin for a short time. Considering both effectiveness and safety, therapeutic baths with a high concentration of dioscin for a short time might be a more optimal choice for the treatment of gyrodactylosis in aquaculture.

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“…Two diterpenes, curcuzedoalide and curcuminol D, were obtained from C. zedoaria cultivated in South Korea [7]. Curdione and curcumol belonging to sesquiterpenes had been isolated previously from essential oils of Chinese C. zedoaria rhizomes [8]. From methanol extract of C. zedoaria rhizomes, purchased from Kyoungdong Herbal Market in Seoul, five sesquiterpenes had been reported such as isoprocurcumenol, germacrone, curzerenone, curcumenol, and curcuzedoalide [9].…”

Section: Introductionmentioning

confidence: 99%

Phenolic Compounds From Indonesian White Turmeric (Curcuma Zedoaria) Rhizomes

Rahayu¹,

Setyani²,

Dianhar³

et al. 2020

Asian J Pharm Clin Res

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Objective: The aim of the present study is to isolate phenolic compounds from Curcuma zedoaria rhizomes grown in Bogor, West Java, Indonesia, which will enrich phytochemical information from this plant. Methods: C. zedoaria rhizomes were macerated in methanol then followed by increasing polarity partitions with n-hexane, ethyl acetate (EtOAc), and methanol, respectively. EtOAc fraction was further fractionated using various chromatography techniques to yield two isolated fractions, Z1 and Z2. These two isolated fractions were then characterized to determine their compound structures. Results: According to Fourier transform-infrared, ultraviolet-vis, and liquid chromatography–mass spectrometry (MS)/MS data, Z1 fraction was elucidated as curcuminoid derivative, that is, dimethoxycurcumin (DiMC, 1), while Z2 fraction was yielded as a mixture consisted of flavonoid and coumarin derivatives, that is, 3,5,7-trihydroxy-4’-methoxyflavon (kaempferide, 2) and 7-methoxy coumarin (herniarin, 3). Conclusion: This study reveals useful information regarding phenolic constituents of Indonesian C. zedoaria rhizomes. Further research needs to be carried out to purify other compounds contained and to conduct bioactivity assays.

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Efficacy and antiparasitic mechanism of curdione from Curcuma zedoaria against Gyrodactylus kobayashii in goldfish

Cited by 11 publications

References 53 publications

Efficacy of chlorine, sodium chloride and trichlorfon baths against monogenean Dawestrema cycloancistrium parasite of pirarucu Arapaima gigas

Efficacy of chlorine, sodium chloride and trichlorfon baths against monogenean Dawestrema cycloancistrium parasite of pirarucu Arapaima gigas

Antiparasitic Efficacy of Herbal Extracts and Active Compound Against Gyrodactylus kobayashii in Carassius auratus

Phenolic Compounds From Indonesian White Turmeric (Curcuma Zedoaria) Rhizomes

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