Efficacies of Various Anaerobic Starter Seeds for Biogas Production from Different Types of Wastewater

Abstract

Various anaerobic starter seeds from different sources were investigated for their efficacies in treatment of different types of wastewater. Six combinations of starter seeds and wastewaters were selected out of 25 combination batch experiments and operated in semicontinuous reactors. It was noticed that the efficacies of various anaerobic starter seeds for biogas production from different types of wastewater in terms of reactor performance and stability were depended on wastewater characteristics and F/M ratio affecting microbial community and their microbial activities. However, exogenous starter seed can be used across different types of wastewater with or without acclimatization. Four reactors reached the targeted OLR of 2 kg COD/m3·d with high performance and stability except for concentrated rubber wastewater (RBw), even using high active starter seeds of cassava starch (CSs) and palm oil (POs). The toxic compounds in RBw such as ammonia and sulfate might also adversely affect methanogenic activity in CSsRBw and POsRBw reactors. DGGE analysis showed that propionate utilizers, Smithella propionica strain LYP and Syntrophus sp., were detected in all samples. For Archaea domain, methylotrophic, hydrogenotrophic, and acetoclastic methanogens were also detected. Syntrophic relationships were assumed between propionate utilizers and methanogens as acetate/H2 producers and utilizers, respectively.

Diverse Microbial Community Profiles of Propionate-Degrading Cultures Derived from Different Sludge Sources of Anaerobic Wastewater Treatment Plants

“…The total reactions produced more acetate molecules compared to the classical pathway which belongs to Syntrophobacter and Pelotomaculum [23]. Following this theoretical perspective, we observed a higher abundance of Methanosaeta, which produces methane by acetate degradation, in the enriched samples [46]. Furthermore, Syntrophomonas was observed in several enriched samples.…”

Study on Co-Digestion of Palm Oil Mill Effluent and Empty Fruit Bunches to Improve Biogas Production in Palm Oil Mill

“…In this research, sludge of anaerobic bacteria was collected from commercial biogas plant with utilized tapioca wastewater as a feedstock. Tapioca wastewater has a high carbohydrate content, namely around 13,970 mg/L, total solids around 19,370 mg/L, and volatile solids around 16,910 mg/L (Chaiprasert et al, 2017). Because of POME has different characteristics with tapioca wastewater, acclimatization using POME is very important.…”