Effets du cadmium sur l'accumulation ionique et les teneurs en lipides dans les feuilles de tomate (<i>Lycopersicum esculentum</i>)

Ammar, Wided Ben; Nouairi, Issam; Tray, B.; Zarrouk, Mokhtar; Jemal, Fatma; Ghorbel, Mohamed Habib

doi:10.1051/jbio:2005016

Cited by 15 publications

(9 citation statements)

References 24 publications

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“…Studies carried out in different plant species have revealed that Cd causes growth inhibition (Scebba et al 2006, Agrawal et al 2006) and even plant death. It is well established that photosynthesis , mineral nutrition, membrane structure, etc., are affected by the presence of Cd (Ben Ammar et al 2005). Besides, Cd induced peroxidation of membrane lipids by generating reactive oxygen species (ROS) (Dixit et al 2001, Ben Youssef et al 2005.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

“…However, roots growth was damaged with the highest cadmium concentrations. The reduction in growth could be a consequence of the Cd-interference with a number of metabolic processes associated with normal development such as mineral nutrition (Drazic et al 2006), membrane lipid composition (Ben Ammar et al 2005) and photosynthetics pigments production ( Table 1). The low sensitivity of roots to Cd could be explained by their capacity to accumulate the metal in a non-active form, fixed to the carboxyl (Clemens 2001) and/or sulphhydryl groups (Cobbett 2000).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

“…We have noted that Cd reduced the contents of chloroplastic and extrachloroplastic lipids. Likewise, levels of tri-unsaturated fatty acids: linolenic (C18:3) and hexadecatrienoïc (C16:3) dropped in membrane lipids (Ben Ammar et al 2005).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

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Antioxidative response to cadmium in roots and leaves of tomato plants

Ammar¹,

Nouairi²,

Zarrouk³

et al. 2008

Biologia plant.

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Treatment of tomato seedlings (Lycopersicon esculentum Mill. cv. 63/5 F1) with increasing CdCl 2 concentrations in the culture medium resulted in Cd accumulation more important in roots than in leaves. Biomass production was severely inhibited, even at low Cd concentration. Cd reduced chlorophyll content in leaves and enhanced lipid peroxidation. An increase in antioxidative enzyme (superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase) activities was more pronounced in leaves than in roots, while catalase activity increased only in roots. In addition, changes in isoenzyme composition were observed using the non-denaturing polyacrylamid gel electrophoresis.Additional key words: lipid peroxidation, Lycopersicon esculentum, oxidative stress.

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Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

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Antioxidative response to cadmium in roots and leaves of tomato plants

Ammar¹,

Nouairi²,

Zarrouk³

et al. 2008

Biologia plant.

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“…Cadmium is a non-redox-reactive heavy metal, and its toxicity is believed to be due to its action on a wide range of plant cellular activities. In non-tolerant plant species, cadmium has been shown to affect the mineral distribution , the nitrogen (Chaffei et al 2003) and carbon assimilation , the photosynthetic processes (Fargašová 2004;Ben Ammar et al 2005) and the enzymatic activity (Schützendübel et al 2001;Sobkowiak et al 2004). Many of these effects can be interrelated through a general action on membrane biogenesis and integrity, which in turn can occur because lipid metabolism is altered.…”

Section: Introductionmentioning

confidence: 99%

The effect of cadmium on lipid and fatty acid biosynthesis in tomato leaves

et al. 2008

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Abstract:This research aims to examine the effect of cadmium uptake on lipid composition and fatty acid biosynthesis, in young leaves of tomato treated seedlings (Lycopersicon esculentum cv. Ibiza F1). Results in membrane lipids investigations revealed that high cadmium concentrations affect the main lipid classes, leading to strong changes in their composition and fatty acid content. Thus, the exposure of tomato plants to cadmium caused a concentration-related decrease in the unsaturated fatty acid content, resulting in a lower degree of fatty acid unsaturation. The level of lipid peroxides was significantly enhanced at high Cd concentrations. Studies of the lipid metabolism using radioactive labelling with [1-14 C]acetate as a major precursor of lipid biosynthesis, showed that levels of radioactivity incorporation in total lipids as well as in all lipid classes were lowered by Cd doses. In total lipid fatty acids, [1-14 C]acetate incorporation was reduced in tri-unsaturated fatty acids (C16:3 and C18:3); While it was enhanced in the palmitic (C16:0), palmitoleic (C16:1), stearic (C18:0) and linoleic (C18:2) acids. [1-14 C]acetate incorporation into C16:3 and C18:3 of galactolipids [monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG)] and some phospholipids [phosphatidylcholine (PC) and phosphatidylglycerol (PG)] was inhibited by Cd stress. Our results showed that in tomato plants, cadmium stress provoked an inhibition of polar lipid biosynthesis and reduced fatty acid desaturation process.

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“…It has been shown that Cd toxicity is a major factor limiting plant growth in many soils (Artetxe et al 2002;Fediuc and Erdei 2002;Sandalio et al 2001). Cd affects a wide range of cellular activities, such as mineral distribution (Ben Ammar et al 2005), nitrogen (Chaffei et al 2003) and carbon assimilations, photosynthetic processes (Fargašová 2004;Ben Ammar et al 2005) and enzyme activities (Schützendübel et al 2001;Sobkowiak et al 2004). Some of these effects are mediated through a general action on the membrane structure and behaviour, resulting from an alteration in lipid composition.…”

Section: Introductionmentioning

confidence: 99%

Cadmium stress induces changes in the lipid composition and biosynthesis in tomato (Lycopersicon esculentum Mill.) leaves

Ammar¹,

Nouairi

Zarrouk

et al. 2007

Plant Growth Regul

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The effects of cadmium (Cd) stress on lipid composition and biosynthesis were investigated in young leaves of ten-day-old tomato seedlings (Lycopersicon esculentum Mill. cv. Ibiza F1). Cd was found to be mainly accumulated in roots, but a severe inhibition of biomass production occurred in leaves, even at its low concentration (1.0 lM). Seven days after Cd treatment, the membrane lipids were extracted and separated on silica-gel thin layer chromatography (TLC). Fatty acid methyl esters were analyzed by FID-GC on a capillary column. Our results showed that Cd stress decreased the quantities of all lipids classes (phospholipids, galactolipids and neutral lipids). Likewise, there was also a decline in the levels of tri-unsaturated fatty acids, such as linolenic (C18:3) and hexadecatrienoic (C16:3) acids. The linolenic acid (C18:3) decreased in monogalactosyldiacylglycerol (MGDG) and all phospholipids, while hexadecatrienoinic acid (C16:3) declined mainly in MGDG. Moreover, Cd at high concentrations (25.0 and 50.0 lM) significantly enhanced the levels of lipid peroxides. Radiolabelling experiments were carried out by laying down microdroplets of [1-14 C]acetate-a major precursor of lipid biosynthesis-on attached leaves of the control and Cd-treated plants. After incubation for 1, 2, 12 and 24 h, the leaves were harvested and lipids extracted and analysed. Cd stress was found to decrease the incorporation of [1-14 C]acetate in total lipids. The biosynthesis of total lipids was altered with 25.0 and 50.0 lM Cd. The decline in the incorporation of [1-14 C]acetate due to Cd stress was observed in all lipid classes. There was also a substantial decline in the incorporation of [1-14 C]acetate in tri-unsaturated fatty acids. The results indicate that Cd treatment induces an oxidative stress by inhibiting the chloroplastic and extrachloroplastic lipid-biosynthesis pathways as well as lipid peroxidation.

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Effets du cadmium sur l'accumulation ionique et les teneurs en lipides dans les feuilles de tomate (Lycopersicum esculentum)

Cited by 15 publications

References 24 publications

Antioxidative response to cadmium in roots and leaves of tomato plants

Antioxidative response to cadmium in roots and leaves of tomato plants

The effect of cadmium on lipid and fatty acid biosynthesis in tomato leaves

Cadmium stress induces changes in the lipid composition and biosynthesis in tomato (Lycopersicon esculentum Mill.) leaves

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