The poly2-vinylpyridine and cationic polymer-mixed metal complexes (P2VPMC) have been prepared and characterized by analytical measurement such as: molecular weight, elemental analysis, IR-spectroscopy, NMR-spectroscopy, XRD, and UV. The degree of N-alkylation of poly vinyl pyridine (P-2VP) was 10% determined by chlorine content according to elemental microanalysis. Through ICP instrument the complexation between P-2VPC and the mixed metal chlorides was proven. The structure of the samples was characterized using X-ray diffraction. It was found that the complexes have amorphous properties. The antitumor activity of this compound was examined in vitro on cell lines. The results reflect pronounced cytotoxicity of P-2VPMC against animal experimental EAC cells line, and human, HeLa, HCT116 and Hep-G2, cell lines. Antitumor activity of the novel compound as applied on mice bearing Ehrlich solid carcinoma, revealed delays tumor growth compared with untreated animals and decreases tumor volume. The antitumor activity of the P2VPMC might be due to its ability to pass through the membrane and interact with, DNA causing cross-linking action which might be the key factor for great antitumor activity of the P2VPMC compound.