Effekt von Suramin auf Proliferation und Migration von Linsenepithelzellen in vitro

Rieck, Peter; Kriegsch, J.; Jaeckel, Carsten; Hartmann, Christian

doi:10.1007/s00347-003-0908-x

Cited by 8 publications

(9 citation statements)

References 23 publications

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“…As a prerequisite for this success, the biological effectiveness of drugs regarding the inhibition of LECs was examined in different in vitro [21,22,25,29,30,41] and in vivo animal models, such as in rats [40] , rabbits [24,31,37,46] a Drug dissolved in pure water: differences between TETD-MTX = n.s., TETD-AM = s., and MTX-AM = n.s.…”

Section: Discussionmentioning

confidence: 99%

“…During the 1980s, numerous investigators [21,22] have conducted cell culture studies to examine the potential of pharmacological substances in order to successfully prevent LEC proliferation and migration. Pharmacological agents that have been investigated include cytostatic drugs, such as 5-fluorouracil [23,24] , daunomycin [21] , colchicine, doxorubicin [25] , mitomycin C [23, 26] , and methotrexate (MTX) [27] , anti-inflammatory substances, such as dexamethasone [28] and diclofenac [28,29] , calcium channel blockers, such as mibefradil [30] , and immunological agents, such as cyclosporine A [31] .…”

Section: Introductionmentioning

confidence: 99%

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Drug-Induced Secondary Cataract Prevention: Experimental ex vivo and in vivo Results with Disulfiram, Methotrexate and Actinomycin D

et al. 2010

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Background/Aims: A clinical approach to prevent secondary cataract after lens implantation involves the intraocular application of pharmacological agents. The goals of our study were to develop an ex vivo model to test the drug effectiveness for lens epithelial cell ablation from the basal membrane and to verify the data in rabbit intraocular lens implantation experiments. Methods: Human capsular rhexis specimens were incubated with drugs and the residual cells were differentiated by use of the Live-Dead assay and quantified by staining with Hoechst dye. After phakoemulsification of rabbit eyes and before intraocular lens implantation, capsular bags were filled with drug-loaded hyaluronic acid for 5 min. Results: An ex vivo model was established which allows the testing of drugs on lens epithelial cell ablation. Drug treatment reduced the number of viable cells on the specimens drastically, ranging between 0.44 ± 0.53% (6.0 ± 7.3 cells/mm²) for disulfiram, 0.27 ± 0.50% (3.7 ± 6.9 cells/mm²) for methotrexate and 0.07 ± 0.19% (0.1 ± 0.27 cells/mm²) for actinomycin D. Rabbit eyes treated with a mixture of methotrexate/actinomycin D showed no posterior capsule opacification at 4 months and a low opacification 6 months postoperatively. Without drug treatment low opacification starts 6 weeks postoperatively. Conclusions: The drug screening in the described ex vivo model can help to reduce the number of preclinical studies for secondary cataract prevention. The successful ex vivo cell ablation by methotrexate/actinomycin D was confirmed by a delayed in vivo secondary cataract formation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Drug-Induced Secondary Cataract Prevention: Experimental ex vivo and in vivo Results with Disulfiram, Methotrexate and Actinomycin D

et al. 2010

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“…Rieck und Mitarbeiter [59] untersuchten die Wirkung von Suramin auf bovine und humane Linsenepithelzellen in vitro, welches bereits experimentell zur Reduktion einer proliferativen Retinopathie evaluiert wurde [16]. Suramin besitzt antiproliferative sowie migrationshemmende Eigenschaften durch Inhibition von Wachstumsfaktoren und Enzymen.…”

Section: Weitere Substanzenunclassified

“…Suramin besitzt antiproliferative sowie migrationshemmende Eigenschaften durch Inhibition von Wachstumsfaktoren und Enzymen. Die Autoren konnten eine signifikante Inhibition von Zellproliferation und -migration schon nach kurzer Inkubationszeit nachweisen [59].…”

Section: Weitere Substanzenunclassified

“…Schon 1988/89 veröffentlichte er seine Ergebnisse hinsichtlich Toxizität von Daunomycin auf Linsenepithelzellen [27,28,70]. Darüber hinaus evaluierte er mit seiner Arbeitsgruppe auch Adhäsionshemmstoffe, wie zyklische RGD-Peptide [37], und versuchte, neue Substanzen, wie das Suramin [59], und weitere Mechanismen [6], die für die Nachstarentstehung von Bedeutung sein könnten, aufzuzeigen.…”

Section: Zusammenfassungunclassified

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Pharmakologische Ansätze zur Prävention der Cataracta secundaria

Rabsilber¹,

Auffarth²

2006

Klin Monatsbl Augenheilkd

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Secondary cataract or posterior capsule opacification (PCO) is still the most frequent long-term complication of cataract surgery. Tremendous advances have been made, especially during the last 10 to 15 years, in terms of surgical techniques and improvement of implant technology. However, the problem of proliferation and migration of lens epithelial cells (LECs) postoperatively has not yet been solved completely although we know that a sharp optic edge of intraocular lenses (IOL) combined with hydrodissection, complete overlapping of capsulorhexis and IOL-optic as well as capsular bending reduce PCO formation significantly. In the 1980 s, investigators like Hartmann et al. began with the application of pharmacological substances in-vitro in order to successfully prevent LECs from proliferating and migrating. Cytostatic drugs, steroids, non-steroidal antiphlogistics, adhesion inhibitors, heparin, lidocaine, suramin, immunotoxins, photodynamic therapy and osmotic effective solutions were tested. In several studies different drug delivery systems were investigated in order to provide a longer and more effective impact on LECs. However, the in-vivo use has been viewed critically since the selective targeting of LECs was not possible and serious damage to the surrounding tissue had to be considered. Recently, the development of the PerfectCapsule System for vacuum-sealed capsule irrigation allows the selective targeting of LECs inside the capsular bag. This survey gives an update on past, current and future means and trends to reduce or prevent PCO formation pharmacologically.

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Klassifikation biomedizinischer Forschungsberichte als Grundlage evidenzbasierter Medizin in der Augenheilkunde

et al. 2005

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Effekt von Suramin auf Proliferation und Migration von Linsenepithelzellen in vitro

Cited by 8 publications

References 23 publications

Drug-Induced Secondary Cataract Prevention: Experimental ex vivo and in vivo Results with Disulfiram, Methotrexate and Actinomycin D

Drug-Induced Secondary Cataract Prevention: Experimental ex vivo and in vivo Results with Disulfiram, Methotrexate and Actinomycin D

Pharmakologische Ansätze zur Prävention der Cataracta secundaria

Klassifikation biomedizinischer Forschungsberichte als Grundlage evidenzbasierter Medizin in der Augenheilkunde

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