Effects on Lipid Peroxidation and Antioxidative Enzymes of Euonymus alatus in Cultured Rat Hepatocytes

Abstract

Abstract:The Euonymus alatus (Thunb.) Sieb. has long been used as a crude drug. In this paper, we investigate the effects of E. alatus on cultured hepatocyte cell system and lipid peroxidation in hydrogen peroxide (H 2 O 2 ) treatment conditions. The study covers the physiological activity (the antioxidative activity and the nitrite-scavenging effect) of E. alatus . H 2 O 2 that can produce intracellular free radical was used for inducer of the peroxidation of cellular lipids. Treatment of E. alatus attenuated in cell killing enhanced by increasing concentrations of H 2 O 2 . The increased malondialdehyde level induced by H 2 O 2 treatment was reduced by pre-treatment of E. alatus . Furthermore, addition of E. alatus in cell culture medium significantly reduced cell killing and content of intracellular antioxidants. Changes in nitrite-scavenging effect of E. alatus at various concentrations (5-25 mg/ml) and various pH levels (pH 1.2, 4.2 and 6.0) were also observed. The present study was also done to investigate the effects of E. alatus on cultured hepatocyte cell system, H 2 O 2 -induced cytotoxicity and antioxidative enzyme activities, including catalase, superoxide dismutase, glutathione peroxidase and glutathione S-transferase in H 2 O 2 treatment conditions. E. alatus treatment had significant protective or elevating activities on these antioxidative enzyme activities compared to a normal group. The results indicate that E. alatus provides a strong antioxidant protection of cells against H 2 O 2 -induced oxidative stress.

Effects of Low-Dose versus High-Doseγ-Tocotrienol on the Bone Cells Exposed to the Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis

“…Similar findings were demonstrated with pheochromocytoma cell lines (PC12) [48], HUVEC cells [49], rat hepatocytes [50], bone marrow stromal cells [37], and MC3T3-E1 cells [51]. The antioxidant enzyme was used up to eliminate the lipid peroxidation products or deactivated and glycated by the radicals [52, 53].…”

Cichorium intybus attenuates streptozotocin induced diabetic cardiomyopathy via inhibition of oxidative stress and inflammatory response in rats

“…The cytotoxic action of STZ is allied with the generation of reactive oxygen species causing oxidative damage (Szkudelski, 2001). Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) provides support in scavenging the free radicals by breaking down the ROS (Kim JK et al, 2009). Past investigation have reported a flaw in the scavenging machinery in diabetic patients as compared to the healthy control (Anwer et al, 2012;Anwer et al, 2007).…”

Direct and protective effects of single or combined addition of vincristine and ε-viniferin on human HepG2 cellular oxidative stress markers in vitro

“…Unfortunately, due to the complexity of organ and tissue organization in in vivo experiments, along with sudden hormonal changes and other factors, it is difficult to prove the molecular mechanism of a specific regulation. One of the most commonly used external signals increasing intracellular oxidative stress for an investigation like this one in cultured cells is H 2 O 2 (Alia et al 2005;Fukui et al 2010;Kim et al 2008;O'Brien et al 2000). H 2 O 2 damages DNA, lipids, and other macromolecules in the cell, and may lead to oxidative damage (Aherne and O'Brien 1999).…”