Effects on growth and macromolecular synthesis of starving Neurospora crassa lys 3 of lysine

Fletcher, Margaret H.; Trinci, Anthony P. J.

doi:10.1016/s0007-1536(81)80144-1

Cited by 4 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The dilution scheme for each antifungal agent was within 0.1-100 jxg.ml -~ in a total volume of 20 ml per petri dish. Each petri dish was inoculated with 1 ml mycelial suspension, and then incubated at 28 ° C for 72 h. Dry weight measurements were made as described by Fletcher and Trinci (1981). The MIC was determined as the lowest antifungal concentration that could prevent an increase in dry weight.…”

Section: Methodsmentioning

confidence: 99%

Determination of anti-Aspergillus activity of antifungal agents based on the dynamic growth rate of a single hypha

Matsuoka

Nemoto

et al. 1993

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The dynamic growth rate of a single hypha of Aspergillus niger was analysed using an automatic system. A colony of A. niger was in contact with saline, saline containing an antifungal agent, and flushing saline, in sequence. The growth rate of a test hypha selected arbitrarily from the colony responded dynamically to the antifungal agent. The minimum concentration that caused the complete inhibition of hyphal growth was defined as the minimum inhibitory concentration (MIC). The MIC values obtained were compared with those determined by conventional methods based on increasing rate of colony diameter or dry matter weight.

show abstract

Section: Methodsmentioning

confidence: 99%

Determination of anti-Aspergillus activity of antifungal agents based on the dynamic growth rate of a single hypha

Matsuoka

Nemoto

et al. 1993

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…The amount of fungal biomass is determined by weighing the dry matter of fungi after separating it from a liquid medium (2,8,21). The appearance of colonies on a solid medium is a useful and widely used indicator of whether growth is inhibited (5,11,12).…”

mentioning

confidence: 99%

Evaluation of antifungal volatile compounds on the basis of the elongation rate of a single hypha

Matsuoka

Takekawa

Teraoka

1990

Appl Environ Microbiol

View full text Add to dashboard Cite

A novel method is proposed for the evaluation of the activity of an antifungal agent administered as a gas. This system is composed of a batch-flow type reaction vessel, a gas flow system, and a microscopic observation system. The agar plate was prepared on the ceiling of the reaction vessel, and the mycelium of a fungus (Aspergillus niger or Rhizoctonia solani) was inoculated onto it. After preincubation at 25°C for 24 h, the reaction vessel was connected to the gas flow system. An appropriate hypha was selected, and its elongation rate was measured. Then a sample holder containing an antifungal compound was inserted into the reaction vessel from the side hole to saturate the atmosphere inside with its vapor. The retardation or inhibition of the hypha elongation was observed on a television monitor and recorded on a video tape recorder. The antifungal compound was then removed, and the reaction vessel was flushed with air. If the hypha lived, it began to elongate again. By this method, antifungal activity of seven odor compounds could be evaluated quantitatively within several hours.

show abstract

Influence of antifungal pre-treatment in preparing test mycelium on MIC values of several antifungal agents against Aspergillus niger

et al. 1996

View full text Add to dashboard Cite

Effects on growth and macromolecular synthesis of starving Neurospora crassa lys 3 of lysine

Cited by 4 publications

References 15 publications

Determination of anti-Aspergillus activity of antifungal agents based on the dynamic growth rate of a single hypha

Determination of anti-Aspergillus activity of antifungal agents based on the dynamic growth rate of a single hypha

Evaluation of antifungal volatile compounds on the basis of the elongation rate of a single hypha

Influence of antifungal pre-treatment in preparing test mycelium on MIC values of several antifungal agents against Aspergillus niger

Contact Info

Product

Resources

About