Effects on growth and comparison of root tissue colonization patterns of <i>Eucalyptus viminalis</i> by pathogenic and nonpathogenic strains of <i>Fusarium oxysporum</i>

Salerno, M.; Gianinazzi, Silvio; Gianinazzi‐Pearson, Vivienne

doi:10.1046/j.1469-8137.2000.00629.x

Cited by 30 publications

(34 citation statements)

References 33 publications

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“…This has been observed for Fusarium species on a range of hosts (Alabouvette et al 2009) including woody plants (Marks 1965, Farquhar and Peterson 1989, Rodríguez-Gálvez and Mendgen 1995, Salerno et al 2000. At 42 DAS, when seedlings were packed into the trial design, roots had not yet grown into contact with the seedling container cavity sides (based on six seedlings sampled data not shown here) but by 86 days nine sampled seedlings had all established roots in contact with walls of container cavities.…”

Section: Discussionsupporting

confidence: 53%

Re-use of seedling containers andFusarium circinatumassociation with asymptomaticPinus patulaplanting stock

Morris¹,

Fourie²,

Greyling³

et al. 2014

Southern Forests: a Journal of Forest Science

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Fusarium circinatum is a pathogen causing serious post-planting mortality of Pinus patula seedlings in southern Africa. Containerized planting stock that is asymptomatic but associated with F. circinatum in the nursery is thought to be the cause of this problem. The aim of this study was to determine if re-use of seedling containers could be a source of inoculum resulting in asymptomatic planting stock and increased post-planting mortality of Pinus patula. Two experiments were conducted in successive years comparing nursery cull of symptomatic seedlings, seedling growth, association of F. circinatum with asymptomatic seedlings and post-planting mortality for crops raised in re-used containers, with and without sanitation, and factory new containers. Each experiment consisted of a nursery production trial followed by out-planting into pots to assess post-planting mortality. Our results show that re-use of containers without sanitation increases the cull of symptomatic seedlings, incidence of F. circinatum associated with asymptomatic seedlings and post-planting mortality compared to the re-use of containers after steam sanitation or factory new containers. Growth of asymptomatic seedlings was unaffected by container treatment or association with F. circinatum and in the absence of wilt symptoms the root system did not exhibit typical discolouration. Watering frequency did not influence post-planting mortality in pots. The comparison of two open-pollinated seed mixes of P. patula that, based on seedling stem inoculation screening, represented susceptible and tolerant material did not show differences in nursery cull or post-planting mortality. This work demonstrated that natural contamination of re-used containers can be a primary source of inoculum producing asymptomatic seedlings associated with F. circinatum that will succumb to the pathogen after field planting. The process of seedling infection, apparent latent infection in the seedling and expression of disease after planting needs greater understanding to improve nursery hygiene measures to control this disease.

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Section: Discussionsupporting

confidence: 53%

Re-use of seedling containers andFusarium circinatumassociation with asymptomaticPinus patulaplanting stock

Morris¹,

Fourie²,

Greyling³

et al. 2014

Southern Forests: a Journal of Forest Science

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“…Wounding has been shown to assist fungal penetration into inoculated plant tissues (Yusnita & Sudarsono 2004;Sakamoto & Gordon 2006). Observed wilting symptoms of banana seedling were the results of blocking of xylem tissues by Foc mycelia and by induction of thylosis because of activities of secreted enzymes and fusaric acid by F. oxysporum (Salerno et al 2000). Infected root tissue into brownish or blackish colour could indicate the presence of damages in the root tissue of banana caused by Foc inoculation.…”

Section: Discussionmentioning

confidence: 99%

An Optimized Inoculation Method of Fusarium Wilt (Fusarium oxysporum f. Sp. cubense) Causal Agent of Banana Wilt Disease in Ethiopia

Gabrekiristos¹,

Yesuf²,

Ayana³

2018

GJPBCS

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Banana is among the most important fruit crops in Ethiopia and is produced widely by small scale farmers. Despite its importance and cultivation, its production is, however, hampered by different biotic and abiotic factors. Fusarium wilt of banana caused by soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc) is becoming an important production constraint of the crop. In Ethiopia it was reported in 2014 in potential banana producing areas mainly in Dale, Aleta Chuko and Samen Bench in Southern Nations, Nationalities and Peoples Regional State. The general management option to this disease is replacing susceptible cultivars by resistant/tolerant ones. Use of resistant/tolerant varieties is the most cost-effective and sustainable method of managing the disease. A reliable and rapid screening method is needed to select resistant/tolerant banana varieties. In Ethiopia there is no developed protocol to artificially inoculate banana fusarium wilt in order to screen resistant/tolerant variety. An optimized inoculation methods by root dipping into an adjusted suspension of Foc inoculum concentration 1x10 6 conidia/ml on acclimatized three months old tissue cultured plantlets of susceptible variety (poyo) has enabled to observe typical symptom of FW by tenth days of inoculation and an overall result was obtained within three months. This protocol facilitates the way to produce symptoms of fusarium wilt in banana and screen resistant/tolerant variety released in Ethiopian Research system. Therefore, this paper presents the details of the protocol and achievements of the study in major banana growing areas of Ethiopia.

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“…Salah satu patogen tular tanah yang sering menyerang semai di areal persemaian adalah Fusarium oxysporum. Beberapa semai di persemaian yang dilaporkan terserang patogen ini, diantaranya semai Pinus merkusii (Achmad et al, 1997), P. Pinea (Machón et al, 2009) dan Eucalyptus viminalis (Salerno et al, 2000).…”

Section: Pendahuluanunclassified

Mekanisme Parasitisme Trichoderma Harzianum Terhadap Fusarium Oxysporum Pada Semai Acacia Mangium

Tasik¹,

Widyastuti²,

Harjono³

2015

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Mechanism of parasitism of Trichoderma harzianum on Fusarium oxysporum on Acacia mangium seedlings. Fusarium oxysporum is one of the most important soil-borne fungi the causal agent of damping-off disease. Detailed information it needed to know how the pathogen can be inhibited by Trichoderma harzianum. The objective of this research was to investigate the inhibition mechanism of T. harzianum on F. oxysporum in vitro and in planta. Green Flourescent Protein (GFP) T. harzianum was used as biocontrol agent of F. oxysporum. An in vitro inhibition test of T. harzianum was performed using dual culture method. In the in planta inhibition tests, seedlings of A. mangium were applied with GFP T. harzianum two days before inoculation of F. oxysporum; GFP T. harzianum was simultaneously applied with F. oxysporum and GFP T. harzianum was applied two days after inoculation of F. oxysporum. The inhibition effect of T. harzianum GFP was observed at seven days incubation, indicated by attachment of T. harzianum to F. oxysporum hyphae. GFP T. harzianum hyphae covered the colonies of F. oxysporum at 12 days after incubation. The highest life percentage of A. mangium seedlings was found on the treatment of GFP T. harzianum two days before inoculation of F. oxysporum (82.22%), whereas the lowest life percentage was found on seedling applied with GFP T. harzianum two days after inoculation of F. oxysporum (64.44%). Key words: Acacia mangium, damping-off, Fusarium oxysporum, green fluorescent protein (GFP), Trichoderma harzianum ABSTRAKMekanisme parasitisme Trichoderma harzianum terhadap Fusarium oxysporum pada semai Acacia mangium. Fusarium oxysporum adalah salah satu jamur patogen tular tanah yang penting dan dapat menyebabkan penyakit rebah kecambah. Informasi terperinci mengenai jamur tersebut diperlukan untuk mengetahui bagaimana pertumbuhan jamur tersebut dapat dihambat oleh Trichoderma harzianum. Tujuan dari penelitian ini adalah untuk mengetahui mekanisme penghambatan T. harzianum terhadap F. oxysporum secara in vitro and in planta. Trichoderma harzianum green fluorescent protein (GFP) digunakan sebagai agen pengendali hayati terhadap F. oxysporum. Pengujian secara in vitro menggunakan metode kultur ganda. Susunan perlakuan pada pengujian secara in planta yaitu, aplikasi T. harzianum GFP dua hari sebelum inokulasi F. oxysporum; aplikasi T. harzianum GFP 2 bersamaan dengan inokulasi F. oxysporum; aplikasi T. harzianum GFP dua hari setelah inokulasi F. oxysporum. Pengaruh penghambatan T. harzianum GFP diamati pada tujuh hari setelah inkubasi, indikasinya ialah adanya penempelan hifa T. harzianum GFP pada hifa F. oxysporum. Hifa T. harzianum GFP menutupi koloni F. oxysporum pada 12 hari setelah inkubasi. Rata-rata persentase hidup semai A. mangium tertinggi ditunjukkan pada perlakuan aplikasi T. harzianum GFP dua hari sebelum inokulasi F. oxysporum yaitu sebesar 82,22 %, dan terendah pada perlakuan aplikasi T. harzianum GFP dua hari setelah inokulasi F. oxysporum sebesar 64,44%. Kata kunci: Acacia mangium, Fusarium oxysp...

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Effects on growth and comparison of root tissue colonization patterns of Eucalyptus viminalis by pathogenic and nonpathogenic strains of Fusarium oxysporum

Cited by 30 publications

References 33 publications

Re-use of seedling containers andFusarium circinatumassociation with asymptomaticPinus patulaplanting stock

Re-use of seedling containers andFusarium circinatumassociation with asymptomaticPinus patulaplanting stock

An Optimized Inoculation Method of Fusarium Wilt (Fusarium oxysporum f. Sp. cubense) Causal Agent of Banana Wilt Disease in Ethiopia

Mekanisme Parasitisme Trichoderma Harzianum Terhadap Fusarium Oxysporum Pada Semai Acacia Mangium

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