Effects on Alimentary High-Fat Diet on Thiol Disulfide Homeostasis in Rats

Abstract

The effects of high-fat diet on the thiol disulfide homeostasis of rats were evaluated by activities of glutathione redox system functioning in the blood and liver. A short-term (30 days) high-fat diet inhibited activities of glutathione-dependent enzymes. High-fat loading for 90 days promoted the development of a compensatory response of the glutathione redox system and restoration of the thiol disulfide homeostasis. The compensatory processes in the glutathione system failed on day 180 of alimentary loading because of redox imbalance in the thiol disulfide metabolism.

Interaction between the Effects of Sustained Swimming Activity and Dietary Macronutrient Proportions on the Redox Status of Gilthead Sea Bream Juveniles (Sparus aurata L.)

“…The lipid storage function of the liver and the preference of red muscle to use fatty acids as fuel explain these tissue-dependent increases [ 31 , 32 ]. These results agree with those of studies showing that a higher lipid content in feed increases both lipid and protein peroxidation markers from 10 to 90% [ 4 , 6 , 11 ]. Interestingly, the reduced AOPP levels in the HP groups could be correlated with the lower nitrogen fractionation (Δδ 15 N) found in the white muscle of the same fish analyzed in this work [ 18 ].…”

“…However, the opposite results were shown by Lasker et al (2019) [11], who observed that a high-fat diet reduced the activities of SOD and CAT. This is in line with the results observed in murine models by Karaman et al (2013) [6] for reduced thiols and by Jarukamjorn et al (2016) [57] for relative expression and enzyme activity, with an initial upregulation of protective mechanisms that is exceeded and depressed after a while.…”

“…Increasing the levels of dietary energy proceeding from lipids is associated with a higher fat deposition, particularly in the liver, adipose tissue and muscle. It is well established that raising the dietary lipid content leads to the production of reactive oxygen species (ROS) through diverse pathways, generating downstream molecules such as malondialdehyde (MDA), advanced oxidation protein products (AOPP) and oxidized thiols, which can be used as biochemical markers of the tissue redox status in fish [ 6 , 7 ]. Marine fish feed is rich in polyunsaturated fatty acids (PUFAs), which accumulate in tissues and provide a healthy meal for human consumption.…”

Investigation of Thiol/Disulfide Balance in Obese Rats with Non-Alcoholic Fatty Liver Disease

“…Karaman et al [ 30 ] investigated the effects of high fat diet on the thiol-disulfide metabolism of the rats. They found that the compensatory response failed in the glutathione and the impairment of redox imbalance in thiol/disulfide metabolism after 180 days alimentary.…”

Effect of cassava flour on the lipidic and redox profile of Wistar rats with dyslipidemia

“…Sulfhydryls are characterized as SH‐ thiol groups that act as antioxidants in the body, such as glutathione and when present in proteins and other compounds can be oxidized in the presence of free radicals (Halliwell & Gutteridge, 2007). Karaman and cols demonstrated that rats submitted to a high‐fat diet presented a recover of glutathione levels at 90 days, but not at 30 or 180 days, as well as an improvement in glutathione reductase (Karaman, Novgorodtseva, & Yan, 2013). In our study, the sulfhydryl content in liver and plasma of treated animals were increased, which it is possible a compensatory response of antioxidant system stimulated by the consumption of fat and cholesterol.…”