Effects of Δ8-Tetrahydrocannabinol, Δ9-Tetrahydrocannabinol, and Crude Marihuana on Human Cells in Tissue Culture

Stenchever, Morton A.; Parks, Kathryn J.; Stenchever, Marc R.

doi:10.1007/978-3-642-51624-5_22

Cited by 7 publications

(1 citation statement)

References 8 publications

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“…Similar negative results were reported in the in vitro studies of Neu et al (12) and Stenchever and Allen (18). More recently Stenchever et al (20) reported that A 8-THC and A 9-THC did not induce abnormalities in leukocytes in vitro, although treatment with crude marihuana ex tract significantly increased aneuploidy. Chro mosomal abnormalities have been reported in lung explants exposed to marihuana smoke (6).…”

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confidence: 70%

Influence of Cannabinoids on Somatic Cells <i>in vivo</i>

Zimmerman¹,

Raj²

1980

Pharmacology

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Chromosomal and nuclear aberrations in bone marrow cells were studied in hybrid mice of genotype (C57BL x C3H)F₁ following treatment with specific cannabinoids. In the subacute series, mice were treated for 5 consecutive days with Δ⁹-tetrahydrocannabinol (THC), cannabinol or cannabidiol at a dose of 10 mg/kg, the percentage of micronuclei in cannabinoid-treated mice was 3- to 5-fold greater than in the dimethyl sulfoxide controls. In the acute series, polychromatic erythrocytes were scored after a single exposure. In one acute series there was an approximately twofold increase in the incidence of micronuclei in the cannabinoid-treated mice; in the other acute series of cannabinoid-treated animals the micronuclei values were as high as 6 times the control values. Interaction between dose and frequency of exposure was assessed for THC; the number of micronuclei was affected by THC dosage, but not by frequency of exposure. Further confirmation of nuclear aberrations was obtained from assessment of bone marrow mitosis. In this limited study of mitotic cells the incidence of metaphase aberrations in cannabinoid-treated animals was 5–7 times greater than in controls.

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supporting

confidence: 70%

Influence of Cannabinoids on Somatic Cells <i>in vivo</i>

Zimmerman¹,

Raj²

1980

Pharmacology

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In vitro induction of segregational errors of chromosomes by natural cannabinoids in normal human lymphocytes

1980

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It has been shown that segregational errors (SE) of chromosomes can be induced by olivetol and several halogenated inhalation anesthetics. The purpose of this study was to examine the effects of natural cannabinoids - including delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), and cannabidiol (CBD) - on chromosome segregation. Lymphocytes obtained from healthy adult males were incubated with various concentrations of natural cannabinoids for 72 hours. Anaphase preparations were made from these cultures. A statistically significant increase in the incidence of SE of chromosomes was observed in the lymphocytes exposed to THC at a concentration of 3.2 x 10(-6) M, but not to CBN or CBD. A greater incidence of bridge formations, anaphase lags, micronuclei, and unequal segregations in bipolar divisions and multipolar divisions were observed in THC-treated lymphocytes, compared with the controls. However, only anaphase lags and unequal segregations in bipolar divisions reached statistically significant levels. It appears that THC affects the formation of microtubules and spindles and may be considered as a mitotic poison. The value of examining SE as a part of cytogenetic studies on chemical mutagens is emphasized. A description of a classification system of SE developed in our laboratory is also presented. This system can be applied to studies using both normal human lymphocyte cultures and lymphoid cell lines.

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Drug Abuse Effects on the Reticuloendothelial and Immune Systems

Lefkowitz

1985

Pharmacology

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Effects of Δ8-Tetrahydrocannabinol, Δ9-Tetrahydrocannabinol, and Crude Marihuana on Human Cells in Tissue Culture

Cited by 7 publications

References 8 publications

Influence of Cannabinoids on Somatic Cells <i>in vivo</i>

Influence of Cannabinoids on Somatic Cells <i>in vivo</i>

In vitro induction of segregational errors of chromosomes by natural cannabinoids in normal human lymphocytes

Drug Abuse Effects on the Reticuloendothelial and Immune Systems

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