Effects of β-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis

Drift, S.G.A. van der; Everts, R.R.; Houweling, Martin; Leengoed, L.A.M.G. van; Stegeman, Jan Arend; Tielens, Aloysius G.M.; Jorritsma, R.

doi:10.1016/j.rvsc.2012.11.009

Cited by 10 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further investigate the mechanisms by which HS affects the response of adipocytes to lipolytic signals, the abundance and phosphorylation of several proteins involved in the regulation of lipolysis were evaluated. The role of HSL in the lipolytic pathway is well documented, and its activity is regulated by phosphorylation and translocation to the lipid droplet (Yin et al, 2003;Koltes and Spurlock, 2011;van der Drift et al, 2013). In particular, HSL is activated when phosphorylated at serine residue 563 by protein kinase A (PKA; Ronchi et al, 1999;Roepstorff et al, 2004) although several other phosphorylation sites may also be involved (Anthonsen et al, 1998;Holm et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Effects of acute heat stress on lipid metabolism of bovine primary adipocytes

Faylon

Baumgard

Rhoads

et al. 2015

Journal of Dairy Science

View full text Add to dashboard Cite

Heat stress (HS) affects numerous physiological processes including nutrient partitioning and lipid metabolism. Objectives of this study were to evaluate how acute HS affects lipid metabolism in subcutaneous adipose tissue of dairy cattle. Adipose tissue biopsies were performed on Holstein cows for bovine primary adipocyte isolation and cultured at either 42°C (HS) or 37°C (thermal neutral, TN). Adipocytes were incubated with increasing isoproterenol (ISO), and with increasing concentrations of insulin in the presence of ISO to evaluate changes in lipolysis. Incorporation of radioactive acetate into lipids was measured as an indicator of lipogenesis. Abundance and phosphorylation of several lipolytic and lipogenic proteins were also measured. Adipocytes exposed to HS had an elevated maximal response to ISO and were more sensitive to lipolytic stimulation by ISO compared with cells cultured at TN. Thermal treatment did not affect the antilipolytic effects of insulin in the presence of ISO. Lipogenesis measured as acetate incorporation was not altered by HS, but a temperature by insulin interaction was observed for the regulation of acetyl CoA carboxylase, such that the presence of insulin resulted in a reduction in phosphorylation of acetyl CoA carboxylase in adipocytes cultured at TN but not HS conditions. Results of this study demonstrate that acute HS has a direct effect on the regulation of lipolysis and the rate-limiting enzyme of lipogenesis in isolated bovine adipocytes.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of acute heat stress on lipid metabolism of bovine primary adipocytes

Faylon

Baumgard

Rhoads

et al. 2015

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…The higher BHB concentration in CEI cows than in HEI cows at d 42 and 56 indicates compensation of insufficient supply of glucose precursors, as suggested by lower concentrate DMI. The higher BHB concentration may have also served as a tissue-protective mechanism in CEI cows because BHB has been shown to inhibit basal AT lipolysis in vitro in a dose-dependent matter (van der Drift et al, 2013). Thus, the feedback mechanisms may have prevented toxic effects of high BHB levels on tissues by shutting down further release of additional NEFA (Rukkwamsuk et al, 1998).…”

Section: Plasma Metabolites and Hormonesmentioning

confidence: 99%

Effects of dry period energy intake on insulin resistance, metabolic adaptation, and production responses in transition dairy cows on grass silage–based diets

Salin

Vanhatalo

Jaakkola

et al. 2018

Journal of Dairy Science

View full text Add to dashboard Cite

High energy intake in the dry period has reportedly had adverse effects on mobilization of body reserves, dry matter intake, and productivity of dairy cows. We investigated whether grass silage (GS) fed ad libitum (high energy intake, HEI; 141% of daily metabolizable energy requirements) in an 8-wk dry period affects metabolic adaptation-specifically, peripheral insulin resistance-compared with a total mixed ration consisting of GS, wheat straw, and rapeseed meal (55/40/5%; controlled energy intake, CEI; 108% of metabolizable energy/d) fed ad libitum. Multiparous Ayrshire dairy cows (n = 16) were used in a randomized complete block design until 8 wk after parturition. Commercial concentrates were fed 1 and 2 kg/d during the last 10 to 6 and 5 to 0 d before the expected calving date, respectively. Postpartum, a similar lactation diet with ad libitum access to GS and increasing concentrate allowance (maximum of 16 kg/d) was offered to all. The HEI group gained more body weight and had higher plasma insulin, glucose, and β-hydroxybutyrate concentrations than the CEI group prepartum. Postpartal plasma glucose tended to be higher and milk yield was greater from wk 5 onward for HEI compared with CEI cows. An intravenous glucose tolerance test (IVGTT) was performed at −13 ± 5 d and 9 ± 1 d relative to calving. The HEI cows had greater insulin response to glucose load and smaller area under the response curve for glucose than CEI cows in prepartal IVGTT. Thus, compensatory insulin secretion adapted to changes in insulin sensitivity of the peripheral tissues, preserving glucose tolerance of HEI cows. Higher insulin levels were needed in HEI cows than in CEI cows to elicit a similar decrement of nonesterified fatty acid concentration in prepartal IVGTT, suggesting reduced inhibition of lipolysis by insulin in HEI cows before parturition. In conclusion, high energy intake of moderately digest-ible GS with low concentrate feeding in the close-up dry period did not have adverse effects on metabolic adaptation, insulin sensitivity, and body mobilization after parturition. Instead, this feeding regimen was more beneficial to early-lactation performance than GS-based total mixed ration diluted with wheat straw.

show abstract

“…Over 60% of all dairy cows develop ketosis or fatty liver during the transition period, which is a direct response to an energy imbalance and may decrease hepatic efficiency, animal health, and milk production ( 3 , 4 ). Excessive NEB initiated fat mobilization of adipose tissues and a subsequent increase in blood concentrations of non-esterified fatty acid (NEFA) and ketone bodies ( 5 ). Therefore, the lipid metabolism of adipose tissue in cows with ketosis or fatty liver exhibited a disordered state.…”

Section: Introductionmentioning

confidence: 99%

Perilipin 1 Mediates Lipid Metabolism Homeostasis and Inhibits Inflammatory Cytokine Synthesis in Bovine Adipocytes

Zhang

Liu

et al. 2018

Front. Immunol.

View full text Add to dashboard Cite

Dairy cows with ketosis displayed lipid metabolic disorder and high inflammatory levels. Adipose tissue is an active lipid metabolism and endocrine tissue and is closely related to lipid metabolism homeostasis and inflammation. Perilipin 1 (PLIN1), an adipocyte-specific lipid-coated protein, may be involved in the above physiological function. The aim of this study is to investigate the role of PLIN1 in lipid metabolism regulation and inflammatory factor synthesis in cow adipocytes. The results showed that PLIN1 overexpression upregulated the expression of fatty acid and triglyceride (TAG) synthesis molecule sterol regulator element-binding protein-1c (SREBP-1c) and its target genes, diacylglycerol acyltransferase (DGAT) 1, and DGAT2, but inhibited the expression of lipolysis enzymes hormone-sensitive lipase (HSL) and CGI-58 for adipose triglyceride lipase (ATGL), thus augmenting the fatty acids and TAG synthesis and inhibiting lipolysis. Importantly, PLIN1 overexpression inhibited the activation of the NF-κB inflammatory pathway and decreased the expression and content of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) induced by lipopolysaccharide. Conversely, PLIN1 silencing inhibited TAG synthesis, promoted lipolysis, and overinduced the activation of the NF-κB inflammatory pathway in cow adipocytes. In ketotic cows, the expression of PLIN1 was markedly decreased, whereas lipid mobilization, NF-κB pathway, and downstream inflammatory cytokines were overinduced in adipose tissue. Taken together, these results indicate that PLIN1 can maintain lipid metabolism homeostasis and inhibit the NF-κB inflammatory pathway in adipocytes. However, low levels of PLIN1 reduced the inhibitory effect on fat mobilization, NF-κB pathway, and inflammatory cytokine synthesis in ketotic cows.

show abstract

Effects of β-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis

Cited by 10 publications

References 24 publications

Effects of acute heat stress on lipid metabolism of bovine primary adipocytes

Effects of acute heat stress on lipid metabolism of bovine primary adipocytes

Effects of dry period energy intake on insulin resistance, metabolic adaptation, and production responses in transition dairy cows on grass silage–based diets

Perilipin 1 Mediates Lipid Metabolism Homeostasis and Inhibits Inflammatory Cytokine Synthesis in Bovine Adipocytes

Contact Info

Product

Resources

About