Effects of α-linolenic acid on colonic secretion in rats with experimental colitis

Shimizu, Toshiaki; Kitamura, Toshiya; Suzuki, Mitsuyoshi; Fujii, Tohru; Shoji, Hiromichi; Tanaka, Kyoko; Igarashi, Jun

doi:10.1007/s00535-006-1998-4

Cited by 5 publications

(5 citation statements)

References 27 publications

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“…In animal models, the PSO supplementation was found to decrease the level of arachidonic acid yet increase the level of ALA, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) in the colon. The combination of PSO with lipoxygenase inhibitor suppresses the increase of chloride ion secretion-related diarrhea in mice’s colitis ( 20 ). Furthermore, the advantage of PSO is that it is inexpensive, free of heavy metal contamination, and safe ( 21 ).…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effect of Perilla frutescens seed oil rich in omega-3 fatty acid on dextran sodium sulfate-induced colitis in mice

et al. 2021

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Background and purpose: Ulcerative colitis is a chronic inflammatory bowel disease that involves diffused inflammation of the large intestine. Omega-3 fatty acid (FA) has been known to regulate the inflammatory response associated with ulcerative colitis pathogenesis. Perilla frutescens is a valuable source of omega-3 FA and α-linolenic acid (ALA) contained in its seed oil. Therefore, the aim of this study was to evaluate the anti-inflammatory effect of Perilla seed oil (PSO) on colitis induced by dextran sulfate sodium (DSS) in a mouse model. Experimental approach: PSO was extracted using a cold-pressed extractor and FA composition of PSO was analyzed by GC-MS. Acute colitis in mice was induced with 3% DSS in drinking water for 7 days. Some mice were treated with PSO (20, 100, 200 mg/kg BW) for 3 weeks before the DSS administration. Sulfasalazine was used as a positive control. The clinical features, histopathologic, serum, and gene expression of proinflammatory cytokines in the colon were assessed. Finding/Results: PSO contained the highest proportion of ALA (61.51%). Furthermore, PSO pretreatment evidently reduced body weight loss, diminished diarrhea, gross bleeding, and DSS-induced colon shortening. PSO pretreatment attenuated histopathological changes in response to DSS-induced colitis. PSO pretreatment also markedly decreased inflammatory response in serum and the colon tissue of DSS-induced mice. Conclusion and implication: ALA in PSO is suggested to be mainly responsible for the reduction of DSS-induced colitis through suppressing inflammatory markers. PSO could be further developed as a functional health supplement, which would be beneficial for anti-inflammation in the colonic mucosa.

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Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effect of Perilla frutescens seed oil rich in omega-3 fatty acid on dextran sodium sulfate-induced colitis in mice

et al. 2021

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“…There is considerable evidence for the involvement of the 5-LOX pathway and COX-2 in the DSS model of colitis [ 9 , 11 , 12 , 13 , 30 , 31 ]. A recent study reported the measurement of lipid inflammatory mediators throughout the different phases of inflammation [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…In a DSS study using a LOX inhibitor (AA-861) and linolenic acid supplementation, the combination was able to suppress the excessive chloride secretion that results in diarrhea. Linolenic acid is metabolized to the anti-inflammatory eicosanoids PGE1 and TBA1 and prevents the formation of leukotrienes, while AA-861 is a selective 5-LOX and LTB4 inhibitor [ 13 ]. In another study, DSS caused a marked inflammatory response in the colon and a subsequent 5-fold increase in eicosanoid production [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…The products of 5-LOX, 5-HETE, and LTB4 are markedly increased in the dextran sodium sulfate (DSS) model [ 9 , 10 , 11 ]. When DSS-treated animals are given a 5-LOX inhibitor or an LTB4 antagonist, colonic shortening as well as histological and inflammatory scores were improved in the mouse model of colonic inflammation [ 9 , 11 , 12 , 13 ]. Therefore, we hypothesized that Frondanol may attenuate the inflammatory responses seen in IBD.…”

Section: Introductionmentioning

confidence: 99%

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Frondanol, a Nutraceutical Extract from Cucumaria frondosa, Attenuates Colonic Inflammation in a DSS-Induced Colitis Model in Mice

et al. 2018

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Frondanol is a nutraceutical lipid extract of the intestine of the edible Atlantic sea cucumber, Cucumaria frondosa, with potent anti-inflammatory effects. In the current study, we investigated Frondanol as a putative anti-inflammatory compound in an experimental model of colonic inflammation. C57BL/6J male black mice (C57BL/6J) were given 3% dextran sodium sulfate (DSS) in drinking water for 7 days to induce colitis. The colitis group received oral Frondanol (100 mg/kg body weight/per day by gavage) and were compared with a control group and the DSS group. Disease activity index (DAI) and colon histology were scored for macroscopic and microscopic changes. Colonic tissue length, myeloperoxidase (MPO) concentration, neutrophil and macrophage marker mRNA, pro-inflammatory cytokine proteins, and their respective mRNAs were measured using ELISA and real-time RT-PCR. The tissue content of leukotriene B4 (LTB4) was also measured using ELISA. Frondanol significantly decreased the DAI and reduced the inflammation-associated changes in colon length as well as macroscopic and microscopic architecture of the colon. Changes in tissue MPO concentrations, neutrophil and macrophage mRNA expression (F4/80 and MIP-2), and pro-inflammatory cytokine content (IL-1β, IL-6 and TNF-α) both at the protein and mRNA level were significantly reduced by Frondanol. The increase in content of the pro-inflammatory mediator leukotriene B4 (LTB4) induced by DSS was also significantly inhibited by Frondanol. It was thus found that Frondanol supplementation attenuates colon inflammation through its potent anti-inflammatory activity.

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“…Studies have shown that ALA has a strong effect on reducing risks of chronic diseases such as atherosclerosis, thrombosis and hyperlipidemia (Rodriguez-Leyva et al, 2010;Zhao et al, 2004;Winnik et al, 2011). Recently, dietary supplementation with ALA has been shown to improve colonic inflammation of experimental colitis rats (Shimizu et al, 2007;Wen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Effect of α-linolenic acid (ALA) on proliferation of probiotics and its adhesion to colonic epithelial cells

Liu¹,

Liu²,

Liu³

et al. 2022

Food Sci. Technol

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The effects of α-linolenic acid (ALA) on the proliferation and adhesion of probiotics would be investigated in the present study. Effects of ALA on intestinal flora were studied by animal fecal anaerobic fermentation system in vitro, which were analyzed by high-throughput sequencing. Results showed that treatment with ALA could promote the proliferation of probiotics Lactobacillus and Bifidobacterium, and inhibit the growth of harmful bacteria Enterococcus and E. coli. ALA restored the abnormal intestinal flora caused by high-fat diet, which was beneficial to the improvement of intestinal flora structure. In addition, adhesive characteristics of probiotics to epithelial colon cells NCM460 were detected by plate counting and Gram staining, which indicated that ALA promoted adhesion of probiotics to colonic cells. In conclusion, ALA could promote the proliferation and adhesion of intestinal probiotics, which provides a basis for ALA to exert the healthy activities of intestinal probiotics.

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Effects of α-linolenic acid on colonic secretion in rats with experimental colitis

Abstract: Our results suggest that supplementation with alpha-linolenic acid, in combination with a lipoxygenase inhibitor, could suppress the increase in Cl- secretion in patients with UC.

Cited by 5 publications

References 27 publications

Anti-inflammatory effect of Perilla frutescens seed oil rich in omega-3 fatty acid on dextran sodium sulfate-induced colitis in mice

Anti-inflammatory effect of Perilla frutescens seed oil rich in omega-3 fatty acid on dextran sodium sulfate-induced colitis in mice

Frondanol, a Nutraceutical Extract from Cucumaria frondosa, Attenuates Colonic Inflammation in a DSS-Induced Colitis Model in Mice

Effect of α-linolenic acid (ALA) on proliferation of probiotics and its adhesion to colonic epithelial cells

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