Effects of α-Hederin, a Saponin Extracted from Hedera helix, on Cells Cultured<i>in vitro</i>

Danloy, Sophie; Quetin-Leclercq, Joëlle; Coucke, Philippe; Pauw-Gillet, Marie-Caroline De; Elias, R.; Balansard, G.; Angenot, Luc; Bassleer, R

doi:10.1055/s-2006-959406

Cited by 54 publications

(45 citation statements)

References 5 publications

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“…In addition, it was demonstrated that the mitotic activity of these two cell lines was suppressed. Danloy et al (17) reported that α-hederin isolated from H. helix was cytotoxic against B16 mouse melanoma and 3T3 fibroblast cell lines. α-hederin isolated from H. helix leaves potentiated the antitumor activity of 5-fluorouracil in the HT-29 human colon adenocarcinoma cell line (28).…”

Section: Discussionmentioning

confidence: 99%

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Effects of Hedera helix L. extracts on rat prostate cancer cell proliferation and motility

Aktaş

Altun

2016

Oncology Letters

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Abstract. Hedera helix L., a member of Araliaceae family, has antiproliferative, cytotoxic, antimicrobial, antifungal, antiprotozoal and anti-inflammatory effects, and is used in cosmetics. The aim of the present study was to investigate the effect of treatment with extracts of leaves and unripened fruits of H. helix on rat prostate cancer cell lines with markedly different metastatic potentials: Mat-LyLu cells (strongly metastatic) and AT-2 cells (weakly metastatic). The effects of the extracts on cell kinetics and migration were determined. Tetrodotoxin was used to block the voltage-gated sodium channels (VGSCs) associated specifically with Mat-LyLu cells. Cell proliferation was detected spectrophotometrically using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. The mitotic index was determined using the Feulgen staining method. Lateral motility was quantified by wound-healing assays. The results of the present study demonstrated that cell kinetics (proliferation and mitotic activity) and motility were inhibited by ethanolic leaf extract of H. helix. The ethanolic extract of H. helix fruit suppressed Mat-LyLu cell migration, with no effect on proliferation. The opposite effects were observed in AT-2 cells; migration was not affected but proliferation was inhibited. In conclusion, the ethanolic fruit extract of H. helix may inhibit the cell migration of Mat-LyLu cells by blocking VGSCs. However, the effect of ethanolic leaf extract of H. helix treatment on the lateral motility of the cancer cells is unclear. IntroductionProstate cancer is the second most common cause of cancer-associated mortality among men worldwide (1). By the time prostate cancer is diagnosed, metastasis has occurred in the majority of men, as early prostate cancer has no symptoms. Metastatic diseases are a significant public health problem affecting cancer patients and their families (2). Metastasis occurs when malignant cells leave the primary tumor, migrate via the circulatory system, localize in distant areas and lead to the development of secondary tumors. It is a multistep process, and the steps are similar in all tumors. Mobilization of tumor cells is an important step in metastasis (3). Ion channels regulate, and stimulate numerous behavioral changes in cells that are associated with cancer and metastasis, including cell movement (elongation and lateral motility) (4,5), migration, galvanotaxis (6) and invasion (7,8). A number of in vitro (9-11) and in vivo (12) studies performed using tetrodotoxin (TTX), which specifically blocks voltage-gated sodium channels (VGSCs) (13), have suggested that the plasma membrane of prostate cancer cells may gain a more excitable phenotype due to increased VGSC expression, and thus malignancy is able to progress. Bennett et al (11) demonstrated that VGSC expression was 'necessary' and 'enough' for the invasiveness of prostate cancer cells. Prostate cancer tends to invade the bones, lungs and lymph nodes (14). Combating metastasis formation and growth are important for succ...

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Section: Discussionmentioning

confidence: 99%

“…Saponin derivatives obtained from Hedera spp. have numerous biological activities, including antiproliferative, cytotoxic (17,18), antibacterial (19), antifungal (20), anthelmintic (21), antileishmanial (22), anti-elastase and anti-hyaluronidase (23) effects. de Medeiros et al (24) demonstrated that H. helix spp.…”

Section: Introductionmentioning

confidence: 99%

Effects of Hedera helix L. extracts on rat prostate cancer cell proliferation and motility

Aktaş

Altun

2016

Oncology Letters

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“…␣-hederin was first isolated from the leaves of Hedera helix (3). This molecule possesses strong hemolytic activity (4,5) and cytotoxicity against in vivo tumors; in addition, it has in vitro effects on various cancer cell lines, which could result from apoptosis (2,6,7) and/or membrane alterations (5,8).…”

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confidence: 99%

Induction of Highly Curved Structures in Relation to Membrane Permeabilization and Budding by the Triterpenoid Saponins, α- and δ-Hederin

Lorent

Duff

Quetin-Leclercq

et al. 2013

Journal of Biological Chemistry

104

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Background:The triterpenoid monodesmosidic saponins, ␣-and ␦-hederin, induced membrane permeabilization. Results: The membranous cholesterol and the sugars branched on the aglycone, hederagenin, are critical for membrane permeabilization, budding, and the change in lipid phase. Conclusion: Permeabilization and budding are dependent on the interaction of saponin with cholesterol and the molecular shape of the saponin. Significance: Induction of curvature by saponins is responsible for permeabilization and budding.

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“…1) distributed in Hedera or Nigella species displays many biological activities such as hemolytic, antispasmodic (Trute et al, 1997), antiinflammatory (Choi et al, 2002a(Choi et al, , 2002bKim et al, 2002), antileishmanial (Delmas et al, 2000;Ridoux et al, 2001), molluscicidal and cytotoxic (Park et al, 2001), partially due to their interaction with the cell membrane (Chwalek et al, 2006). α-Hederin is increasingly investigated for its promising antitumor potential since it revealed cytotoxicity against various cancer cell lines (Quetin-Leclercq et al, 1992;Danloy et al, 1994;Rooney and Ryan, 2005a;Tian et al, 2006) and in vivo tumors (Kumara and Huat, 2001) with antimutagenic activity (Elias et al, 1990;Amara-Mokrane et al, 1996;Lee et al, 2000). It has been suggested that α-hederin exerts its cytotoxic activity by promoting apoptosis and/or membrane alterations, although the molecular and cellular mechanisms are far from being fully elucidated (Swamy and Huat, 2003;Rooney and Ryan, 2005b).…”

Section: Introductionmentioning

confidence: 99%

α‐hederin potentiates 5‐FU antitumor activity in human colon adenocarcinoma cells

Bun¹,

Élias²,

Baghdikian³

et al. 2008

Phytotherapy Research

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The aim of this study was to investigate the ability of alpha-hederin to improve the efficacy of widely prescribed 5-fluorouracil (5-FU) in a human colon adenocarcinoma model. Drug combinations of alpha-hederin and 5-FU using both fixed-concentration and combination index methods were performed in vitro in HT-29 cells. The results showed that alpha-hederin at sub-IC(50) cytotoxic concentrations enhanced 5-FU cytotoxicity about 3.3-fold (p < 0.001). Simultaneous combination of alpha-hederin and 5-FU at their IC(50) ratio showed either a synergistic effect at a moderate cytotoxic range (25% of cell growth inhibition) or an antagonistic effect at a high level of growth inhibition. The data indicate therefore that it is possible to optimize colorectal cancer cell sensitivity to 5-FU with alpha-hederin.

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Effects of α-Hederin, a Saponin Extracted from Hedera helix, on Cells Culturedin vitro

Cited by 54 publications

References 5 publications

Effects of Hedera helix L. extracts on rat prostate cancer cell proliferation and motility

Effects of Hedera helix L. extracts on rat prostate cancer cell proliferation and motility

Induction of Highly Curved Structures in Relation to Membrane Permeabilization and Budding by the Triterpenoid Saponins, α- and δ-Hederin

α‐hederin potentiates 5‐FU antitumor activity in human colon adenocarcinoma cells

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