Effects of zinc-bearing palygorskite on rumen fermentation in vitro

Chen, Mengjiao; Xi, Yumeng; Zhang, Lin; Zeng, Hanfang; Li, Yeqing; Han, Zhaoyu

doi:10.5713/ajas.17.0920

Cited by 11 publications

(8 citation statements)

References 30 publications

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“…According to the observations of this study, the addition of nano-ZnO on the protein source had no effect on gas production after 24 h. However, reduction and the tendency to decrease in the volume of gas production after 24 h of incubation in other study [37] are also shown. This tends to decrease with increasing the nano level, unlike results researchers [27,28] , which was up high enough so that the inhibitory effect on the activity of rumen microorganisms was shown. Between sources of animal protein, POM and BM at any of the incubation times was not affected by the addition of ZnO nanoparticles, but adding ZnO nanoparticles to FM except the first 2 h of incubation at other times had significantly effect on increasing gas production.…”

Section: Discussioncontrasting

confidence: 58%

“…Also, in an in vitro test using ZnO nanoparticles in the diet improve rumen bacterial growth and increasing the efficiency of energy intake in the diet [27] . In other experiment [28] amount of in vitro gas produced over 144 h of incubation there was no significant difference in treatments containing zinc at levels 20 and 40 ppm with control.…”

Section: Introductionmentioning

confidence: 99%

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ZnO Nanopartiküllerinin Bazı Hayvan ve Bitki Protein Kaynaklarının İn Vitro Gaz Üretimi Üzerine Etkisi

Chanzanagh¹,

Seifdavati²,

Gheshlagh³

et al. 2017

Kafkas Univ Vet Fak Derg

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This study was conducted to determine effect of adding ZnO nanoparticles at levels of 0, 30 and 60 ppm on in vitro gas production of some animal and plant protein sources. In this study, gas production at 2, 6, 12, 24, 48 and 72 h incubation were measured and 200 mg of samples were used for gas production analysis. The results showed that after 72 h of incubation, the most volume of gas production in the plant protein sources of soybean meal (SM) and in between the sources of animal protein in poultry offal meal (POM) were respectively 58.23 and 28.34 mL per 200 mg of dry matter was obtained. In related with the parameters of nutrition from incubation data, metabolizable energy (ME), for soybean meal at the levels of zero, 30 and 60 ppm ZnO nanoparticles added to the 8.55, 8.81 and 7.54 were highest and for blood meal (BM) were lowest 2.26, 2.31 and 2.01 MJ/kg dry matter (DM), respectively. The highest and the lowest amount of organic matter digestibility (DOM), short-chain fatty acids (SCFA) and microbial protein (MP) were also for SM and BM. Overall, the results showed that using levels of 0, 30 and 60 ppm of ZnO nanoparticles was no effect on in vitro gas production of some animal and plant protein sources but had no significant effect in some hours of incubation, gas production and nutrition parameters. Keywords: In vitro gas production, Nutrition parameters, Protein sources, ZnO nanoparticles ZnO Nanopartiküllerinin Bazı Hayvan ve Bitki Protein Kaynaklarınınİn Vitro Gaz Üretimi Üzerine Etkisi

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Section: Discussioncontrasting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

ZnO Nanopartiküllerinin Bazı Hayvan ve Bitki Protein Kaynaklarının İn Vitro Gaz Üretimi Üzerine Etkisi

Chanzanagh¹,

Seifdavati²,

Gheshlagh³

et al. 2017

Kafkas Univ Vet Fak Derg

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“…Zn is also involved in a wide assortment of physiological processes, so nutrient digestibility may be affected by supplemental Zn, which is incorporated into enzymes throughout the body and is critical for most metabolic processes in ruminants ( 47 ). Zinc in the diet of ruminants can substantially influence ruminal fermentation ( 48 , 49 ). Low doses of zinc (20–70 mg Zn/kg diet) only weakly affect ruminal fermentation ( 4 , 10 ), but higher doses (250–1,142 mg Zn/kg diet) can affect ruminal protozoal populations and protein degradation ( 50 ).…”

Section: Discussionmentioning

confidence: 99%

Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs

et al. 2021

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We investigated the effect of diets containing organic zinc and a mixture of medicinal herbs on ruminal microbial fermentation and histopathology in lambs. Twenty-eight lambs were divided into four groups: unsupplemented animals (Control), animals supplemented with organic zinc (Zn, 70 mg Zn/kg diet), animals supplemented with a mixture of dry medicinal herbs (Herbs, 100 g dry matter (DM)/d) and animals supplemented with both zinc and herbs (Zn+Herbs). Each lamb was fed a basal diet composed of meadow hay (700 g DM/d) and barley (300 g DM/d). The herbs Fumaria officinalis L. (FO), Malva sylvestris L. (MS), Artemisia absinthium L. (AA) and Matricaria chamomilla L. (MC) were mixed in equal proportions. The lambs were slaughtered after 70 d. The ruminal contents were used to determine the parameters of fermentation in vitro and in vivo and to quantify the microbes by molecular and microscopic methods. Samples of fresh ruminal tissue were used for histopathological evaluation. Quantitative analyses of the bioactive compounds in FO, MS, AA, and MC identified 3.961, 0.654, 6.482, and 12.084 g/kg DM phenolic acids and 12.211, 6.479, 0.349, and 2.442 g/kg DM flavonoids, respectively. The alkaloid content in FO was 6.015 g/kg DM. The diets affected the levels of total gas, methane and n-butyrate in vitro (P < 0.046, < 0.001, and < 0.001, respectively). Relative quantification by real-time PCR indicated a lower total ruminal bacterial population in the lambs in the Zn and Zn+Herbs groups than the Control group (P < 0.05). The relative abundances of Ruminococcus albus, R. flavefaciens, Streptococcus bovis, and Butyrivibrio proteoclasticus shifted in the Zn group. Morphological observation found a focally mixed infiltration of inflammatory cells in the lamina propria of the rumen in the Zn+Herbs group. The effect of the organic zinc and the herbal mixture on the parameters of ruminal fermentation in vitro was not confirmed in vivo, perhaps because the ruminal microbiota of the lambs adapted to the zinc-supplemented diets. Long-term supplementation of a diet combining zinc and medicinal herbs, however, may negatively affect the health of the ruminal epithelium of lambs.

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“…Zn is also involved in a wide assortment of physiological processes; therefore, nutrient digestibility may be affected by supplemental Zn, which is incorporated into enzymes throughout the body and is critical for most metabolic processes in ruminants [46]. It is known that zinc in the nutrition of ruminants can signi cantly in uence ruminal fermentation [47,48]. While low doses of zinc (20-70 mg Zn/kg diet) have a weak effect on rumen fermentation [4,10], higher doses (250-1142 mg Zn/kg diet) can affect the rumen protozoal population and protein degradation [49].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Zinc and/or Herbal Nutraceuticals on Rumen Fermentation, Microbiota and Histopathology in Lambs

Petrič¹,

Mravčáková²,

Kucková³

et al. 2020

Preprint

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Background: The aim of this study was to investigate the effect of diets containing organic zinc and a mixture of medicinal herbs on rumen microbial fermentation and histopathology in lambs.Methods: Twenty-eight lambs were divided into four groups: unsupplemented animals (Control), animals supplemented with organic zinc (Zn, 70 mg Zn/kg diet), animals supplemented with a mix of dry medicinal herbs (Herbs, 100 g DM/d) and animals supplemented with both zinc and herbs (Zn+Herbs). Each lamb was fed a basal diet composed of meadow hay (700 g DM/d) and barley (300 g DM/d). Fumaria officinalis L. (FO), Malva sylvestris L. (MS), Artemisia absinthium L. (AA) and Matricaria chamomilla L. (MC) were mixed in equal proportions. The lambs were slaughtered after 70 days of the experiment. The rumen content was used to determine the fermentation parameters in vitro and in vivo and to quantify the microbes by molecular and microscopic methods. Samples of fresh rumen tissue were used for histopathological evaluation.Results: Quantitative analyses of the bioactive compounds identified 3.961, 0.654, 6.482 and 12.084 g/kg DM phenolic acids and 12.211, 6.479, 0.349 and 2.442 g/kg DM flavonoids in FO, MS, AA and MC, respectively. The alkaloids content in FO was 6.015 g/kg DM. The effect of diets on values of total gas, methane and n-butyrate in vitro was observed (P < 0.046 and P < 0.001, respectively). Relative quantification by real-time PCR revealed a decrease in the total rumen bacterial population in the lambs in the Zn and Zn+Herbs groups (P < 0.05). A shift in the relative abundance of Ruminococcus albus, Streptococcus bovis, Butyrivibrio proteoclasticus and R. flavefaciens was observed in the Zn group. Morphological observation showed focal mixed infiltration of inflammatory cells within the lamina propria of the rumen in the Zn+Herbs group. Conclusion: The effect of the organic zinc and the herbal mix on rumen fermentation parameters in vitro was not confirmed in vivo because the rumen microbiota of lambs probably adapted to the zinc-supplemented diets. However, long-term supplementation of a diet combining zinc and medicinal herbs can diminish the health of the ruminal epithelium of lambs.

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Effects of zinc-bearing palygorskite on rumen fermentation in vitro

Abstract: These results suggest that the addition of Zn-Pal can improve the rumen fermentation, especially when adding 0.2% Zn-Pal.

Cited by 11 publications

References 30 publications

ZnO Nanopartiküllerinin Bazı Hayvan ve Bitki Protein Kaynaklarının İn Vitro Gaz Üretimi Üzerine Etkisi

ZnO Nanopartiküllerinin Bazı Hayvan ve Bitki Protein Kaynaklarının İn Vitro Gaz Üretimi Üzerine Etkisi

Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs

The Effect of Zinc and/or Herbal Nutraceuticals on Rumen Fermentation, Microbiota and Histopathology in Lambs

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