EFFECTS OF YUNKE (TECHNETIUM‐99 CONJUGATED WITH METHYLENE DIPHOSPHONATE; ⁹⁹Tc‐MDP) AND/OR COLLOIDAL CHROMIC PHOSPHATE PHOSPHONIUM‐32, ALONE AND IN COMBINATION, IN RATS WITH ADJUVANT ARTHRITIS

Abstract

1. The present study investigated the therapeutic effects of both single and combination treatment with Yunke (technetium-99 conjugated with methylene diphosphonate; (99)Tc-MDP) and colloidal chromic phosphate (32)P (phosphonium-32) in rats with adjuvant arthritis (AA). 2. Rats were randomly allocated to one of five groups: (i) normal control group (sham operated and treated with normal saline); (ii) AA control group (arthritis induced with adjuvant and treated with normal saline); (iii) (32)P colloid group (arthritis induced with adjuvant and treated with a single intra-articular injection of colloidal chromic phosphate phosphonium-32 (0.02 mCi) and i.p. injections of normal saline every other day); (iv) Yunke group (arthritis induced with adjuvant and treated with i.p. Yunke (2.5 x 10(-3) microg/kg) every other day and single intra-articular injection of normal saline); and (v) combination group (arthritis induced with adjuvant and treated with a combination of both therapies). 3. The left-to-right diameter (LRD) of the left hind ankle, serum levels of tumour necrosis factor (TNF) and interleukin (IL)-1b and histological sections of the ankle joints were examined at different time points. 4. The LRD of the left hind ankle was smaller for the combination group compared with (32)P colloid alone at Week 4 (7.11 +/- 0.28 vs 7.57 +/- 0.24 mm, respectively; P < 0.001). The combination treatment was more effective than (32)P colloid alone in decreasing serum TNF (1.614 +/- 0.368 vs 1.977 +/- 0.255 ng/mL, respectively; P = 0.002 for Week 4) and IL-1b (0.271 +/- 0.027 vs 0.308 +/- 0.020 ng/mL, respectively for Week 4; 0.209 +/- 0.023 vs 0.255 +/- 0.016 ng/mL, respectively for Week 6; both P = 0.001). Histologically, the combination group exhibited less synovium proliferation compared with Yunke treatment alone and decreased inflammatory cell infiltration compared with (32)P colloid alone. 5. In conclusion, the combination of Yunke and (32)P colloid is more effective in the treatment of AA in rats compared with Yunke or (32)P colloid alone.

99Tc‐methylene diphosphonate improves rheumatoid arthritis disease activity by increasing the frequency of peripheral γδ T cells and CD4+CD25+Foxp3+Tregs

“…99 Tc‐methylene diphosphonate ( 99 Tc‐MDP), a chemical compound consisting of technetium‐99 conjugated with methylene diphosphonate, has been widely used in China for the treatment of RA. Studies in both collagen‐induced arthritis (CIA) rats and RA patients have shown that 99 Tc‐MDP is effective in suppressing inflammation and bone erosion as well as reducing the production of serum TNF‐α and IL‐1β …”

Technetium-99 Conjugated with Methylene Diphosphonate Inhibits RANKL-induced Osteoclastogenesis

“…[18][19][20] It was also demonstrated in animal models that 99 Tc-MDP can reduce bone erosion 21 decrease the activity of several MMPs, control the decomposition of soft tissue and rehabilitate destroyed soft tissues. 22 However, the mechanism underlying the therapeutic effects of 99 Tc-MDP has not been completely delineated. In the present study we investigated the effects of 99 Tc-MDP on RANKL-induced osteoclastogenesis so as to explore the underlying mechanisms.…”

Nuclear and radiochemistry in China: present status and future perspectives

“…The complex is accumulated in both kidney and liver and is fast excreted. Wang et al investigated the therapeutic effects of both single and combination treatment with 99 Tc-MDP ( 99m Tc-conjugated with methylene diphosphonate, commercially named Yunke) and colloidal chromic phosphate 32 P (phosphonium-32) in rats with adjuvant arthritis (AA) [40]. It was found that a combination of 99 Tc-MDP and 32 P colloid is more effective in the treatment of AA in rats compared with 99 Tc-MDP or 32 P colloid alone.…”

“…The targets of the survey included water body, sludge, fish and soil along the river. For each sample, the quantities of total α, total β, U, Th, 226 Ra, 210 Po, 40 K, 3 H, 90 Sr, 137 Cs and 239 Pu were tested [22]. By the 1990s, Chinese researchers began to study the diffusion and migration of radioactive elements in the environmental media.…”