Effects of yogurt containing Lactobacillus plantarum HOKKAIDO on immune function and stress markers

Nishimura, Mie; Ohkawara, Tatsuya; Tetsuka, Kyohei; Kawasaki, Yo; Nakagawa, Ryoji; Satoh, Hiroki; Sato, Yuji; Nishihira, Jun

doi:10.1016/j.jtcme.2015.07.003

Cited by 34 publications

(18 citation statements)

References 22 publications

(25 reference statements)

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“…Although the mechanism of L. reuteri induction by feeding of L. plantarum AN1 cells is not clear, AN1 cells, L. reuteri , and the host immune reactions in the digestive tract might interact directly and/or indirectly. Immune improvement effects (Nishimura et al, ; Ren et al, ; Xie et al, ) and protection against infection (Hirano et al, ; Ishikawa et al, ; Liu et al, ) have also been reported for L. plantarum strains. Further study is needed to elucidate the interactions among AN1 cells, L. reuteri , and the host immune system.…”

Section: Resultsmentioning

confidence: 97%

Induction of gut Lactobacillus reuteri in normal ICR mice by oral administration of L. plantarum AN1

et al. 2018

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To clarify the existence of indigenous intestinal gut bacteria sensitive to orally administered probiotic strains of lactic acid bacteria, the effects of live and heat‐killed Lactobacillus plantarum AN1 cells, administered to normal ICR mice via drinking water were observed. Amplicon sequencing of the 16S rDNA (V4) of caecal contents revealed that the live AN1 cells increased the abundance of an indigenous sensitive gut bacterium (SGB) Lactobacillus reuteri. From faecal samples, five typical dominant colonies were detected on BL agar. Among the typical colonies, smooth brown colonies were detected only in mice fed live AN1 cells, and small brown colonies with a black centre were detected in mice fed live and heat‐killed AN1 cells. These two colony types were determined to be L. plantarum and L. reuteri, respectively. Three other typical dominant colonies were detected in all mouse groups, and were determined to be Lactobacillus intestinalis, Lactobacillus gasseri, and L. reuteri, respectively. These results suggest that non‐dominant L. reuteri is an L. plantarum‐SGB in ICR mice. Practical applications The non‐dominant L. reuteri is an L. plantarum‐SGB in ICR mice, and it is possible to demonstrate functionality with orally administered probiotics. Isolation and application of human or livestock SGB is considered promising for the development of new functional foods.

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Section: Resultsmentioning

confidence: 97%

Induction of gut Lactobacillus reuteri in normal ICR mice by oral administration of L. plantarum AN1

et al. 2018

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“…The sample size was statistically determined to obtain a power of 80% and a two-sided significance level of 5%. Based on the data of the previous clinical trial of the HOKKAIDO strain [19], to demonstrate a difference in the NK cell activity at week 8, which was postulated to have an intergroup difference of 7.5% with a standard deviation of 10%, a sample size of 60 (30 in each group) was required. Assuming a 15% loss in the follow-up rate, 70 subjects (35 in each group)…”

Section: Sample Sizementioning

confidence: 99%

“…A study showed that the HOKKAIDO strain increased the IL-12 mRNA expression level in human dendritic cells [18]. In addition, a previous clinical study in subjects aged <50 years showed that the daily consumption of yogurt containing the HOKKAIDO strain for 56 days significantly increased the NK cell activity compared with the NK cell activity of a placebo food group [19].…”

Section: Introductionmentioning

confidence: 99%

Effect of Daily Intake of Heat-Killed Lactobacillus plantarum HOKKAIDO on Immunocompetence: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Study

Haseda¹,

Nishimura²,

Sugawara³

et al. 2020

Bioactive Compounds in Health and Disease

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Background: Consumption of Lactobacillus plantarum HOKKAIDO (HOKKAIDO strain) reportedly increases immunocompetence. However, the concentration of viable bacteria and the type of food they can be incorporated into limits their consumption. The study aims to demonstrate the effect of daily intake of the heat-killed L. plantarum HOKKAIDO strain (HK-HOKKAIDO strain; 5 × 1010 colony-forming units/day) by healthy subjects with low natural killer (NK) cell activity for 8 weeks.Method: In this randomized, double-blind, placebo-controlled, parallel-group study, 70 healthy Japanese subjects who showed relatively low NK cell activity were recruited and randomly assigned to the HK-HOKKAIDO strain or placebo group. All subjects ingested one capsule per day for 8 weeks. We conducted medical interviews and performed body composition measurements, vital sign examinations, and blood sampling at weeks 0 (baseline), 4, and 8, and we collected salivary samples at weeks 0 and 8. In addition, the frequency and severity of cold symptoms in the subjects were recorded daily during the intake period.Results: Intake of the HK-HOKKAIDO strain did not increase the NK cell activity or immunity marker levels, including those of immunoglobulin, leukocyte fraction, and salivary secretory immunoglobulin A. However, the frequency and severity of the common cold symptoms were significantly reduced after the daily consumption of the HK-HOKKAIDO strain.Conclusions: The results showed that HK-HOKKAIDO strain administration can decrease the frequency and severity of common cold symptoms in healthy subjects. Our findings support the use of the HK-HOKKAIDO strain as a functional food with health benefits.Clinical trial registration: UMIN000034822

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“…This makes dairy products a rich source for LAB screening. Traditional yogurts constitute a major source for the isolation of these probiotic microorganisms (Simova et al 2009;Abd El Gawad et al 2010;Nishimura et al 2016;Sharifi Yazdi et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Mining of lactic acid bacteria from traditional yogurt (Mast) of Iran for possible industrial probiotic use

Marhamatizadeh

Sayyadi

2019

Italian Journal of Animal Science

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Lactobacillus is a genus of gram-positive bacteria of high probiotic value. The bacteria of this genus are responsible for maintaining and promoting the microbial balance of the human gut, where they along with promoting better digestion and absorption, also help in suppressing many infectious diseases. Iranian traditional yogurt (Mast) is a rich source of Lactobacillus microorganisms and their identification can be explored for their use in industrial probiotics. This study is aimed at isolation and identification of different Lactobacillus strains present in the Mast. Forty samples of Mast from eight different regions of the Iran were cultured in De Man, Rogosa and Sharpe agar (MRS) media for isolation. The isolated strains were identified by morphological and biochemical methods using gram staining, catalase, oxidase, motility and indole tests. For biochemical identification, the isolates were subjected to carbohydrate fermentation test using phenol red broth base. Overall eight strains were majorly identified based on biochemical and morphological screening which were further confirmed using 16S rDNA-based identification. Two strain of Lactobacillus acidophilus, two strains of Lactobacillus bulgaricus, two strains of Lactobacillus delbrueckii and two strains of Lactobacillus casei were identified. Lactobacillus delbrueckii subsp. Bulgaricus isolated from Bushkan showed higher bile salt resistant activities (18.92, 13.56 and 10.22%) than that of the available Lactobacillus bulgaricus strain (3.08, 1.87 and 1.44%) in 0.3, 0.5 and 1.0% bile salt. This study thus provides insights into probiotic potential of Mast which could be explored in industrial yogurt and cheese production. HIGHLIGHTS Insight into Lactic Acid Bacteria profile of traditional yogurt of Iran. The Lactic Acid Bacteria profile varies from region to region. Proposes possible probiotics for commercial yogurt production from traditional yogurt of Iran.

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Effects of yogurt containing Lactobacillus plantarum HOKKAIDO on immune function and stress markers

Cited by 34 publications

References 22 publications

Induction of gut Lactobacillus reuteri in normal ICR mice by oral administration of L. plantarum AN1

Induction of gut Lactobacillus reuteri in normal ICR mice by oral administration of L. plantarum AN1

Effect of Daily Intake of Heat-Killed Lactobacillus plantarum HOKKAIDO on Immunocompetence: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Study

Mining of lactic acid bacteria from traditional yogurt (Mast) of Iran for possible industrial probiotic use

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