Effects of yam tuber protein, dioscorin, on attenuating oxidative status and learning dysfunction in d-galactose-induced BALB/c mice

Han, Chuan Hsiao; Lin, Yuh Feng; Lin, Yin Shiou; Lee, Tai Lin; Huang, Wei; Lin, Shyr Yi; Hou, Wen Chi

doi:10.1016/j.fct.2014.01.012

Cited by 32 publications

(28 citation statements)

References 30 publications

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“…The ROS-scavenging abilities and the anti-inflammatory activities of citrus flavonoids show neuroprotective effects in vitro (Hwang et al 2012). Therefore, there are several reports concerning radical scavenging and antioxidant activities from natural resources, such as yam dioscorin and its synthesized peptides from in silico pepsin hydrolysis (Han et al 2013, 2014a, b, c), the hydrolysable tannin of geraniin (Lin et al 2008), and curcuminoids (Chen et al 2006; Feng and Liu 2009; Zhao et al 2011; Liu et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in d-galactose-induced BALB/c mice in vivo

et al. 2019

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Background There were few report concerning anti-glycation and antioxidant activities of the minor amounts of components in curcuminoids, demethylcurcumin and tetrahydroxycurcumin, in vitro and in vivo. Results The bovine serum albumin/galactose of non-enzymatic glycation models, radical-induced hemolysis, and oxygen radical absorbance capacity (ORAC) were studied in vitro, and the d-galactose-induced oxidative stress in BALB/c mice and then demethylcurcumin or tetrahydroxycurcumin interventions in vivo. The parameters of oxidative stress in plasma and brain extracts were determined among animal groups with or without both curcuminoids interventions. The demethylcurcumin and tetrahydroxycurcumin exhibited anti-glycation, anti-hemolysis, and ORAC activities, and showed much better and significant difference (P < 0.05) compared to those of curcumin in vitro. In animal experiments, the intervened two curcuminoids at both concentrations showed to lower serum malondialdehyde (MDA), brain MDA levels and iNOS protein expressions, and elevate serum ORAC activities, and showed difference (P < 0.05) compared to the galactose-induced control. Conclusion The demethylcurcumin and tetrahydroxycurcumin showed potentials in developing functional foods for antioxidant-related purposes.

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Section: Introductionmentioning

confidence: 99%

Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in d-galactose-induced BALB/c mice in vivo

et al. 2019

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“…The dioscorin and its peptic hydrolysates at doses of 40 mg/kg were showed to have antihypertensive activities against spontaneously hypertensive rats (SHR) (Lin et al [2006]); the dioscorin at doses of 20 mg/kg or 80 mg/kg showed to lower oxidative stress of BALB/c mice induced by subcutaneous galactose injections (Han et al [2014b]). The obesity may play the central role in MS which the renin-angiotensin system in dysfunctional adipocytes will initiate inflammation and dyslipidemia, increase blood pressure and decrease insulin sensitivity accompanied with abnormal blood glucose (Frigolet et al [2013]).…”

Section: Discussionmentioning

confidence: 99%

“…The tubers of yam storage protein, dioscorin, account for about 90% of the extractable water-soluble proteins from different species as estimated by the immune staining method (Hou et al [2000]). The yam dioscorin and/or its peptic hydrolysates have been reviewed for biological activities in vitro and/or in vivo (Lu et al [2012]), among which the antihypertensive activities (Hsu et al [2002]; Lin et al [2006]; Liu et al [2009a],[b]; Lin et al [2014]) and antioxidant activities (Hou et al [2001]; Liu et al [2006]; Han et al [2013], [2014a],[b],[c]) may involve in MS studies. Therefore, a HF diet which the fat composition provide 60% of total calories is used to induce obese rats and then yam dioscorin is intervened to observe the changes of body weights, blood pressures, and glucose tolerances.…”

Section: Introductionmentioning

confidence: 99%

Effects of yam dioscorin interventions on improvements of the metabolic syndrome in high-fat diet-induced obese rats

Shih

Lin

et al. 2015

Bot Stud

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BackgroundThe metabolic syndrome (MS) is termed a cluster of multiple metabolic risk criteria which is positively correlated with cardiovascular disease and type 2 diabetes mellitus (DM). Yam dioscorins have been reported to exhibit biological activities, however, little is known their preventive effects on the MS. Therefore, a high-fat (HF) diet was used to induce Wistar rat obesity and then yam dioscorin (50 mg/kg, dio50) was intervened daily concurrent HF diet (HF diet + dio50) for five weeks to check the changes of weights of body and tissues, blood pressures, and impaired glucose tolerances. The in vitro peptic hydrolysates of dioscorin with molecular mass between 3 kDa and 10 kDa and less than 3 kDa were used to determine dipeptidyl peptidase IV (DPP IV) inhibitory activities which DPP IV inhibitor has been reported to prevent and treat type 2 DM.ResultsThere were no significant difference in body weights, feed intakes, feed conversion, and weights of adipose tissues of obese rats in groups of HF and (HF diet + dio50). However, the systolic blood pressures in obese rats of 2-, 3- and 4-week dioscorin interventions were showed significantly lower (P < 0.05) compared to the HF group. The dioscorin intervention (HF+ dio50) was showed significantly different (P < 0.05) and improved the impaired glucose tolerances compared to HF group in obese rats by the oral glucose tolerance tests. It was also found that the fraction with different molecular mass of dioscorin peptic hydrolysates (5 mg/ml) showed inhibitory activities against DPP IV using sitagliptin phosphate as positive controls.ConclusionsYam dioscorins exhibit improved MS activities in obese rats which the related mechanisms may need further investigations.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-015-0084-8) contains supplementary material, which is available to authorized users.

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“…Therefore, the development of antioxidants has become an important direction of the pharmaceutical field. Plant proteins demonstrate multiple biological activities, such as antioxidant [7], antitumor [8], immunomodulatory [9] and hypoglycemic activity [10]. There are some antioxidant proteins that have been extracted from plant material, such as seeds from Chinese chives [11], camellia [12], and Toona sinensis [13].…”

Section: Introductionmentioning

confidence: 99%

Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi

Zuo

Liu

et al. 2018

Molecules

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Semen Allii Fistulosi (PSAF) is the seed of Allium fistulosum L. of the Liliaceae family. The purpose of this study was to extract, characterize, and evaluate the antioxidant activity in vitro of proteins. Using single factor and orthogonal design, the optimum conditions of extraction were determined to be as follows: extraction time 150 min, pH 8.5, temperature 60 °C, and ratio (v/w, mL/g) of extraction solvent to raw material 35. The isoelectric point of the pH was determined to be about 4.4 and 10.2, by measuring the protein content of PSAF solutions at different pH values. The amino acid composition of PSAF was determined by high performance liquid chromatography (HPLC), and the results suggested that the species of amino acids contained in the PSAF was complete. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE) analysis showed the molecular weight was mainly between 40 and 55 kDa, and Fourier-transform infrared spectroscopy (FTIR) characterized prevalent protein absorption peaks. PSAF exhibited potent scavenging activities against DPPH assays, via targeting of hydroxyl and superoxide radicals, while chelating Fe2+ activity and demonstrating weak reducing power. This work revealed that PSAF possessed potential antioxidant activity in vitro, suggesting potential for use of PSAF as a natural antioxidant.

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Effects of yam tuber protein, dioscorin, on attenuating oxidative status and learning dysfunction in d-galactose-induced BALB/c mice

Cited by 32 publications

References 30 publications

Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in d-galactose-induced BALB/c mice in vivo

Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in d-galactose-induced BALB/c mice in vivo

Effects of yam dioscorin interventions on improvements of the metabolic syndrome in high-fat diet-induced obese rats

Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi

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